FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001132539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752610
Sequences flagged as poor quality0
Sequence length124-300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT61943082.30424788403025No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC277113.681986686331566No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC107661.430488566455402No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT81081.0773176014137469No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCAATGT36720.48790210068960016No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTTGCCCCAGACGTCCATGT27950.3713742841577975No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGT16130.21432083017764847No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGG12180.16183680790847849No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC10930.14522794010177914No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTTGCCCCAGACGTCCATGT10160.13499687753285233No Hit
CTTACCTGAGGAGACGGTGACCGTGGCCCCTTTGCCCCAGACGTCCATGT9450.12556304061864712No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC8830.11732504218652422No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTTGCCCCAGACGTCCATGT8370.11121297883365885No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCGATGT7940.1054995283081543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)