FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001132498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796758
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC1357084.8523325936673825No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT1236634.421655359526995No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC658142.3532246980253566No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC573782.051589733541479No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG361981.294284310619653No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC330691.1824047700945166No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT303631.0856498846164024No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAATAAC218720.782048357419555No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC180130.6440671663404557No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG176880.6324465684910886No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT120350.4303196772834832No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC119670.4278882906565387No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT110260.39424219042190994No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAAC101770.3638856132707943No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT93150.33306421220570387No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA86610.3096799937642084No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC81520.29148034974781517No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC69830.24968195317578426No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT67780.24235203760926044No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC65060.2326264911014825No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG64450.23044539427437052No Hit
CTTACCCGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC60020.21460562551354107No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCGAACC57030.2039146754921234No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG56220.2010184649512042No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAACC53640.19179349804309131No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC53210.19025600355840586No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC48940.17498832576862208No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAACC47980.17155577994234752No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT41480.1483145842436135No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC41310.14770673758687736No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA39550.141413736905374No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC38560.13787392402202836No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCAAACC36520.13057976414119493No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGT36300.129793139056007No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC36000.12872046848529617No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT35310.12625332617266133No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC35020.1252164112876409No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATACC34380.12292804740345786No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG34180.12221293368965065No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT34110.12196264388981815No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG32490.1161702228079798No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAACC29910.10694525589986692No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC29810.10658769904296332No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC29650.10601560807191755No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA29090.10401328967325739No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG28910.1033696873308309No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAT28710.10265457361702372No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGT28280.10111707913233822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)