FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001132542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760720
Sequences flagged as poor quality0
Sequence length35-300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC16961322.29637711641603No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT565717.436507519192344No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC498816.557077505521085No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG410435.3952834157114316No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT189722.4939530970659374No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG101031.328083920496372No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT79471.0446682090650963No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC79321.0426963928909454No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC74070.9736828267956672No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA71000.9333263224313808No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT49990.6571406036386581No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG44210.5811599537280471No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC43000.5652539699232306No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT39790.5230571037964034No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC38240.5026816699968452No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG35680.46902934062467133No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT34900.4587758965190872No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG27470.36110526869281734No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG26540.34888000841308237No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA23390.3074718687559154No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGT23180.30471132611210433No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT21730.2856504364286465No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC21320.28026080555263433No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAA20780.27316226732569143No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC20570.27040172468188034No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCATGGCCCCAGACGTCCAAAC20420.2684299085077295No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCGCCT20370.26777263644967925No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAC20260.26632663792196865No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAAATATCAAAAC19760.25975391734146597No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG19720.2592280996950258No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCGGCA19440.2555473761699443No Hit
CTTACCTGAGGAGACGGTGACCGCGGTCCCTTGGCCCCAGACGTCCATGT18890.2483173835313913No Hit
CTTACCTGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGGGGTCGAACC18700.24581974971080028No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGC18230.2396413923651278No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTACCAGCCA17940.23582921442843624No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATCTATAGCC17850.23464612472394575No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG17010.22360395414870124No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGAC16920.22242086444421075No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCATGGCCCCAGACGTCCATAC16820.2211063203281102No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTA16600.21821432327268903No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTTAAGCAGC16490.21676832474497842No Hit
CTTACCTGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGTAGTCAATTT16440.21611105268692815No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCC16360.21505941739404774No Hit
CTTACCTGAGGAGACGGTGACCGCGGTCCCTTGGCCCCAGACGTCCATAC16320.21453359974760752No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTGGGCCCCAGACGTCCAGAC16240.2134819644547271No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTTGCCCCAGACGTCCATGT16200.21295614680828687No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGGAGTCAATGT15860.20848669681354506No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATTCAAAGA15390.20230833946787252No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCTTGGCCCCAGTAGTCAATGT14900.1958670732989799No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCC14870.19547271006414976No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC14860.19534125565253968No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC14800.19455252918287938No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCA14750.1938952571248291No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAAGCTTCTC14660.19271216742033861No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCGGG14530.19100326006940793No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGTTG14510.19074035124618782No Hit
CTTACCTGAGGAGACGGTGACCAGCGTTCCCTGGCCCCACTGGTCAAGCA14470.1902145335997476No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA14470.1902145335997476No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGT14450.1899516247765275No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAATTCATAA14450.1899516247765275No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGAGA14310.18811126301398676No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCGG14140.18587653801661583No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTC14010.18416763066568514No Hit
CTTACCTGAGGAGACGGTGACCACGGTTCCCTGGCCCCAGTAGTCAAAGT13930.1831159953728047No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGACTAGTA13800.18140708802187402No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCCTGTGGGTT13670.1796981806709433No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTACACTG13660.17956672625933326No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCGCAGTAGTCAAAAC13540.17798927332001263No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTG13420.17641182038069197No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCGGTAGTCAAAGT13380.17588600273425176No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGA13360.17562309391103165No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA13270.17444000420654118No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCCC13180.1732569145020507No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCGCC13060.17167946156273003No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGTAGTCAAAGA13000.17089073509306973No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT12920.1698390998001893No Hit
CTTACCTGAGGAGACGGTGACCATTGCCCCTTGGCCCCAGATATCAAAAG12850.16891891891891891No Hit
CTTACCTGAGGAGACGGTGACCTGGATTCCCTGGCCCCAGTAGACCCCCA12820.16852455568408875No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAACC12780.16799873803764856No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAGTCAGTGC12670.16655273950993796No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAAT12540.16484383215900728No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCTAGAA12490.164186560100957No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAAC12480.16405510568934695No Hit
CTTACCTGAGGAGACGGTGACCAAGGATCCCTGGCCCCAGCAGTCAGATT12430.16339783363129667No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAGC12060.15853402040172468No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCCATAA12060.15853402040172468No Hit
CTTACCTGAGGAGACGGTGACCAGGACTCCCTGGCCCCAGTAGTCAAAGT11980.15748238510884424No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACGGCGACTCCC11580.1522242086444421No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGG11530.15156693658639184No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGCCC11370.14946366600063096No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTCG11200.14722894100326006No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAC11160.14670312335681987No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTAGG11150.1465716689452098No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAAT11130.14630876012198968No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTTCGGGGGA10940.1438111263013987No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGA10890.1431538542433484No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTAAC10880.14302239983173837No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACTGT10850.1426280365969082No Hit
CTTACCTGAGGAGACGGTGACCAGAGTTCCCTGGCCCCAGTAGCCCCCTG10840.14249658218529812No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAG10830.14236512777368807No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCAAT10800.14197076453885793No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGTTGGAAGT10700.14065622042275738No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACATCTA10600.13934167630665684No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCT10500.13802713219055632No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGCCCTAGTA10470.13763276895572615No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAGCC10420.13697549689767588No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCTATAC10220.13434640866547481No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAAAA10180.1338205910190346No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGATGTAAT10160.1335576821958145No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGCAC10140.13329477337259438No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTC10130.13316331896098432No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACAG10120.13303186454937427No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACTCG10060.13224313807971394No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGCAGTCATCCC10040.13198022925649386No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAGTCATTTA10040.13198022925649386No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACTGGTCGAATA10030.1318487748448838No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTGTCGAACC9970.13106004837522348No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCTAATA9970.13106004837522348No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGTG9900.1301398674939531No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGTAGTCCAAGT9850.12948259543590282No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTTATGT9810.12895677778946263No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCAACTATGCC9680.12724787043853192No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC9660.1269849616153118No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAAC9620.1264591439688716No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATCCGAGCG9560.1256704174992113No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGCC9410.12369860132506047No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCT9340.12277842044379009No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAAAC9320.12251551162056999No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGATAG9270.12185823956251973No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGAGGTAGC9220.12120096750446945No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG9200.12093805868124935No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAGT9190.1208066042696393No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAGGG9180.12067514985802924No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC9140.12014933221158902No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAA9090.11949206015353876No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGCACAAT9080.11936060574192871No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAT8990.11817751603743822No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACTGACCCCGGA8880.11673151750972763No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGAC8840.11620569986328741No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAGTCAAAGT8680.11410242927752655No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCGGGA8670.11397097486591651No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAACAATCGT8510.11186770428015563No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGG8450.11107897781049532No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAACGAATGGC8400.11042170575244505No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCTACT8370.1100273425176149No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGGCCAAGT8280.1088442528131244No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTAAT8210.10792407193185402No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCAATAC8200.107792617520244No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC8200.107792617520244No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTCCCCG8140.10700389105058367No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC8100.10647807340414343No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAGA8040.10568934693448312No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGAGATCAAAAG8020.10542643811126302No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGACC7880.10358607634872227No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATTC7780.10227153223262174No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGGG7750.10187716899779156No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGTCAAACT7630.10029971605847092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)