FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256556
Sequences flagged as poor quality0
Sequence length300
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT23139718.415176084472158No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC13536410.772619763862494No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC726775.783824994667965No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG353852.8160304833210774No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG347622.7664505203110723No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG291892.3229366617962115No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC135861.0812092736018133No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG128911.0258993630208284No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT94980.7558755837384088No Hit
CTTACCCGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGTAGTCAAAGT94770.7542043490302064No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG78680.62615593733984No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG78260.6228134679234352No Hit
CTTACCTGAGGAGACGGTGACCGCCGCGGTGGAGGAGCTTTTGGTGGCCG72850.5797592785359348No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT71470.5687768790248903No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG67970.5409229672215166No Hit
CTTACCTGAGGAGACGGTGACCACCAGACCACAGGCTCAGAAACGAGGTT67540.5375009151999592No Hit
CTTACCCGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC63510.5054291253235033No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT62880.500415421198896No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGATGACC62510.4974708648082537No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC58920.46890070955850754No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGGACAA58850.46834363132244006No Hit
CTTACCTGAGGAGACGGTGACCTGAAACTGCGTGGATCTGCTGTTTCCAT58000.46157910988447787No Hit
NTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC56660.4509150407940434No Hit
CTTACCTGAGGAGACGGTGACCGTGGGCAGGGGCTTCCAAGCCAACAGGG55670.44303636288394627No Hit
CTTACCTGAGGAGACGGTGACCAGGGCATCTGAGCACCGCGGGACCTGCC55360.4405693021242189No Hit
CTTACCTGAGGAGACGGTGACCCGCTTGCAGTATCTCCCAGTTTCACCAC50560.40236965165102073No Hit
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG48000.3819965047319817No Hit
CTTACCTGAGGAGACGGTGACCCGGCCAGGGCTGGGGGCTGGGGGTGAGT46780.3722874269033771No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC43930.34960638443491576No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC39540.31466962077296995No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA37070.2950127173003034No Hit
CTTACCTGAGGAGACGGTGACCTCCTAGGCCTAGGCCGACTCCTTCATCC31490.2506056236252105No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC31310.24917313673246555No Hit
CTTACCCGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC30890.2458306673160607No Hit
CTTACCTGAGGAGACGGTGACCAAGCCATCAGGGACCGGTTCTCCCGGCT30140.23986197192962352No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGTCTCTTCCAACCCCGAGGCTGT29480.23460951998955878No Hit
CTTACCTGAGGAGACGGTGACCCCCTCCCCTTCCTCAGACTTATATAGGA28260.22490044216095423No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC28170.22418419871458173No Hit
CTTACCTGAGGAGACGGTGACCATGCCACTGGACACCTCTGGTGCTGACT27550.21925007719512699No Hit
CTTACCTGAGGAGACGGTGACCCCAGCACCCACAGCCTGCCTATGGGGCT27150.21606677298902718No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAAAACCGCACCGT24400.19418155657209069No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT22720.18081167890647135No Hit
CTTACCTGAGGAGACGGTGACCTCACGGGACAGCCTTTGCCGTGTGGTTT21590.17181884452423926No Hit
CTTACCTGAGGAGACGGTGACCTGCACCGTAGTCCACCCCCTCCCATTAC20670.1644972448502096No Hit
CTTACCTGAGGAGACGGTGACCTTAGGTGATGAGTTATTTAGGCTCTTTG20550.16354225358837965No Hit
CTTACCTGAGGAGACGGTGACCCTTCTGCGGGGCTCTGGACAAGCAGCAG20440.16266684493170222No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA19880.15821021904316243No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT19850.15797147122770494No Hit
CTTACCTGAGGAGACGGTGACCTCTCTCCAGGCTCTAAGGGAGAGCGGGT19620.15614107130919752No Hit
CTTACCTGAGGAGACGGTGACCCCTGTCCTTCTCACGGGGGGTCTCCAGC18300.14563616742906801No Hit
CTTACCTGAGGAGACGGTGACCCTGCCTAGGATGTCCTATCTTAAGGTGA17900.14245286322296818No Hit
CTTACCTGAGGAGACGGTGACCCAGGGACACCACACTCGGTGTCAGCCTC17680.14070204590961324No Hit
CTTACCTGAGGAGACGGTGACCCGGCAGCAGCACATGGCACCCGCGCAGA17290.1375983243086659No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG16760.13338044623558362No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCGG16620.13226628976344865No Hit
CTTACCTGAGGAGACGGTGACCACAGGCGACTGTGACAGTGACGGTGACC15940.12685467261307892No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG15840.12605884656155397No Hit
CTTACCCGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG15760.125422185720334No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAATCCCGCACCGT15380.12239804672453915No Hit
CTTACCCGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG15260.1214430554627092No Hit
CTTACCTGAGGAGACGGTGACCTTTCCTGTGGGGGCCATCTGTCATCCTG15140.12048806420087922No Hit
CTTACCTGAGGAGACGGTGACCCCACACCTCGGGAACGCTGCACCTTCTG14810.11786183823084685No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG14310.11388270797322204No Hit
NTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG14300.11380312536806954No Hit
CTTACCCGAGGAGACGGTGACCCGCACCAAGCTCAGAGAGGGCAGGGGTG14160.1126889688959346No Hit
CTTACCTGAGGAGACGGTGACCAGGACACTCACCCAGATCTGGGGTCGGC14140.1125298036856296No Hit
NTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG13910.1106994037671222No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAAGGGTG13520.10759568216617485No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG13100.10425321274977No Hit
CTTACCTGAGGAGACGGTGACCCAAGGCGACGGAGGAAAGCCGTGTGGGG13080.10409404753946501No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG12790.10178615199004262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)