FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339968
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC13196038.815417921686745No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG12003635.308028990963855No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT294798.671110222138555No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT14600.4294521837349397No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC11100.3265013177710843No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGG9600.28237951807228917No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG9510.2797322100903615No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC9280.2729668674698795No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTCCCTCGCT7970.23443382906626506No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC7290.2144319465361446No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT7240.21296121987951808No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC6720.1976656626506024No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAACCCAGAT6590.1938417733433735No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC6370.18737057605421686No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCAATAC6160.18119352409638553No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCAAAGG5990.17619305346385541No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTAGCCCCAGACGTCCATAC5970.17560476280120482No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGG5770.16972185617469882No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAAGG5560.16354480421686746No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA5200.15295557228915663No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTGGCCCCAGACGTCCATAC5080.14942582831325302No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAATGCTGGAAGT4820.14177804969879518No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG4590.13501270707831325No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGG4430.13030638177710843No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC4300.1264824924698795No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACAGG4190.1232468938253012No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCCTGGCCCCAGACGTCCATAC3820.11236351656626507No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGG3760.11059864457831327No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG3630.10677475527108435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)