FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445892
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT11281225.300296932889577No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT5452212.227624626591192No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC393178.817606057072117No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC307356.892924744108439No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG200904.505575341113992No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT146813.292501323190369No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT39910.8950597902631131No Hit
CTTACCTGAGGAGACGGTGACCTGGTGATCGACATCCCGGAGTGACCGTG38290.8587281225050012No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT38240.857606774734689No Hit
CTTACCTGAGGAGACGGTGACCCTTTCCCCCTCCTGGAGATGCTCTGCAT35890.8049034295300208No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCACTCGGGAGGGGAGAG35080.7867375956509649No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTGCACTCGGGAGGGGAGAG29290.6568855238488244No Hit
CTTACCTGAGGAGACGGTGACCCCAGGTCTGGGCCAGTGGAGCGATGAGT27930.6263848644963355No Hit
CTTACCTGAGGAGACGGTGACCTGGTGACCTGGCATGTGAGGCCCACGGG27050.6066491437388426No Hit
CTTACCTGAGGAGACGGTGACCCCATGTCTGATTGGTTCTCGGTGTTATT26770.6003695962250949No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCCTGGCCCCAGTGCTGGAAGT19570.4388955173001534No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT17800.3991998062311053No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC16050.359952634270182No Hit
CTTACCTGAGGAGACGGTGACCTGGGGCAGGGGAGGGGAGCAGGGCCGTG15780.35389735631049674No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG14680.32922770536363066No Hit
CTTACCTGAGGAGACGGTGACCCATGGTGATCCCACTGGGGCAGGCACAG14440.3238452360661326No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGGGGTCGAACC13810.30971625416020027No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC12320.27630009060489985No Hit
CTTACCTGAGGAGACGGTGANCCCACCTGCCTTTAATTAAAAGCTCAGTC11030.2473693181308478No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCGCTCGGGAGGGGAGAG10920.2449023530361612No Hit
CTTACCTGAGGAGACGGTGACCAAGGGAGGCCTCCTTAACCTCCCAAGCC10510.23570730131960205No Hit
CTTACCTGAGGAGACGGTGACCACTTGGGGACCAGAGCTCAGATCCAGAG10470.2348102231033524No Hit
CTTACCTGAGGAGACGGTGACCATGATGACCTGCAGGCATTGTTTTTTAG10210.2289792146977295No Hit
CTTACCTGAGGAGACGGTGACCTTCTTTTATTGCAGCTGTTTAGGAACAA10120.22696078871116773No Hit
CTTACCTGAGGAGACGGTGACCCACTGTGCTGTGGGAGCCTCCATCCTTC9950.22314820629210663No Hit
CTTACCTGAGGAGACGGTGACCTGATTAATTGGAACTCAGCCAAAAGCAG9510.21328034591336018No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC9180.20587945062930038No Hit
CTTACCTGAGGAGACGGTGACCCCAAGACACACAGTGACAAAGACACAGG9020.2022911377643017No Hit
CTTACCTGAGGAGACGGTGACCCTTGCCCTGAAAAGCTGCTATGTACATT8890.19937563356149024No Hit
NTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC8840.19825428579117815No Hit
CTTACCTGAGGAGACGGTGACCCCTCTGCAGCTGCTGGCCTGGTTGCTAA8690.19489024248024184No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTCATTAAAAGCTCAGTC8540.19152619916930558No Hit
CTTACCTGAGGAGACGGTGACCTGGGCTGGGTTTAGGACCTTGCTTGGCA7980.1789671041418101No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC6890.15452172274900647No Hit
CTTACCTGAGGAGACGGTGANCAGGCCTGCTGTCCCCCAGGTAAGGACAG6560.14712082746494667No Hit
NTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG6530.14644801880275943No Hit
CTTACCTGAGGAGACGGTGACCCCGGGTCAGACGGGCAGAGGGACAGCCC6460.14487813192432247No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTACTTAAAAGCTCAGTC6030.13523454109963848No Hit
CTTACCTGAGGAGACGGTGACCCGTTTCCCTGAGGGACTCTTGTCGGTGG5780.12962780224807802No Hit
CTTACCTGAGGAGACGGTGACCCACAGCTGGTCTCAAGGTCCACACTGCC5670.1271608371533914No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGATCGAAGT5390.12088128963964367No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT5060.11348039435558387No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT5020.1125833161393342No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT5000.11213477703120936No Hit
CTTACCTGAGGAGACGGTGACCCCCACACACATCAGTGCTCCCCACACAC4710.1056309599633992No Hit
CTTACCTGAGGAGACGGTGACCTCCCGCTGGCGCCGCCTTCGCCGCCAAC4680.10495815130121194No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCACGGCCCCAGAGATCGAAGT4610.10338826442277502No Hit
CTTACCTGAGGAGACGGTGACCATCCCTGAGTGTGTGCACATTCCACCCT4540.10181837754433809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)