FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001132550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714440
Sequences flagged as poor quality0
Sequence length35-300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC13643919.09733497564526No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC493206.903308885280779No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT485866.800571076647444No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG351474.919517384245003No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT198142.7733609540339286No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG142561.995408991657802No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC91511.2808633335199597No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC88321.2362129779967528No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC82211.1506914506466603No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT69810.9771289401489278No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC62820.8792900733441575No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG61240.8571748502323497No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG61130.8556351828005151No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA46630.6526790213313924No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT44000.6158669727338895No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC42000.5878730194278036No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG41100.5752757404400649No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG40090.5611387940204915No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC23730.33214825597670905No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT21820.305414030569397No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT21770.30471418173674486No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA21670.30331448407144057No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG19770.27672022843065897No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTC19560.2737808633335199No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC19210.26888192150495493No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC18890.2644028889759812No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC17710.24788645652539051No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC15920.22283186831644364No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT13950.19525782430994906No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC13890.19441800571076648No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC13540.18951906388220147No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA13420.1878394266838363No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG13400.18755948715077544No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGCG13300.18615978948547113No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAAAAG13120.1836403336879234No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT13120.1836403336879234No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG13050.1826605453222104No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC12270.17174290353283692No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAAG12160.1702032361010022No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAAGG11580.16208498964223728No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGG11550.161665080342646No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG11380.1592855943116287No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG11260.15760595711326353No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATACG11080.1550865013157158No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTA10870.15214713621857678No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTT10700.14976765018755947No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC10660.14920777112143777No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG10520.14724819439001177No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT10270.14374895022675102No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCT10230.14318907116062932No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT10230.14318907116062932No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT9980.13968982699736857No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT9710.135910643301047No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC9430.13199148983819495No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGC9300.13017188287329937No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC9220.12905212474105593No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTTG9200.12877218520799508No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAAA9180.1284922456749342No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAC9130.12779239684228208No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAAGT8870.1241531829124909No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAAGG8560.11981412015004758No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC8550.11967415038351716No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAATCTTATG8500.11897430155086502No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG8460.1184144224847433No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTACCTGGCCCCAGGGGTCCAACC8450.11827445271821285No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAAACGTCCATAA8450.11827445271821285No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGT8270.11575499692066513No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTGTGCCCCAGACGTCCATGT8240.11533508762107386No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGG8220.11505514808801298No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCAGGC8180.11449526902189128No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGGGG8150.11407535972229998No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAATAC8020.1122557527574044No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAT7980.11169587369128268No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA7850.1098762667263871No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAACC7770.10875650859414367No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAATCGTAGG7760.10861653882761324No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACATTCGACT7740.10833659929455237No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAGAAA7700.10777672022843066No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAATAAC7460.10441744583170036No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATGTCAAAGC7420.10385756676557863No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAATC7370.10315771793292648No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGATGCAAA7330.10259783886680476No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAATT7260.10161805050109177No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA7230.10119814120150047No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGTAGTCAAAGT7190.10063826213537876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)