FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001132490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105052
Sequences flagged as poor quality0
Sequence length300
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGC162770777.32383808095952No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC376611.789076944417525No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACTGC230351.0942722555072273No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC161030.7649692264134093No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCACCGC142140.675232725842402No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCGC137200.6517653720668183No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGC118640.5635965287318319No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCACCGC108450.5151891734740994No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCTC84280.40037015712675983No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACAGC80950.38455107047236836No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCCCCGC72780.3457396776896723No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTTGCCCCAGTAGTCACCGC62210.29552714137227964No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCAC61790.29353194125370774No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG58700.278852968952786No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCCC55300.26270134894529923No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCACCGC46640.2215622226909359No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGCCGC43050.204508012153619No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGTAGTCACCGC42680.20275033585868663No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCACCGC42610.20241780250559133No Hit
CTTACCTGAGGAGACGGTGCCCAGGGTTCCCTGGCCCCAGTAGTCACCGC42100.1999950595044683No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCACTGC34570.16422397166435793No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTAGTCACCGC33950.161278676251228No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG32980.15667071407262148No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCCCTGC30660.14564960865574816No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGC29910.1420867513011555No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCACCGC29690.14104164647714165No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAATCACCGC28760.13662370335744675No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCACCGC28460.13519856041560968No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCTGC25940.12322735970417834No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGTAGTCACCGC25910.12308484540999463No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGT25230.11985452140849727No Hit
CTTACCTGAGGAGACGATGACCAGGGTTCCCTGGCCCCAGTAGTCACCGC25020.11885692134921133No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAACGC24850.11804934034883698No Hit
CTTACCTGAGGAGACGGTGACCATGGTTCCCTGGCCCCAGTAGTCACCGC24800.11781181652519748No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAGTCACCGC24000.11401143534696531No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCACTGC23840.11325135911131887No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGA23740.11277631146403985No Hit
CTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGCC22220.1055555872253987No Hit
CTTACCTGAGGAGACGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGCC22160.10527055863703129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)