FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358950
Sequences flagged as poor quality0
Sequence length300
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG5356414.922412592283047No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG101522.8282490597576264No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT98482.7435575985513303No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT55101.5350327343641175No Hit
CTTACCTGAGGAGACGGTGACCGGGCAGCACAAGGCAAGCAGCTCAGAGC46791.3035241677113805No Hit
CTTACCTGAGGAGACGGTGACCATGCAGACACCACACGCTCTCCTGCAGT42831.193202395876863No Hit
CTTACCTGAGGAGACGGTGACCCCATCCACTTCTCAGGGAACAGGGCATT35810.9976319821702186No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG34960.9739518038724057No Hit
CTTACCTGAGGAGACGGTGACCGACGTGCTGTAGACGCGCAGGCCGCTCA28340.7895250034823791No Hit
CTTACCTGAGGAGACGGTGACCACCATCTTCATGGCCTGTTCCCATGAGG21180.5900543251149185No Hit
CTTACCTGAGGAGACGGTGACCATGCCACTGGACACCTCTGGTGCTGACT20480.5705530018108372No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTTGGAAGGACGGGCTGTATCTGG19660.5477085945117705No Hit
CTTACCTGAGGAGACGGTGACCAGACTCAAGTTACTGATCCTCCGCTGTT18130.5050842735757068No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC17690.4928262989274272No Hit
CTTACCTGAGGAGACGGTGANCATTGTCCCTTGGCCCCAGATATCAAAAG17310.48223986627664017No Hit
CTTACCTGAGGAGACGGTGACCTAGAGGCACAGGTCCGCCTCCAGCTCCT16800.4680317592979524No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGG16590.46218136230672796No Hit
CTTACCTGAGGAGACGGTGACCTAACTGGTTTGTCCCCACTGTCTCCTCT16540.46078841064215076No Hit
CTTACCTGAGGAGACGGTGACCCACAAGGGCTGGCCCCTGAATGCAGCAG16500.45967404931048894No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG16430.4577239169800808No Hit
CTTACCTGAGGAGACGGTGACCCCGGGTGGTGAGACCTTAGGCTGTGAGC15900.442958629335562No Hit
CTTACCTGAGGAGACGGTGACCCGCTTGCAGTATCTCCCAGTTTCACCAC15370.42819334169104334No Hit
CTTACCTGAGGAGACGGTGACCACACGGCGCAGCTGCTTTGCACCCGGGA14590.40646329572363843No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG13970.3891906950828806No Hit
CTTACCTGAGGAGACGGTGACCATAGGGCCAGTTGGCTGAGCGGGCTGAG13450.37470399777127733No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC13390.37303245577378463No Hit
CTTACCTGAGGAGACGGTGACCATTTTTAGCCCTTGGCTAGGTGTGGGGC12560.34990945814180247No Hit
CTTACCTGAGGAGACGGTGACCTCTGGCGGGTGTAGAAGAGCTCATGCTT11320.31536425686028696No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC11160.3109068115336398No Hit
CTTACCTGAGGAGACGGTGACCAGGACCAGGATGGGATTTCATAGCAGGC11130.3100710405348934No Hit
CTTACCTGAGGAGACGGTGACCACGGAAGGAACCCCACCCTCTGTCCCCA11130.3100710405348934No Hit
