FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562497
Sequences flagged as poor quality0
Sequence length300
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT7529813.38638250515114No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG468018.320222152295925No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT455728.101732098126746No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC422957.519151213250915No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG99981.7774317018579653No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG80791.4362743267964095No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT66351.1795618465520705No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA50120.8910269743660856No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC46470.8261377394012769No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT40810.7255149805243406No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC38660.6872925544491792No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG34410.6117365959285116No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC33870.6021365447282385No Hit
CTTACCCGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT32950.5857809019425881No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA32850.5840031146832783No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT30550.5431140077191522No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT27470.4883581601324096No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC24010.4268467209602895No Hit
CTTACCCGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG20710.36817974140306525No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG19450.34577962193576145No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT19040.33849069417259114No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG18800.33422400475024755No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG18590.3304906515056969No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC18580.3303128727797659No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC17720.3150239023497014No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG17500.3111127703792198No Hit
CTTACCCGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGTAGTCAAAGT17190.3056016298753593No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT16240.288712650911916No Hit
CTTACCCGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGGGGTCGAACC15470.27502368901523033No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC15250.2711125570447487No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA14050.24977910993303076No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC11630.2067566582577329No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT11470.20391219864283722No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTTGCCCCAGACGTCCATGT11420.2030233050131823No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT10750.19111213037580646No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAA10500.18666766222753187No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA10390.18471209624229107No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT10360.18417876006449813No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGA9710.17262314287898423No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG9610.17084535561967443No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTCTGCCCCAGACGTCCATGT8730.15520082773774793No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC8140.14471188290781997No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC7540.13404515935196099No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG7290.12960069120368642No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGT7000.12444510815168791No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA6930.12320065707017104No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG6810.12106731235899924No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAT6470.11502283567734584No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAC6430.1143117207736219No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAT6420.11413394204769092No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG6410.11395616332175994No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAT6170.10968947389941636No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAT5970.10613389938079669No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG5810.10328943976590098No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC5800.10311166103996999No Hit
NTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG5790.10293388231403901No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAA5730.10186720995845311No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAAAAG5710.10151165250659115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)