FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318591
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCATAGCAGCTGGTACTA39911.252703309258579No Hit
ATTGTCCCTTGGCCCCAGATATCAAAACCAACCCCCCATGAGTACGACCC39831.2501922527629468No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTATTGATCACC39371.235753677913061No Hit
CTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAAGTGGGAGTCGTCCGG38361.204051589655703No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTCCCCTGGATAACCAGT38021.1933795995492653No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTCTGAGGGACGAAA37971.1918101892394952No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAACCGGAGTAGTAGGACGTACCACC36791.1547721059289184No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGCACAAAATAAT36101.1331142436540895No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTTTTCCCCCGCTGA35561.1161646123085713No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCATTCCCCGAACCC35181.1042370939543178No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAGAAGTAGTAGGACCTGGC35001.0985872168391448No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGAGTCCAGCCACTGCTATACACTCT34701.0891707549805236No Hit
TCCCCAGTCAGGGTGAGTAGTGGTGGGGAGCCGGGCAGGGGCCCAGCCCT34551.084462524051213No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTTCCAATA34151.0719072415730513No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCATTGGCCACTATCATAGTA34081.0697100671393729No Hit
GTAGTCCCTTTGCCCCAGACGTCCAAATGGGAATAGTCTGGTTTTGACGA33191.0417745636254634No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGGATAAC32971.0348691582624743No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCGTAGTTACT32141.008816947120289No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGTATGGGCAAAGCCC31820.9987727211377597No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGAACCTCCCCGAACCATAGTACC31800.9981449570138516No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATTCCGC31050.9746038023672986No Hit
GAGGTCCCTTTGCCCCAGACGTCCATGTAGTAGCCAAGGTTAAATCGTAG30500.9573402889598263No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTCAAGAACTCCCCG30180.9472960629772968No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTCCGGGGCTGGTACTAC30160.9466682988533889No Hit
AGGGTTCCCTGGCCCCAGTAGTCATACCTCCCCCGGCTGCTGCCGACACC29350.9212438518351116No Hit
GTGGTCCCTTTGCCCCAGACGCCCATGTACTGGTAGCGCCAGCACAACCC29010.9105718617286741No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAATCCTC29000.9102579796667201No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGGTAGTCATTATACCCCCCGTA28890.9068052769852255No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGTTATAGCAGCTGGTACT28710.9011553998700529No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAATAGCA28390.8911111738875235No Hit
AGGGTTCCCTGGCCCCAGTAGTCCGTTGTTCCCCATCCCCACCTCTGTTG28080.8813808299669483No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGAGCTGTATCCACCACCTCTTGC27830.8735337784180971No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGGTGCTGTC26960.8462260390280956No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGAA26710.8383789874792446No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCTCACTAGCCACTAGTATCCCA26700.8380651054172905No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGGG26690.8377512233553365No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAATAACAGTCACTACCACAATATGC26080.81860441757614No Hit
AGGGTTCCCTGGCCCCAGTAGTCGGGTAATAACCAGTCAACCGCAGCGTC25600.8035380786023459No Hit
GCGGTCCCTTTGCCCCAAACGTCCATGTAGTGAAGTGCTGCTATTCGATC25190.7906689140622302No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAAGTAGTAGTCCTCTCT25080.7872162113807358No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCCGA24980.7840773907611953No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCAGCTGCTGCT24330.7636750567341827No Hit
AGGGTTCCCTGGCCCCAGTAGTCATTGTAGCACCAGTTTTGTCCCGTTTT24330.7636750567341827No Hit
AGGGTTCCCTGGCCCCAGTGGAAACGAATAAATAACCACTCCAAAAATCG23850.7486087177603887No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTATGTACGGTGGAGTCCCCGAACC23600.7407616662115377No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAGATAGCAGCTGGTACTACTACAAT22420.7037235829009608No Hit
AGGGTTCCCTGGCCCCAGTAGACTGTACCAGTCACTGATCCCCGACAGTA22410.7034097008390068No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCTGCCGTCGGATAGCA22360.7018402905292366No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATGGCGCAGCTACCACCAACACTTG22220.69744594166188No Hit
AGGGTTCCCTGGCCCCAGTAGTAGTAGCTCCCACTACGTCGGGTGGTACA22090.6933654748564775No Hit
