FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619070
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT14600323.58424733875006No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT500368.082446250020192No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT364585.889156315117838No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG285934.618702246918765No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC249934.037184809472273No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC195403.15634742436235No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG169542.738624065129953No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC152492.4632109454504336No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT118741.918038347844347No Hit
CTTACCTGAGGAGACGGTGACCTGGTGATCGACATCCCGGAGTGACCGTG78601.2696464050915082No Hit
CTTACCTGAGGAGACGGTGACCAGGGGCAAGGAGCCGGCTGAGCTCTCCT65851.0636923126625422No Hit
CTTACCTGAGGAGACGGTGACCTGGTGACCTGGCATGTGAGGCCCACGGG53680.8671071122813252No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT52820.8532153068312145No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT50920.8225241087437609No Hit
CTTACCTGAGGAGACGGTGACCCTTTCCCCCTCCTGGAGATGCTCTGCAT42760.6907134895892225No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC37020.5979937648408096No Hit
CTTACCTGAGGAGACGGTGACCAAGGGAGGCCTCCTTAACCTCCCAAGCC34180.5521185003311418No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTGCACTCGGGAGGGGAGAG31640.5110892144668616No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCAGCTGGT26330.42531539244350397No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCACTCGGGAGGGGAGAG25380.40996979339977707No Hit
CTTACCTGAGGAGACGGTGACCACTTGGGGACCAGAGCTCAGATCCAGAG24290.3923627376548694No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG23620.38154005201350416No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT22260.35957161548774774No Hit
CTTACCTGAGGAGACGGTGACCTGGGGCAGGGGAGGGGAGCAGGGCCGTG19120.3088503723326926No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCCTGGCCCCAGTGCTGGAAGT17920.2894664577511428No Hit
CTTACCTGAGGAGACGGTGACCCTGACTGTGCCCAGGGGGAGGGTTGGTG17520.2830051528906263No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTAGGTTGTA16280.26297510782302486No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC16220.26200591209394736No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT15890.2566753355840212No Hit
CTTACCTGAGGAGACGGTGACCTCACAGCCCTGCCCGCCAGCCGCCACAC13870.22404574603841243No Hit
CTTACCTGAGGAGACGGTGACCCATGGTGATCCCACTGGGGCAGGCACAG13800.22291501768782204No Hit
CTTACCTGAGGAGACGGTGACCCCATGTCTGATTGGTTCTCGGTGTTATT12500.20191577689114315No Hit
CTTACCTGAGGAGACGGTGANCGTGGTCCCTTTGCCCCAGACGTCCATGT12150.19626213513819118No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGACG10530.170093850453099No Hit
CTTACCTGAGGAGACGGTGACCCGTTTCCCTGAGGGACTCTTGTCGGTGG9960.16088649102686287No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT9520.15377905568029465No Hit
CTTACCTGAGGAGACGGTGANCAGGCCTGCTGTCCCCCAGGTAAGGACAG9360.15119453373608802No Hit
CTTACCTGAGGAGACGGTGACCCCAGAAGCATAGTCCAGGAGTTCCTTAG9260.14957920752095885No Hit
NTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG9130.14747928344129096No Hit
CTTACCTGAGGAGACGGTGACCCGAAGAGACAGCCTGAGGTCCGTCCTCA8980.14505629411859725No Hit
CTTACCTGAGGAGACGGTGACCCACTGTGCTGTGGGAGCCTCCATCCTTC8720.14085644595926147No Hit
CTTACCTGAGGAGACGGTGACCAGTGATGGCTCCCGCAGGAGCAGCGGGG8680.1402103154732098No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCGCTCGGGAGGGGAGAG8580.13859498925808067No Hit
CTTACCTGAGGAGACGGTGANCGTGGTCCCTTGGCCCCAGACGTCCATAC7820.12631851002309916No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT7540.12179559662073755No Hit
CTTACCTGAGGAGACGGTGACCCGATTTTCCAGGTGCCGTCTTTCACCCC7410.11969567254106966No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC6950.1122651719514756No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC6650.10741919330608815No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC6620.10693459544154943No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGGGGTCGAACC6510.10515773660490736No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC6480.10467313874036861No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)