FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644116
Sequences flagged as poor quality0
Sequence length84-300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC49176776.34758335455103No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT6869410.66484918865546No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTTGCCCCAGGGGTCGAACC27830.4320650317644648No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGGGGTCGAACC26530.41188233175390765No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC19160.29746194784790314No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAC17870.2774344993758888No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCGAACC15690.24358966397356996No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC15310.2376901055089456No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCGTCGAACC13700.21269460780356333No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGTCGAACC13580.21083158934105034No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC11600.18009178470958648No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGCCGAACC11070.17186345316682086No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT10800.1676716616261667No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT9600.1490414770010371No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCAAACC9560.1484204708468661No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGGGGTCGAACC8740.13568984468636083No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGGGGTCGAACC7840.12171720621751363No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGATCC7080.10991808928826484No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTGTCGAACC6970.10821032236429462No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGGGTCGAACC6750.10479478851635418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)