FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001132545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800438
Sequences flagged as poor quality0
Sequence length35-300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC15291819.10429040100545No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC591047.383957283387345No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT564097.047266621524716No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG561577.01578385833756No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT195662.44441168460268No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC142071.7749032404758396No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT93311.1657367591243795No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC84681.0579207883683683No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG83891.0480511919723952No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC82241.0274374779808055No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA74000.9244938396228065No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT54160.6766295453239352No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG53850.6727566657255153No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG44690.5583193201722056No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA43950.5490743817759777No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC41350.5165921657892304No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG34990.4371356682216486No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT34970.4368858050217506No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA32420.40502824703474843No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC30900.38603864384249625No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG25240.31532735827134645No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC25230.3152024266713974No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGG23470.2932144650803685No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC21160.26435526549214305No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC20050.2504878578978009No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC19620.24511579909999273No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT19270.24074319310177678No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAATC19000.23737003990315303No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAACC18220.22762537510712882No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG17740.22162865830957548No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAC17690.22100400030983036No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTC17550.21925495791054397No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT17140.21413276231263384No Hit
CTTACCTGAGGAGACGGTGACCACTGTCCCTTGGCCCCAGATATCAAAAG16690.20851084031492756No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT16690.20851084031492756No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC16440.20538755031620187No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAAGT16250.20301384991717034No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA15600.19489329592048354No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA15380.19214480072160492No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT14230.17777766672746673No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG14020.17515410312853713No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGT12900.161161763934246No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC12590.15728888433582613No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG12280.15341600473740627No Hit
CTTACCTGAGGAGACGGTGACCAATGTTCCCTGGCCCCAGTAGTCCAGAA11970.1495431251389864No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAGTCAAAGT11750.14679462994010778No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAGG11600.14492065594087236No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAC11520.14392120314128015No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGC11410.14254695554184085No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC10850.13555078594469527No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG10830.13530092274479721No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGA10590.13230256434602056No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTA10380.12967900074709096No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATACC10250.1280548899477536No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATTGTCAAAGG10040.12543132634882403No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGGAGTCAAAGG9760.12193324155025125No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT9560.11943460955127069No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCACTGGCCCCAGAAGTCCAATC9550.11930967795132166No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG9400.11743570395208623No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAGGT9320.11643625115249402No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCGTGT9300.11618638795259596No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAAAT9290.11606145635264693No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCTTGC9260.11568666155279984No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC9230.11531186675295275No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTAAT9170.11456227715325859No Hit
CTTACCTGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGTCGAGACTAA9140.1141874823534115No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT9030.1128132347539722No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACA8990.11231350835417608No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCCACAC8980.11218857675422705No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG8910.11131405555458386No Hit
CTTACCTGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGCTAGCCTGAG8810.11006473955509359No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCGTGGCCCCACATATCAAAAG8750.10931514995539943No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTG8710.10881542355560331No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCA8670.10831569715580719No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGTG8440.10544227035697955No Hit
CTTACCTGAGGAGACGGTGACCAGGGACCAGGGTTCCCTGGTCCCAGCAG8370.10456774915733637No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCCCATAACG8280.10344336475779511No Hit
CTTACCTGAGGAGACGGTGACCAGAGTTCCCTGGCCCCAGCCTGAGTAGC8200.10244391195820288No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAG8130.1015693907585597No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCACCAGTCAAAGT8110.10131952755866164No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGACCCCGGTTA8100.10119459595871261No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCATAGGGAATCTC8090.10106966435876358No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGCCATAA8050.10056993795896747No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCATC8020.10019514315912038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)