FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414430
Sequences flagged as poor quality0
Sequence length47-300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC7720418.628960258668535No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT361998.734647588253747No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC252216.085708080978693No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG241095.817387737374225No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT135243.2632772723982337No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG68301.6480467147648576No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC64121.5471852906401564No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT45711.102960693000024No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC41220.994619115411529No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT34910.8423617981323746No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC33720.813647660642328No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA33470.807615278816688No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT21840.5269888762879136No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT21710.5238520377385807No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT20280.4893468136959197No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC18140.43770962526844104No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA17760.4285404048934681No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA17680.4266100427092633No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCAAAT17210.41526916487706006No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG17050.4114084405086505No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAGT14670.3539801655285573No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGA13820.3334700673213812No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCGTGGCCCCAGTAGTCAGAAA12540.3025842723741042No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC12470.300895205462925No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTC12280.29631059527543857No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG12020.2900369181767729No Hit
CTTACCTGAGGAGACGGTGACCAGGGATCCCTGGCCCCAGTAGTCAAACT11920.2876239654465169No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG10980.26494220978211036No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCGAAAC10970.2647009145090848No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGT10430.2516709697657023No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGGA10350.24974060758149746No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTT10330.24925801703544626No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC10220.24660376903216466No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC10090.24346693048283183No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGGACGGG10030.24201915884467826No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAT9990.24105397775257584No Hit
CTTACCTGAGGAGACGGTGACCAGAATTCCCTGGCCCCAGTAGTCAACCC9980.24081268247955023No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATCCCACGG9790.23622807229206377No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGTCCCCCGATT9640.23260864319667976No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG9540.23019569046642377No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACTCCCCCAA9330.22512848973288616No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCCCCCC9270.22368071809473253No Hit
CTTACCTGAGGAGACGGTGACCACTGTCCCTTGGCCCCAGATATCAAAAG9250.22319812754868132No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG9180.22150906063750211No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAAA9180.22150906063750211No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGATT8990.21692445045001568No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC8930.21547667881186208No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGACCCGGGCGA8830.21306372608160604No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCC8820.21282243080858043No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGGCGTCCAGAC8820.21282243080858043No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTGTTC8780.21185724971647807No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCGCCT8640.20847911589411966No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCTGGGC8590.20727263952899164No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCTCCTAAAAATAAC8540.2060661631638636No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCCAGCG8490.20485968679873562No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATGGT8460.2041358009796588No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCAGAGTAGTAGCTC8420.2031706198875564No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGAAAG8310.2005163718842748No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACGGT8300.2002750766112492No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAAC8270.19955119079217237No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT8260.19930989551914677No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT8260.19930989551914677No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATGTG8180.19737953333494196No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGTCCCCAGTAGTCAAAGT7890.1903819704171995No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCTCGTGT7870.18989937987114833No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGCCG7850.18941678932509712No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGC7820.1886929035060203No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCTTTTTGGGTCCC7790.18796901768694352No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGGAG7600.18338440749945709No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCTG7590.18314311222643148No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGTT7580.18290181695340588No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTAGCTGG7570.18266052168038027No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG7520.18145404531525228No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG7500.18097145476920107No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCATGTAGTGATAT7490.18073015949617546No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCCCGAGGGG7460.18000627367709868No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGT7440.17952368313104747No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTCGGGGCCCCGGTGCTATAC7380.17807591149289387No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCATGGCCCCAGAAGTCCCAAT7370.17783461621986826No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAGTCATCCT7270.17542166348961222No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGATAATAACCA7270.17542166348961222No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCACAC7250.17493907294356104No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCAC7240.17469777767053543No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGATCCGGAT7190.17349130130540744No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTGATGTC7120.17180223439422823No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGT7070.1705957580291002No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCCC6990.1686653958448954No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAC6970.1681828052988442No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGGGTTCG6950.16770021475279298No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA6850.165287262022537No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCAAACCAGTGCCAGG6840.16504596674951139No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCGAT6770.16335689983833218No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCATAAT6710.16190912820017855No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGGGCCGCT6630.15997876601597374No Hit
CTTACCTGAGGAGACGGTGACCAGGCTTCCCTGGCCCCAGTAGTCAAGCC6610.15949617546992254No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAACAT6530.15756581328571773No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGAATCCGT6230.1503269550949497No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCTACCAGCCA6180.14912047872982168No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGGGGG6170.1488791834567961No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCGGCAG6150.1483965929107449No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCCCCGT6140.14815529763771929No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAT6140.14815529763771929No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGCTCATCG6120.14767270709166808No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGAGTTGGT6080.14670752599956566No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCTT6020.14525975436141206No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAAGGCTCTG6000.14477716381536085No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGAGGGGAT5990.14453586854233524No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGTCAAAGG5900.14236421108510486No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTCCAAA5870.14164032526602804No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCGGGCCACT5870.14164032526602804No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAAT5830.14067514417392565No Hit
CTTACCTGAGGAGACGGTGACCAGGCTTCCCTGGCCCCAGTAATTAGGGC5780.13946866780879763No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTGG5780.13946866780879763No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC5600.1351253528943368No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCGAGACCG5560.1341601718022344No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCCGTCG5520.133194990710132No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAAGGGACTGCTG5510.1329536954371064No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTGTCC5510.1329536954371064No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCGGGGGTAGTAATAAC5370.12957556161474795No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGACAAACA5360.12933426634172235No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGGTAG5350.12909297106869677No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCGG5330.12861038052264556No Hit
CTTACCTGAGGAGACGGTGACCAGAGTTCCCTGGCCCCAGTAGTCAAAGT5300.12788649470356875No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCACAAAAGACCCCT5230.12619742779238954No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTCCCCATAAAAATCAACCA5220.12595613251936394No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGTCCATTC5120.12354317978910793No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAGTG5060.12209540815095432No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCCCGGCTGCT5010.12088893178582633No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGGCACCGG5000.12064763651280072No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGT4960.1196824554206983No Hit
CTTACCTGAGGAGACGGTGACCAGGATTCCCAGGCCCCAGTACGGCACGA4950.1194411601476727No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATAAGTAGC4910.11847597905557031No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCACTACACAAACT4860.1172695026904423No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATGAC4850.1170282074174167No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGT4820.11630432159833988No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTAAAA4800.1158217310522887No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTTTTCGGGG4790.1155804357792631No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTACAG4770.11509784523321188No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACAAA4740.11437395941413508No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC4740.11437395941413508No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAAGT4700.11340877832203267No Hit
CTTACCTGAGGAGACGGTGACCAGCATTCCCTGGCCCCATTACTCCGCTC4640.11196100668387907No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTATCCAA4640.11196100668387907No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCAGTCAAAGA4630.11171971141085346No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCATAGT4620.11147841613782786No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTG4620.11147841613782786No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGG4570.11027193977269985No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCCAACC4540.10954805395362303No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATAATAACC4510.10882416813454625No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCTGGT4470.10785898704244383No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGGGGAAGG4450.10737639649639263No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAGC4420.10665251067731582No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAATCCGCCT4400.10616992013126464No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTGCATAC4350.10496344376613662No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAAGGGTCTT4340.10472214849311101No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAT4320.10423955794705983No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTTGTTTT4270.1030330815819318No Hit
CTTACCTGAGGAGACGGTGACCGTGGCCCCTTGGCCCCAGACGACGAAAG4270.1030330815819318No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTGGGA4270.1030330815819318No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTACATCC4190.10110271939772701No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATAATCCAC4190.10110271939772701No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTCCAA4180.10086142412470141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)