FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382117
Sequences flagged as poor quality0
Sequence length300
%GC56

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC274827.192038040704811No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT265616.951012386258659No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC160954.2120607039205264No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG133113.48348804162076No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT96932.53665762057171No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG94572.474896432244574No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC79122.0705700086622687No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG58861.5403659088708432No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC53121.390150137261624No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGAC47421.2409811654545597No Hit
CTTACCTGAGGAGACGGTGACCATGCAGACACCACACGCTCTCCTGCAGT42241.1054205910755084No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC39781.0410424032429857No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTACCCCGGG39061.0222000068041988No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT37100.9709068164986117No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA35170.9203987260446408No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTAG24300.6359308798090637No Hit
CTTACCTGAGGAGACGGTGACCATTTTTAGCCCTTGGCTAGGTGTGGGGC23540.6160416835681218No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCTT23480.6144714838648896No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG21730.5686739925206155No Hit
CTTACCTGAGGAGACGGTGACCGGGCAGCACAAGGCAAGCAGCTCAGAGC21130.5529719954882929No Hit
CTTACCTGAGGAGACGGTGACCACCATCTTCATGGCCTGTTCCCATGAGG20000.5233999010774186No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTA19760.5171191022644896No Hit
CTTACCTGAGGAGACGGTGACCCCATCCACTTCTCAGGGAACAGGGCATT18120.47420031037614135No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGG16880.4417495165093414No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCA15700.4108689223457737No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC15010.3928116257586027No Hit
CTTACCTGAGGAGACGGTGACCCGCTTGCAGTATCTCCCAGTTTCACCAC14010.3666416307047318No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG13820.36166933164449633No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC12870.33680783634331896No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGTAG12300.3218909391626125No Hit
CTTACCTGAGGAGACGGTGACCATGCCACTGGACACCTCTGGTGCTGACT12020.3145633405475286No Hit
CTTACCTGAGGAGACGGTGACCTAACTGGTTTGTCCCCACTGTCTCCTCT11320.296244344009819No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC10640.27844874737318676No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTTGGAAGGACGGGCTGTATCTGG10330.27033604890648677No Hit
CTTACCTGAGGAGACGGTGACCTAGAGGCACAGGTCCGCCTCCAGCTCCT10140.2653637498462513No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCTACTTACTGGGT9920.25960635093439965No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTG9790.25620425157739646No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGGGGTCGAACC9710.2541106519730868No Hit
CTTACCTGAGGAGACGGTGACCCCGGGTGGTGAGACCTTAGGCTGTGAGC9070.2373618551386094No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC9040.23657675528699326No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC8970.23474485563322228No Hit
CTTACCTGAGGAGACGGTGACCTCTGTGGCTGCTGCACGGCACAGCCGAG8910.23317465592999No Hit
CTTACCTGAGGAGACGGTGACCCTGTCTGTAAAAAAAAGTTCTTAATTTT8900.23291295597945133No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT8840.23134275627621903No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT8750.2289874567213707No Hit
CTTACCTGAGGAGACGGTGACCCACAAGGGCTGGCCCCTGAATGCAGCAG8630.22584705731490615No Hit
CTTACCTGAGGAGACGGTGACCTCTGGCGGGTGTAGAAGAGCTCATGCTT8450.22113645820520939No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA8300.21721095894712875No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT8280.21668755904605133No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTACCAGC7880.20621956102450295No Hit
