FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417207
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC16220338.878302617166064No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT14416234.554070281658746No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT107412.5745013866018547No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT77471.8568720083795336No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG36330.8707907585443196No Hit
CTTACCTGAGGAGACGGTGACCACTAATGGGATTAGCCACTGTCCTTGAT35170.8429868146986987No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT33720.8082318848916725No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC28680.687428542665871No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC17860.4280848595541302No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGACGTCCATAC9690.23225880677936853No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC9290.22267123993605092No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC8280.19846263365667405No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCCTGGCCCCAGACGTCCATAC8060.19318947189284935No Hit
CTTACCTGAGGAGACGGTGACCAAGGGAGGCCTCCTTAACCTCCCAAGCC7810.18719724261577586No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTCGAAGT7610.18240345919411707No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCAATAC7540.1807256349965365No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC7380.17689060825920946No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC7220.1730555815218824No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTGGCCCCAGACGTCCATAC6360.15244231280874962No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC6300.15100417778225197No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTGCACTCGGGAGGGGAGAG5810.13925940839918793No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGGTGGAAGT5800.139019719228105No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGCTGGAAGT5280.12655588233179213No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT4980.11936520719930395No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTCGAAGT4930.11816676134388926No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC4520.10833950532948872No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGCAGT4400.10546323527649344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)