FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950438
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC498835.248422306347178No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG110321.1607280011952386No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGACCAG88450.9306235651352324No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA79170.8329843714161259No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACGAGCTGCTATT57460.6045633697305873No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAAAGCA49860.5246002369433882No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGCGATAGCAGCTGGTACTAC14060.14793179565631845No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGACATAGCAGCTGGTACTAC10850.11415789351856723No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGTTACTAC10630.11184317125367463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)