FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001132518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837923
Sequences flagged as poor quality0
Sequence length300
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC24790829.58601207986891No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC781869.330928975574128No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT694448.287635021356378No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG462325.51745208091913No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTA192882.301882153849459No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT171042.0412376793571725No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGA144771.7277243851762036No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACA133621.5946572656437408No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGCTGC133211.5897642146116049No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT123171.469944135678338No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGTAC116511.3904618920831628No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGAGGATAGC115631.3799597337702867No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTA114541.3669513785872927No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCTGC107841.286991764159714No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGCCTAGAC106351.2692097006526852No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCCGTATAATACC104421.2461765579892186No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGT101501.2113284872237664No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT98731.178270557079827No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG95321.1375746936174327No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG94391.1264758217640523No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC87801.047828977125583No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAG82740.9874415668265462No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGAGACCGA80140.9564124627203215No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTTTTGGG79390.9474617596127568No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGAGACGT78160.9327826065163506No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA77750.9278895554842151No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC68040.8120077859182765No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACTATCTGTTCG67650.8073534203023428No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGT58150.6939778476065224No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCAGAGGCCTCATAA46710.5574497895391343No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTAAC43890.5237951458546908No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCAT42110.502552143812737No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCA32570.388699200284513No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC19620.23415039329389453No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC13390.15979988614705648No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC11130.13282843411626127No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC10680.12745801225172243No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCAATAC10320.12316167476009134No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTGGCCCCAGACGTCCATAC9890.11802993831175419No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTAGCCCCAGTAGTCAAGTA8650.10323144250724708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)