FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156871
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC23303820.143818973766304No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT858727.422780932359788No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC858457.420447050708333No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG622045.3769175647068685No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT323932.800052901317433No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG164031.4178763232892864No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT108490.937788223578947No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC108200.9352814618051623No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC102310.8843682657789849No Hit
CTTACCCGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC99670.8615480896314281No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC94890.8202297403945643No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA87100.7528929327470392No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG69460.6004126648520016No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC55650.4810389403831542No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT51490.4450798749385195No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA46600.40281068502884076No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT45610.39425311897350696No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC43570.3766193464958496No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT43490.3759278260065297No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT38430.3321891550570461No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG37800.3267434312036519No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG37570.32475530979685724No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC36510.31559266331336855No Hit
CTTACCCGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGGGGTCGAACC34240.2959707694289165No Hit
CTTACCCGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGTAGTCAAAGT34170.29536568900076154No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC34090.2946741685114416No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG33630.29069792569785224No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA29950.2588879831891369No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG29510.25508462049787745No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG25800.2230153578056672No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT25710.2222373972551823No Hit
CTTACCCGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG25570.22102723639887248No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT23900.2065917461843196No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT23480.20296126361539013No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC22540.19483589786588135No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC19210.16605135749794056No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG17920.1549005896076572No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC16090.13908205841446453No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA15800.1365752966406799No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG15760.13622953639601995No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC15560.1345007351727202No Hit
CTTACCCGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT15090.1304380522979658No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT14470.1250787685057366No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC14430.12473300826107664No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC14380.1243008079552517No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC14330.12386860764942677No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT14230.1230042070377769No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA13730.11868220397952753No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT13400.11582968196108295No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC13150.11366868043195827No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCC12820.11081615841351368No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAC12690.10969243761836886No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTC12690.10969243761836886No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG12680.10960599755720386No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCG12670.10951955749603888No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC12190.10537043456011949No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC12140.10493823425429456No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT11910.10295011284749986No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)