FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296276
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC26641820.55256750877128No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC986497.610184868037361No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT979547.556569742863403No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG714825.51441205422302No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT372282.8719192517642846No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG190821.472063048301442No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT134441.0371248098398798No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC117710.9080627890973836No Hit
CTTACCCGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC113210.8733479598480571No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC113020.8718822226130855No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC102840.793349564444609No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA102790.7929638441196165No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG77890.600875122273343No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC58030.4476670091863153No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT54240.41842940855188243No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT49520.38201740987258886No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT49020.3781602066226637No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA43690.33704241997846135No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC43590.3362709793284764No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG41900.32323363234372926No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG41430.31960786128879964No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC41240.318142124053828No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT40240.3104277175539777No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC39800.30703337869404357No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG39530.304950488939084No Hit
CTTACCCGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGGGGTCGAACC39520.30487334487408546No Hit
CTTACCCGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGTAGTCAAAGT38980.30070756536416626No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG38410.2963103536592516No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA35660.2750957357846631No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT30620.23621512702541744No Hit
CTTACCCGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG30030.23166362719050573No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG29040.2240263647556539No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT27360.2110661618359053No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT25080.19347731501624654No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC23650.18244571372146057No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC23020.17758563762655483No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG19060.1470365878871475No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC18200.14040219829727618No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA17390.13415352903239744No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC17230.13291922399242137No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA17060.1316077748874468No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC16980.1309906223674588No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG16580.12790485976751864No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAC16180.12481909716757851No Hit
CTTACCCGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT15620.12049902952766232No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT15030.11594752969275061No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT14720.11355606367779701No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC14590.11255319083281648No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC14290.11023886888286137No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC14280.11016172481786285No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC14100.10877313164788979No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC13780.1063045215679377No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAT13760.1061502334379407No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG13720.10584165717794666No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTC13650.10530164872295715No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAG13190.10175302173302599No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG13180.10167587766802749No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)