FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366954
Sequences flagged as poor quality0
Sequence length300
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT18436650.24226469802755No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGTAGC10713329.195212478948314No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT45841.2492028973658824No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGAAGC28580.7788442148062155No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTAGC28560.7782991873640838No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT22550.6145184410035045No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC22140.6033453784398044No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC13650.37198122925489296No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGC13000.35426783738561235No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTCGAAGT10330.2815066738610289No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTAGTAGTAGC10250.27932656409250206No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC9510.25916054873362876No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT8630.23517934127983345No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGCTGGAAGT8460.2305466080217139No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGCAGT7860.21419578475776255No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGCAGC7090.19321222823569165No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTCGAAGT6250.17032107566615978No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGTACC6190.16868599333976464No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG5400.14715740937556207No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTTGCCCCAGTGCTGGAAGT5340.14552232704916693No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGGTGGAAGT5280.14388724472277178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGGGCTGGAAGT4840.13189664099587414No Hit
CTTACCTGAGGAGACGGTGCCCAGGGTGCCCTGGCCCCAGTGCTGGAAGT4570.12453877052709605No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT4430.12072357843217407No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGG4350.1185434686636472No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGAAAGT4240.1155458177319228No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGA3930.10709789237888127No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAATAGC3840.10464526888928857No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGTAAC3750.10219264539969587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)