FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310911
Sequences flagged as poor quality0
Sequence length55-300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT5397917.36156006059612No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC3344810.758062596691657No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG283429.1157919790551No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC282709.092634226514985No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT172715.554965890560322No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT54181.7426208786437276No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC34801.1192913727722724No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG30530.9819530347913068No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG26120.8401118004830964No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG23320.750053873938201No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG23090.7426562585434416No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA20710.6661070209802805No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC20430.657101228325791No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT18980.6104640877936129No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGTA18540.5963121279079865No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC18360.5905226897729575No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTT17530.5638269472614349No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGC17130.5509615291835928No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCCC16850.5419557365291032No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTACCAGC16450.529090318451261No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATACG16280.5236225157681781No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCTT16020.5152599940175806No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGG15540.49982149232416995No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCG15190.488564251506058No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGATCA15080.48502626153465145No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCCG14590.46926612438929466No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTAGGA14420.46379832170621177No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGAAA13870.44610837184917873No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCATCAT13710.4409622046180418No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGTCCAGCCA13630.43838912100247335No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATCG13510.4345294955791207No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATAGGTCGG13110.4216640775012785No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCTAGATC12870.4139448266545732No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACGTAGCAATCA12840.412979920298735No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAACCCGATTCGA12660.407190482163706No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGAGACGAG12650.40686884671176No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACTAA12450.40043613767283887No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAAGA12220.3930385222780796No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCTCGT11940.38403272962359003No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTATAGTA11860.3814596460080216No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAG11730.3772783851327229No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT11670.3753485724210465No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGAGGCCTA11450.3682725924782333No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCAT11430.3676293215743412No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCA11060.3557288098523372No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGG10770.34640138174590157No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT10680.34350666267838703No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTA10650.3425417563225489No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAACACACGC10090.3245301710135698No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCAAGAC10040.3229219937538395No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAG9720.3126296592915658No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC9620.3094133047721052No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCCC9590.30844839841626703No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGTCC9590.30844839841626703No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGGG9490.30523204389680647No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCACTATTCAAAAC9440.3036238666370762No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACAAATA9430.30330223118513017No Hit
CTTACCTGAGGAGACGGTGACCGTGGCCCCTCTGCCCCAGACGTCCATGT9350.3007291475695617No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG9330.3000858766656696No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGG9170.2949397094345327No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGCCCCGGC9130.2936531676267485No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGAATAATA8890.28593391678004315No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG8880.28561228132809713No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGAGAAACCACTCCAAGG8740.28110938500085236No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGCTAGTA8520.2740334050580391No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACCTATC8410.2704954150866325No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCAAAAGATCTAACG7950.25570018429711394No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCGT7830.2518405588737613No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT7410.23833186989202698No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACATA7340.2360804217284046No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAGG7260.23350733811283614No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGGGGA7240.23286406720894404No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA6640.21356594009218072No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA6520.20970631466882805No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCG6210.19973561565850032No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAAG6090.1958759902351477No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCATTG5820.1871918330326042No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTTGAACC5740.18461874941703574No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTTAG5720.18397547851314364No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGAAACCAC5660.1820456658014673No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCAA4830.1553499232899447No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG4010.1289758162303682No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC3760.1209349299317168No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC3440.11064259546944301No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC3160.10163680281495346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)