FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001132540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112346
Sequences flagged as poor quality0
Sequence length84-300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC2132318.979758958930447No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC1782015.861712922578464No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG67666.022466309436918No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT63465.648621223719581No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT47984.270735050647108No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG13421.194524059601588No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC8770.7806241432716785No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG7460.6640200808217471No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG7410.6595695440870168No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT6420.5714489167393587No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA5240.46641624979972585No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC5230.46552614245277985No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG4270.38007583714595977No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG3780.33646057714560373No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC3740.3329001477578196No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC3740.3329001477578196No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA3580.31865843020668294No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC3570.3177683228597369No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC3510.31242767877806066No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAAAAG3280.2919552097983017No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGGT3040.2705926334715967No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT2830.25190037918572983No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTA2730.2429993057162694No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTACC2720.24210919836932335No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCC2610.23231801755291687No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCACATATCAAAAG2560.22786748081818664No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC2490.22163672938956439No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC2440.21718619265483416No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCAAA2420.21540597796094207No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCCG2410.21451587061399607No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAG2370.2109554412262119No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG2350.2091752265323198No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC2310.20561479714453565No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGCCGAACC2250.20027415306285937No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG2150.19137307959339897No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAC2120.18870275755256083No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCCAGTG2110.1878126502056148No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTCCCG2110.1878126502056148No Hit
CTTACCTGAGGAGACGGTGACCACGGTTCCCTGGCCCCAGTGTTCCATGT2080.1851423281647767No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAAACGTCCAAAG2040.1815818987769925No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCTCC2020.1798016840831004No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGGGGTCGAACC2010.17891157673615438No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGT1990.17713136204226232No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCGGAGT1910.17001050326669395No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGA1910.17001050326669395No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGTC1900.16912039591974792No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGATT1900.16912039591974792No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTG1880.16734018122585584No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT1880.16734018122585584No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG1870.1664500738789098No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTA1850.16466985918501773No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACCAAAT1850.16466985918501773No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATATA1840.16377975183807167No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCGGGCCCCAGTAGGCAATTG1830.16288964449112564No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTCATA1800.1602193224502875No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCTCTGGTCGAAATTA1790.15932921510334147No Hit
CTTACCTGAGGAGACGGTGACCCTGGTCCCTTGGCCCCAGTGGCTAAGTT1770.15754900040944939No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTT1760.15665889306250336No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTCC1760.15665889306250336No Hit
CTTACCTGAGGAGACGGTGACCGTGGCCCCTTGGCCCCAGACGTCCATAC1750.1557687857155573No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGACCCCAGAAGTCAATTA1750.1557687857155573No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTCG1750.1557687857155573No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGTCATTGT1730.15398857102166522No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA1710.15220835632777313No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCACAA1710.15220835632777313No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC1690.15042814163388105No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGC1680.149538034286935No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA1680.149538034286935No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCTATAAGAG1670.14864792693998896No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGATACCAGC1670.14864792693998896No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGTAATCAAGGA1660.14775781959304293No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTTG1650.14686771224609688No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC1620.14419739020525876No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTGAAAGG1600.14241717551136668No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAAT1600.14241717551136668No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTCTGCCCCAGACGTCCATGT1580.14063696081747457No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGA1580.14063696081747457No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCTCAGTGTTGACGAG1580.14063696081747457No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGGAGTCAAGTG1560.1388567461235825No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTCGTGCAGC1560.1388567461235825No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCACAT1560.1388567461235825No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGAAATGAAAAC1560.1388567461235825No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTGGC1550.13796663877663648No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTTTCACTTC1550.13796663877663648No Hit
CTTACCTGAGGAGACGGTGACCATCGTCCCTTGGCCCCAAATATCAAAAG1540.13707653142969042No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCATGGCCCCAGACATCAAAAG1520.13529631673579834No Hit
CTTACCTGAGGAGACGGTGACCGGGATTCCCTGGCCCCAGTAGTCAAAGT1510.13440620938885228No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCTAACC1480.13173588734801417No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACGATATATAAC1480.13173588734801417No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCACAGATCAAAAG1480.13173588734801417No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT1460.12995567265412208No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGATGTGAT1440.12817545796023No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCATGA1440.12817545796023No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCAAAGT1430.12728535061328397No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCACTAGTAG1430.12728535061328397No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCCCGTAACCA1420.12639524326633791No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATATT1420.12639524326633791No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACGGCACAT1400.12461502857244583No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCGGGGGTCGAACC1390.1237249212254998No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGG1390.1237249212254998No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTCCCGTACC1390.1237249212254998No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTTGAGC1390.1237249212254998No Hit
CTTACCTGAGGAGACGGTGACCACTGTCCCTTGGCCCCAGATATCAAAAG1370.12194470653160772No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAATCAAATG1360.12105459918466167No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAGAG1350.12016449183771562No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGATTGTAGC1350.12016449183771562No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATGGA1350.12016449183771562No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGC1340.11927438449076959No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAATGC1340.11927438449076959No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACGCTAC1320.1174941697968775No Hit
CTTACCTGAGGAGACGGTGACCAGGCTTCCCTGGCCCCAGTAGTCAAAGC1320.1174941697968775No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCCCGTAGTTC1300.11571395510298543No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCAGGCCCCAGAAGTCAAGAT1280.11393374040909332No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTACCTGGCCCCAGAAGTCCGCCT1280.11393374040909332No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTTCTTCAAAACAGGAGAGAGGCT1280.11393374040909332No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGATC1270.11304363306214729No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCCCAGCGCTG1260.11215352571520125No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGG1250.11126341836825522No Hit
CTTACCTGAGGAGACGGTGACCAGACTTCCCTGGCCCCAGTAGTCAAATG1250.11126341836825522No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGATAAGCCGT1240.11037331102130916No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGC1240.11037331102130916No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGCAGTCCGGCA1220.10859309632741708No Hit
CTTACCTGAGGAGACGGTGACCTGGGATCCCTGGCCCCATTAGTCAAGGC1210.10770298898047104No Hit
CTTACCTGAGGAGACGGTGACCGAGATCCCTTGGCCCCAGACGTCCATCC1200.10681288163352501No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAGA1200.10681288163352501No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGTGGTCTAGTG1190.10592277428657895No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGGGTGACCACT1180.10503266693963292No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGAT1180.10503266693963292No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGGGGTCGAACG1160.10325245224574084No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAAAT1160.10325245224574084No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCTAATAACCA1160.10325245224574084No Hit
CTTACCTGAGGAGACGGTGACCACGGTTCCCTGGCCCCAGTACTCAGTCC1150.1023623448987948No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCTCGG1150.1023623448987948No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCCGTCAAAGG1150.1023623448987948No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCTT1140.10147223755184877No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGAGT1130.10058213020490271No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACACAAC1130.10058213020490271No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCGGGCCCCAGTATTTGTAAC1130.10058213020490271No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC1130.10058213020490271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)