CTTACCTGAGGAGACGGTGACCCATCCTGTTTGCAACATGTGCATGGCAA11060.30812090820448534No Hit
CTTACCTGAGGAGACGGTGACCCTGTCTGTAAAAAAAAGTTCTTAATTTT10850.3022705112132609No Hit
CTTACCTGAGGAGACGGTGACCACGATTGTATGACAATAAAATGGACCTT10800.30087755954868367No Hit
CTTACCTGAGGAGACGGTGACCAAGGGTCCCATCCAGATGGGTGGCCGAG10670.29725588522078283No Hit
CTTACCTGAGGAGACGGTGACCCCAGCACCCACAGCCTGCCTATGGGGCT10450.291126897896643No Hit
CTTACCTGAGGAGACGGTGACCAGAGAAGAGGGAAGCCCAAAGGGGCTGA10200.2841621395737568No Hit
CTTACCTGAGGAGACGGTGACCGCCGCGGTGGAGGAGCTTTTGGTGGCCG10130.28221200724334866No Hit
CTTACCTGAGGAGACGGTGACCACGGTAGGAGGAAGTAGCCCCTCTACTA9860.27469006825463155No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGTGACTCGG9800.27301852625713885No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAATCCCGCACCGT9750.27162557459256165No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG9500.26466081626967547No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG9470.2638250452709291No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC9030.2515670706226494No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT8550.2381947346427079No Hit
CTTACCTGAGGAGACGGTGACCACACACTGTCATGCGGGGAGCGTGTAGC8550.2381947346427079No Hit
CTTACCTGAGGAGACGGTGACCACCATCATCGCTCCGCCGGGCCACACAG8350.23262292798439896No Hit
CTTACCTGAGGAGACGGTGACCAAGAAAAGGGCCAGAGGCCGCAGGCTGA7980.22231508566652736No Hit
CTTACCTGAGGAGACGGTGACCGGGTGAAATCTATCAGACTGCAGGGAGA7980.22231508566652736No Hit
CTTACCTGAGGAGACGGTGACCTCTGTGGCTGCTGCACGGCACAGCCGAG7320.20392812369410784No Hit
CTTACCTGAGGAGACGGTGACCCAGTTGGCCAGTAGCCCACCCAGATCTT7210.20086363003203792No Hit
CTTACCTGAGGAGACGGTGACCATGGGAGGGGGCTGCAGAGAGTCTCAGA7100.19779913636996793No Hit
CTTACCTGAGGAGACGGTGACCACCTCTGAACCCGCAATGTCCCTTGCTG6920.1927845103774899No Hit
CTTACCTGAGGAGACGGTGACCTGCTGCTGTGACTTCCTCTCCAGAGCTG6600.18386961972419558No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG6400.1782978130658866No Hit
CTTACCTGAGGAGACGGTGACCTCGGGGACCGTTCTCTGATCATCAGGAT6370.17746204206714025No Hit
CTTACCTGAGGAGACGGTGACCCCTGTCCTTCTCACGGGGGGTCTCCAGC6140.17105446441008498No Hit
CTTACCTGAGGAGACGGTGACCCCTCTCTCTGGTTCCCAGCACCTGCCTG6130.17077587407716951No Hit
CTTACCTGAGGAGACGGTGACCGTGGTGGTAAGGGTGTCCAACCCGGCAG5900.16436829642011422No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT5720.15935367042763615No Hit
CTTACCTGAGGAGACGGTGACCTCCGCCCCATCCAAGAAGGGCTGGGAGG5510.15350327343641176No Hit
CTTACCTGAGGAGACGGTGACCCCACAAATAACTCGCTGAGGTCTGGGGC5490.15294609277058085No Hit
CTTACCTGAGGAGACGGTGACCAGCTCACATGTGCCAGGACCCAGTCAGG5480.15266750243766541No Hit
CTTACCTGAGGAGACGGTGACCTGAGCACCCCCTGGTGTTCTGAGCTTCC5370.1496030087755955No Hit
