FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336191
Sequences flagged as poor quality0
Sequence length47-300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT3573910.63056417334194No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC3426710.19271783004304No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC327979.755466386667102No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG308279.16948996255105No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT250887.462424633616011No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC77662.309996400855466No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC49901.4842753077863475No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT39721.1814712469994735No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC32930.9795027231543972No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG23420.6966278097866987No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG21730.6463587662965398No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA21260.6323786181069689No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG21020.6252398190314434No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA20730.6166137701485168No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC15030.4470672921047857No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC14960.44498514237442405No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT14070.4185120958026836No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG13520.4021523479212709No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG13360.3973931485375872No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTT13070.3887670996546606No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTA12650.3762742012724909No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACA12160.36169915315995965No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGG11460.34087765585634355No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC10310.30667091028611715No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTT9020.2682998652551674No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG8980.26711006540924653No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAG8860.2635406658714838No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATGGG7850.23349821976198054No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT7620.22665687064793524No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC7580.22546707080201434No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC7240.21535377211168652No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT7030.20910732292060166No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGG6700.1992914741917541No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGATAACGATAA6590.19601952461547156No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAACCATAGC6390.19007052538586697No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAAAATAGGG6340.18858327557846583No Hit
CTTACCTGAGGAGACGGTGACCGTAGTCCCTTGGCCCCAGACGTCCATAA6280.18679857580958442No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCCCAG6250.18590622592514375No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGG6110.18174192646442053No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAC6040.17965977673405892No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCACCCAGTTATAA6020.17906487681109845No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAA5830.1734133275429741No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC5770.1716286277740927No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGCCGTCC5730.17043882792817178No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT5650.16805922823632996No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG5470.1627051289296858No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGGTATTAAAAG5370.1597306293148835No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTT5360.1594331793534033No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAAGG5350.15913572939192303No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGAAAT5260.15645867973860098No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC5220.15526887989268007No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCATGGCTCAGCCG5210.1549714299311998No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCACAC5190.15437653000823937No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT5110.15199693031639752No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCTTGGCCCCAGTAGTCAAAGG5080.15110458043195685No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCGAATGACAGACGA5040.1499147805860359No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCTTGG5020.14931988066307547No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAAG4960.14753518089419407No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAATA4830.14366833139495108No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGAG4730.14069383178014877No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT4620.13742188220386625No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCTGGGAAAT4600.13682698228090578No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATGTCTGGGA4570.1359346323964651No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAACCACTACTAT4570.1359346323964651No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC4560.13563718243498488No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAATAA4500.1338524826661035No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCGAACCATAG4490.13355503270462327No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGTAA4380.13028308312834075No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG4380.13028308312834075No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGGGAACCA4330.12879583332093958No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTCCAG4250.12641623362909774No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTCCTT4240.12611878366761753No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCGGCAGT4230.1258213337061373No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTTC4220.12552388374465706No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAACC4220.12552388374465706No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCGCCGAGAC4220.12552388374465706No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAT4210.12522643378317683No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGG4200.1249289838216966No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAAGGCGACTAAA4150.12344173401429544No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACGCT4150.12344173401429544No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCTC4140.12314428405281522No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCATACA4110.12225193416837453No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAAAT4090.12165703424541408No Hit
CTTACCTGAGGAGACGGTGACCCGGCAGCAGCACATGGCACCCGCGCAGA4060.12076468436097336No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACGTTAAAACGG4050.12046723439949314No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGT3980.11838508466913153No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAACTAG3960.11779018474617108No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG3920.11660038490025017No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCAGA3910.11630293493876992No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCA3900.1160054849772897No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT3870.11511313509284901No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGGAAAGGG3840.11422078520840831No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACAAAGTAC3820.11362588528544784No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCACG3820.11362588528544784No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTGCTCCA3780.11243608543952693No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAAAT3730.11094883563212579No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCAAAT3730.11094883563212579No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGT3700.11005648574768509No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGAC3670.1091641358632444No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTCC3660.10886668590176418No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACAGATAGCAGC3640.10827178597880371No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTC3620.10767688605584326No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTC3620.10767688605584326No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGGGGTAGA3600.10708198613288279No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCCCAAGCTAT3570.1061896362484421No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACAAA3490.10381003655660027No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCTATCCCCA3490.10381003655660027No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTACGAAA3490.10381003655660027No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCATAAGATAACTC3480.10351258659512003No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCTACGATTA3470.1032151366336398No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT3460.10291768667215957No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGTG3450.10262023671067935No Hit
CTTACCTGAGGAGACGGTGACCAAAATTCCCTGGCCCCAGTAGCCAAAAA3410.10143043686475842No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTCCC3390.10083553694179795No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG3380.10053808698031773No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGGAT3370.10024063701883751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)