FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451835
Sequences flagged as poor quality0
Sequence length45-300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC6348414.05026171058019No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT5671812.552812420463223No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC4593810.166985735943431No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT4545010.058981707924353No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG339297.509157103810018No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC68801.5226797392853586No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT61621.363772173470404No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG45231.001029136742395No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG42060.9308707824759038No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT41740.9237885511303905No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA38480.8516383192979738No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC38140.8441134484933659No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG37900.8388017749842309No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC36490.807595693118063No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT34490.7633317472086049No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA26710.591144997620813No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTG26050.5765378954706918No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC22050.4880100036517755No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGCCGCACG19650.4348932685604258No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCAG19600.4337866699126894No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAGGT19110.4229420031648721No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATGTT18950.41940088749211546No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAG18880.4178516493852844No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCCCATAC18870.41763032965573715No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT18450.40833490101475095No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT18450.40833490101475095No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTAT17910.39638363561919726No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGG17530.38797348589640024No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAT16170.35787400267796876No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGA15960.3532262883574756No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCCGTT15770.3490212134960771No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCATCCT15630.34592273728241507No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCAAGTGG15130.3348567508050505No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAATACCAAT15120.3346354310755032No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAG15120.3346354310755032No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG14390.318479090818551No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTGGCAAC14200.3142740159571525No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAAAC13960.3089623424480175No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTCCCC13830.3060851859639027No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTA13760.3045359478570717No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACAG13470.2981176757002003No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT12880.28505981165691013No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGA12870.2848384919273629No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGTA12770.28262529463188996No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACAT12100.26779687275222147No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGT11780.2607146414067082No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC11750.2600506822180663No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAAACCCATTCGG11170.2472141379043235No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATATC10760.23814002899288458No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGTAA10760.23814002899288458No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCTCAT10740.23769738953379No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTCAAAG10670.23614815142695894No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGTAT10550.23349231467239148No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTC10490.23216439629510774No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCTGTT10290.22773800170416192No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGC10190.225524804408689No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAAA10070.22286896765412154No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC10000.22131972954729048No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGACC9610.21268826009494618No Hit
CTTACCTGAGGAGACGGTGACCGCGGTCCCTTTGCCCCAGACATCCATGT9490.2100324233403787No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGA9450.2091471444221895No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTAAGT9420.20848318523354767No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCA8550.18922836876293336No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTAG8490.18790045038564962No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCTCTAC8340.18458065444244026No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT8270.18303141633560924No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGC8200.18148217822877818No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTC8090.179047661203758No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGCAAGTTAC8090.179047661203758No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGT7880.1743999468832649No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAGAG7790.17240806931733932No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGC7300.16156340256952206No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGC7280.1611207631104275No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG7140.15802228689676542No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCGGGGCACTGTG6960.1540385317649142No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGGATAAC6870.15204665419898858No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC6710.1485055385262319No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCTAGC6690.14806289906713735No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTA6600.14607102150121173No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG6520.1443004636648334No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC6510.1440791439352861No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTCCAGG6500.14385782420573884No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG6330.1400953888034349No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGCC5990.13257051799882702No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC5970.13212787853973243No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAA5960.13190655881018515No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCTATCCGCAG5940.13146391935109056No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAAAGATCGAAGT5690.1259309261124083No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGGGGTCGAATC5640.12482432746467184No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGTTCCCGGGGT5610.12416036827602997No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTCAAT5560.12305376962829351No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCCTTCCATA5070.11220910288047628No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG5050.1117664634213817No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACGTAAG5020.11110250423273982No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGG4580.10136443613265904No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG4570.10114311640311176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)