FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001132489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528192
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG110147643.56773536187125No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT85590133.854272143887805No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTG219460.8680511606713414No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGATGT213720.8453471888211023No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCAAATG131580.5204509784067033No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAATG112020.44308343670101No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGGTCGAAGT100560.3977546009163861No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG88650.3506458370250361No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGC80430.31813248360883983No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACATG79620.3149286130167329No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC73010.2887834468268233No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCACGGCCCCAGAGATCGAAGT71820.284076525833481No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGCAGT71780.28391831000177203No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCAATTG70860.2802793458724654No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC67170.2656839353973116No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC62320.24650026580259726No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTG54080.21390780447054655No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAATG52710.20848891223451382No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCCGGCCCCAGAGATCGAAGT44610.17645020631344455No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACTGCCCCAGAGATCGAAGT42520.16818342910665013No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGTAGTCAAATG40120.15869047920411108No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCATGGCCCCAGAGATCGAAGT39870.15770163025592993No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAAG38360.1517289826089158No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGGGATCGAAGT32740.1294996582538035No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGTAGTCAAATG31970.12645400349340558No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT30440.12040224793053692No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAATG30310.11988804647748272No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGATCGAAGT30170.11933429106650129No Hit
CTTACCTGAGGAGACGGTGCCCAGGGTTCCCTGGCCCCAGTAGTCAAATG26740.10576728349745589No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGACGT26700.1056090676657469No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCCATTG26460.10465977267549299No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCTG26080.10315672227425765No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG25700.10165367187302231No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAATG25380.10038794521935043No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAATG25350.1002692833455687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)