FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001132506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102267
Sequences flagged as poor quality0
Sequence length300
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT4260041.65566605063217No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC84358.248017444532449No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC24882.4328473505627426No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG24012.3477759198959585No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG11271.1020172685225929No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG10421.0189015029286086No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC10411.0179236703922088No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCACGGCCCCAGAGATCGAAGT9650.9436083976258227No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA9260.9054729287062299No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG6800.6649261247518751No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCATGGCCCCAGAGATCGAAGT6710.6561256319242766No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG5620.5495418854566967No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGCCTTTTA5350.5231404069739017No Hit
CTTACCTGAGGAGACGGTGACCGCCGCGGTGGAGGAGCTTTTGGTGGCCG4830.4722931150811112No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCCGGCCCCAGAGATCGAAGT4560.44589163659831615No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCAAACC4560.44589163659831615No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC4330.4234014882611204No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTACCCCT4100.40091133992392464No Hit
CTTACCTGAGGAGACGGTGACCACCAGACCACAGGCTCAGAAACGAGGTT3840.3754876939775294No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA3680.35984237339513236No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG3640.35593104324953306No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAATAG3580.3500640480311342No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGGGGTCGAACC3420.33441872744873713No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG3410.3334408949123373No Hit
CTTACCTGAGGAGACGGTGACCACCTTTCCCTGGCCCCAATATTCATAAT3360.32855173223033823No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTACCACC3080.3011724212111434No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCAATCG2970.29041626331074544No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGGTCGAAGT2760.2698817800463492No Hit
CTTACCTGAGGAGACGGTGACCAGGACCAGGATGGGATTTCATAGCAGGC2750.26890394750994945No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCCC2710.2649926173643502No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTCGGCCCCAGACGTCCGCAC2430.23761330634515535No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCATAAA2430.23761330634515535No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC2250.22001232068995866No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGATCGAAGT2160.2112118278623603No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGC2100.2053448326439614No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGACG2080.20338916757116177No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT1980.1936108422071636No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG1910.18676601445236488No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGAAGTCCCCCC1900.18578818191596508No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGCTCGAAGT1770.17307635894276746No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGGACAA1770.17307635894276746No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG1760.17209852640636764No Hit
CTTACCTGAGGAGACGGTGACCACACACTGTCATGCGGGGAGCGTGTAGC1760.17209852640636764No Hit
CTTACCTGAGGAGACGGTGACCCTGAGCATGATGCCAGAGTCTTATCTGG1750.17112069386996784No Hit
CTTACCTGAGGAGACGGTGACCAGGGCATCTGAGCACCGCGGGACCTGCC1710.16720936372436856No Hit
CTTACCTGAGGAGACGGTGACCACACGCAGTGTGATATCCTGGGACAGAA1680.16427586611516912No Hit
CTTACCTGAGGAGACGGTGACCCTGCCTAGGATGTCCTATCTTAAGGTGA1560.15254187567837132No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC1500.14667488045997243No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCATCTA1410.13787438763237408No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGCAGT1400.13689655509597426No Hit
CTTACCTGAGGAGACGGTGACCAGACTCAAGTTACTGATCCTCCGCTGTT1320.12907389480477574No Hit
CTTACCTGAGGAGACGGTGACCTGAGCACCCCCTGGTGTCCTGAGCTTCC1310.12809606226837592No Hit
CTTACCTGAGGAGACGGTGACCCTTCTGCGGGGCTCTGGACAAGCAGCAG1280.12516256465917647No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGAACAA1250.12222906704997703No Hit
CTTACCTGAGGAGACGGTGACCAGCCCAGGCAATGTAGCTGGTAAACGGC1230.12027340197717738No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGCGACC1220.11929556944077757No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGAGATCGAAGT1220.11929556944077757No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT1200.11733990436797793No Hit
CTTACCTGAGGAGACGGTGACCAGCACCCTCATGCTGACATCAGATGTCA1170.1144064067587785No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT1100.10756157900397978No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCACGGCCCCAGAGGTCGAAGT1100.10756157900397978No Hit
CTTACCTGAGGAGACGGTGACCAGGGAAGTCAGAGGCCTTGCTGGGTGCC1090.10658374646757995No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTTGGAAGGACGGGCTGTATCTGG1070.10462808139478033No Hit
CTTACCTGAGGAGACGGTGACCAGGGCGCCACGGCCCCAGAGATCGAAGT1070.10462808139478033No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCAAAGT1050.1026724163219807No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACTGCCCCAGAGATCGAAGT1040.10169458378558088No Hit
CTTACCTGAGGAGACGGTGACCTTGTGGCAGCCCCTCTGGCCCCTTGGCC1040.10169458378558088No Hit
CTTACCTGAGGAGACGGTGACCATCCAGCCCAGAATCTTCTAGTCAGAGC1040.10169458378558088No Hit
CTTACCTGAGGAGACGGTGACCATGCCACTGGACACCTCTGGTGCTGACT1040.10169458378558088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)