CTTACCTGAGGAGACGGTGACCAGACTCAAGTTACTGATCCTCCGCTGTT7630.19967706226103524No Hit
CTTACCTGAGGAGACGGTGACCCCTCTCTCTGGTTCCCAGCACCTGCCTG7590.1986302624588804No Hit
CTTACCTGAGGAGACGGTGACCATAGGGCCAGTTGGCTGAGCGGGCTGAG7500.196274962904032No Hit
CTTACCTGAGGAGACGGTGACCAAGGGTCCCATCCAGATGGGTGGCCGAG7300.1910409638932578No Hit
CTTACCTGAGGAGACGGTGACCACACACTGTCATGCGGGGAGCGTGTAGC7220.18894736428894815No Hit
CTTACCTGAGGAGACGGTGACCCCAGCACCCACAGCCTGCCTATGGGGCT7030.18397506522871268No Hit
CTTACCTGAGGAGACGGTGACCCAGTTGGCCAGTAGCCCACCCAGATCTT6820.17847936626739977No Hit
CTTACCTGAGGAGACGGTGACCAGAGAAGAGGGAAGCCCAAAGGGGCTGA6620.1732453672566256No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGTGACTCGG6520.1706283677512385No Hit
CTTACCTGAGGAGACGGTGACCTTAGGTGATGAGTTATTTAGGCTCTTTG6510.17036666780069978No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAATAAC6240.16330076913615463No Hit
CTTACCTGAGGAGACGGTGACCTGCTGCTGTGACTTCCTCTCCAGAGCTG5620.14707537220275466No Hit
CTTACCTGAGGAGACGGTGACCACGGAAGGAACCCCACCCTCTGTCCCCA5540.14498177259844497No Hit
CTTACCTGAGGAGACGGTGANCGTGGTCCCTTGGCCCCAGACGTCCATAC5470.14314987294467402No Hit
CTTACCTGAGGAGACGGTGACCCGGGCCAGAACGCCTGGCTGTCGTACCA5430.14210307314251916No Hit
CTTACCTGAGGAGACGGTGACCAGGGGAAGGCAGCAGGGAGTTCTGAGCA5430.14210307314251916No Hit
CTTACCTGAGGAGACGGTGACCTCATCTGTAAAAGGCAGTGAAAATTCCT5380.14079457338982562No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG5320.13922437368659338No Hit
CTTACCTGAGGAGACGGTGACCTCCGCCCCATCCAAGAAGGGCTGGGAGG5280.13817757388443855No Hit
CTTACCTGAGGAGACGGTGACCAGCACCCTCATGCTGACATCAGATGTCA5270.13791587393389984No Hit
CTTACCTGAGGAGACGGTGACCAGCCCAGGCAATGTAGCTGGTAAACGGC5220.13660737418120628No Hit
CTTACCTGAGGAGACGGTGACCCTGAGCATGATGCCAGAGTCTTATCTGG5160.13503717447797403No Hit
CTTACCTGAGGAGACGGTGACCGGGTGAAATCTATCAGACTGCAGGGAGA4890.12797127581342888No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC4860.12718617596181275No Hit
CTTACCTGAGGAGACGGTGACCGTGGTGGTAAGGGTGTCCAACCCGGCAG4830.1264010761101966No Hit
CTTACCTGAGGAGACGGTGANCATTGTCCCTTGGCCCCAGATATCAAAAG4780.12509257635750307No Hit
CTTACCTGAGGAGACGGTGACCCTGTCCTCATGTCCTGGCACCCGTGTTG4690.12273727680265469No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC4640.12142877704996115No Hit
CTTACCTGAGGAGACGGTGACCCTGGAAATGGGGCCGACCTGATGTGGAC4630.12116707709942244No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG4610.120643677198345No Hit
CTTACCTGAGGAGACGGTGACCATGGGAGGGGGCTGCAGAGAGTCTCAGA4590.12012027729726758No Hit
CTTACCTGAGGAGACGGTGACCCCCCCAAGCTATCCTGAGTAGGAGAGCA4510.11802667769295792No Hit
CTTACCTGAGGAGACGGTGACCAGCTCACATGTGCCAGGACCCAGTCAGG4490.1175032777918805No Hit
CTTACCTGAGGAGACGGTGACCAGGACCAGGATGGGATTTCATAGCAGGC4440.11619477803918696No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG4320.11305437863272244No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGGACAA4300.11253097873164503No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGTCTCTTCCAACCCCGAGGCTGT4300.11253097873164503No Hit
CTTACCTGAGGAGACGGTGACCTGGCGTCCCCCAAGGACGCCCTACGGAG4250.11122247897895148No Hit
CTTACCTGAGGAGACGGTGACCTCTCTCCAGGCTCTAAGGGAGAGCGGGT4170.10912887937464179No Hit
CTTACCTGAGGAGACGGTGACCAGAGAAGCGGAAGCCTGAGGGGGTTGAG4160.10886717942410308No Hit
CTTACCTGAGGAGACGGTGACCCGGCCCAGCTAGGAGCAGGTGGGGGGCC4140.10834377952302567No Hit
CTTACCTGAGGAGACGGTGACCACCATCATCGCTCCGCCGGGCCACACAG4140.10834377952302567No Hit
CTTACCTGAGGAGACGGTGACCTTTCCTGTGGGGGCCATCTGTCATCCTG4100.10729697972087084No Hit
CTTACCTGAGGAGACGGTGACCCTTCTGCGGGGCTCTGGACAAGCAGCAG4090.10703527977033213No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG4080.10677357981979342No Hit
CTTACCTGAGGAGACGGTGACCCATCCTGTTTGCAACATGTGCATGGCAA4080.10677357981979342No Hit
CTTACCTGAGGAGACGGTGACCACACGGCGCAGCTGCTTTGCACCCGGGA3970.1038948803638676No Hit
CTTACCTGAGGAGACGGTGACCTACTTTGTGGGGTGGTTAAAAAAAAAAA3950.10337148046279018No Hit
CTTACCTGAGGAGACGGTGACCGGCTGTGCGGGAGCTGTGACACGGTGCC3920.10258638061117406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)