CTTACCTGAGGAGACGGTGACCGCTGTGGATTGGGGTCATGGCCGCCGGG5360.14932441844268005No Hit
CTTACCTGAGGAGACGGTGACCTCTCTCCAGGCTCTAAGGGAGAGCGGGT5330.1484886474439337No Hit
CTTACCTGAGGAGACGGTGACCCAGGGACACCACACTCGGTGTCAGCCTC5020.1398523471235548No Hit
CTTACCTGAGGAGACGGTGACCAGCCCAGGCAATGTAGCTGGTAAACGGC5020.1398523471235548No Hit
CTTACCTGAGGAGACGGTGACCCCAGCAGTGTGGCCTGTGGGAGTCCAGA4990.13901657612480847No Hit
CTTACCTGAGGAGACGGTGACCCACCCGGCTTGGGCCCTAGGCCAGCCAG4980.138737985791893No Hit
CTTACCTGAGGAGACGGTGACCTTTCCTGTGGGGGCCATCTGTCATCCTG4940.13762362446023121No Hit
CTTACCTGAGGAGACGGTGACCCCTAGTGGGCAGTTTGCAAAATGTGATT4880.13595208246273854No Hit
CTTACCTGAGGAGACGGTGACCCGAGCCTCCTGTGGCCCTGGCCCCTGGT4510.12564424014486697No Hit
CTTACCTGAGGAGACGGTGACCCTGTCCTCATGTCCTGGCACCCGTGTTG4440.12369410781445883No Hit
CTTACCTGAGGAGACGGTGACCCCCCCAAGCTATCCTGAGTAGGAGAGCA4410.1228583368157125No Hit
CTTACCTGAGGAGACGGTGACCTCATTCACTGGGCCCATTCCCAGGGATG4330.12062961415238892No Hit
CTTACCTGAGGAGACGGTGACCCCCTCCCCTTCCTCAGACTTATATAGGA4330.12062961415238892No Hit
CTTACCTGAGGAGACGGTGACCCGTAAAAAATACGAACGGATCGTCCAGC4190.11672934949157265No Hit
CTTACCTGAGGAGACGGTGACCGCGACAACATCTCCCATCCACGGGCTCT4150.11561498815991086No Hit
CTTACCTGAGGAGACGGTGACCCACAGGATGGCCCCAGCCATAGTGACTC4110.11450062682824907No Hit
CTTACCTGAGGAGACGGTGACCCCTCCCTCGTGGCTCAGTAAGTGTGGCC4060.11310767516367182No Hit
CTTACCTGAGGAGACGGTGACCTTGCGCACCTTCTCCAGCAGCTTGCTCA3850.10725727817244742No Hit
CTTACCTGAGGAGACGGTGACCCACGAGGGAGCAGGGTGACACCCATTGG3830.10670009750661652No Hit
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG3820.10642150717370108No Hit
CTTACCTGAGGAGACGGTGACCCTGCTGCCTACCTCTGTCTGAAGTTGGG3790.10558573617495473No Hit
CTTACCTGAGGAGACGGTGACCAGGAGGGGAGCTGCAGGGAGGGCATCCC3780.10530714584203929No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT3760.10474996517620838No Hit
CTTACCTGAGGAGACGGTGACCTCGGACCTCCTTGCTTCCGACCAGATCC3720.10363560384454659No Hit
CTTACCTGAGGAGACGGTGACCTTAGGTGATGAGTTATTTAGGCTCTTTG3710.10335701351163115No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT3680.1025212425128848No Hit
CTTACCTGAGGAGACGGTGACCCTGGACTCCCACTGTGTGTGCCAGCGGA3670.10224265217996936No Hit
CTTACCTGAGGAGACGGTGACCATCCCTGAGTGTGTGCACATTCCACCCT3670.10224265217996936No Hit
CTTACCTGAGGAGACGGTGACCCTGCCTAGGATGTCCTATCTTAAGGTGA3660.10196406184705392No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG3660.10196406184705392No Hit
CTTACCTGAGGAGACGGTGACCCTTCTGCGGGGCTCTGGACAAGCAGCAG3620.10084970051539213No Hit
CTTACCTGAGGAGACGGTGACCTTGGCAGTCGTGGAAGGCCAAGGCCTGG3620.10084970051539213No Hit
CTTACCTGAGGAGACGGTGACCTGCTGGAGCGTCGTGCCAGCATGGGCTG3600.10029251984956122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)