ATTGTCCCTTGGCCCCAGATATCCGGCAAGTTTTTCCAGTTCCCGGAGAA21440.6729631408294647No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGGGCAGCTGGTACTACTACAATATC21220.6660577354664758No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACCCCGTCGTAGTC21130.6632327969088895No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATCACCATCGCTGTACCAGCTGAAGT20810.6531885709263602No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGGGCCCCGGCTCCCGAGCTGCCC20570.6456554014394631No Hit
AGGGTTCCCTGGCCCCAGTTATCAAACACGTTTCCACTCCAAAGGTGCCC20550.645027637315555No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTTGTAGTAGTCAGAAGG19950.6261947135983126No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGTA19690.6180337799875075No Hit
AGGGTTCCCTGGCCCCAGTAGTCACTGATAGCAGCTGGTAGTAAGGACCG19570.6142671952440589No Hit
GTGGTCCCTCTGCCCCAGACGTCCATGTACGTCCCGGGGCCCCTCGCACA19550.6136394311201508No Hit
AGAGTTCCCTGGCCCCAGAAGACCGCATAGCAGGTGGTACTACTGCAAAA19040.5976314459604948No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAACCCCG18460.5794262863671604No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGGGGAAATCGTAGCCACTATA18170.5703237065704931No Hit
CCAACATGCGGCCTTTACCCTGGGGAGGTGGGGCGGGAACATTCCCTGGA18070.5671848859509528No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAATAACCTCCCTTCACTCT17610.5527463111010669No Hit
GTGGTCCCTTTGCCCCAGACGTCCTGCGGGAAATAACCACTCCAAAAATC17110.5370522080033648No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGGGCCCGGGGGAGCTGCTATA16880.5298329205784219No Hit
AGGGTTCCCTGGCCCCAGTTGGCAAATCCCGATAACAGAATTGCAGCTGC16810.5276357461447435No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGATAACCACTCCAAAAATCGTA16500.5179054022241683No Hit
AGGCCTCCCTGGCAAGTCGGCCAGGTGCTGCTTTACACATCGCCCCCTCG16390.5144526995426738No Hit
AGGGTTCCCTGGCCCCACTAGATAGCTGTATCCCCCCCCCAAACTCGCAC16150.5069195300557768No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCGGGAATAGCAGTCACCACC15700.49279483726784495No Hit
AGGGTTCCCTGGCCCCACCATTCACCAGCCTCACAATAATAGACGGCCGT15350.48180896509945353No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGATAATAACCACTCCAAAAATCGTA15170.47615908798428075No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAAGAGGTCTCCCTGACGTTATCTT14670.4604649848865787No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATATACCATAGCTGTATCCTAGCCC14500.45512898983336003No Hit
AGGGTTCCCTCCTTATAACTCTCGCACAGTAATACACAGCCGTGTCCTCG14330.44979299478014134No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGAGTAGTATTCGGG14330.44979299478014134No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGACCCCCTGGCCACTATATCC13730.4309600710628988No Hit
AGACTTCCCTGGCCCCAGTACCACAGCGAGCAGCTGGTACTAGTACAATC13560.4256240760096801No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAATCACTACTATCATAGGG13500.42374078363795586No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTCGTAGTCACCTAGTTCTCT12840.40302456754898913No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCCTCGTAGTAATAACCACCACT12750.40019962899140277No Hit
AGGGTTCCCTGGCCCCAGATCACCGGTTTATAATAACTCCCCGAACCACA12540.3936081056903679No Hit
AGGGTTCCCTGGCCCCAATAGTCATCTAGTCCGAATATCGACGCCGGCCC12450.3907831671327815No Hit
AGGGTTCCCTGGCCCCAGGACCAGCCACTGCTATAGAGTCTCGCGGTACA12430.39015540300887347No Hit
AGGGTTCCCTGGCCCCAGCCGCCCGGACCATAAACTTCCCTCCCGAAGTC12040.37791400259266583No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC12010.3769723564068037No Hit
AGGGTTCCCTGGCCCCATTGAGTCCAAGAATTGTATCGATCTCTCGCACA11720.3678697766101365No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTGGGGTAGCAATCACCACC11610.3644170739286421No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGGGGCAGCTGGTACTACTACAATA11430.3587671968134693No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTCGGCGGTCCAATCACC11270.35374508382220465No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA11220.3521756735124344No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAAATCCAGTTGTAATAATTCCCCGA11220.3521756735124344No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGTCATCCTCTGGCAGTCACCA11110.34872297083094No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC10760.33773709866254853No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCCGACCAGTCATGCCCACCCAG10660.3345982780430081No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATTTATAGCTGTATCCTCCTCTCGC10600.3327149856712839No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGTAACCACTACTATCATAGTAATAC10410.3267512264941571No Hit
AGGGTTCCCTGGCCCCAGTAGCCAAATCCCCGCATAGCAGCTGGTACTAC10140.31827641082139796No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTACCTCTAACCATAGCTGTATC10070.3160792363877197No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTGAGTGACGAGCTGGGA10040.31513759020185755No Hit
ATTGTCCCTTGGCCCCAGATCGCTGGTACTACTACAATATCGTAGGATTC9730.3054072462812823No Hit
GTGGTCCCTGTGCCCCAGACGTCCATGTAGAAATATCCCGCGTAGTTACT9090.28531879431622365No Hit
AGGGTTCCCGGGCCCCAGTAGTCAAAGTTGGCAGCTGGCATCTGTCTCGC9060.2843771481303615No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAAACCACTCCAGCGCACCCACAAG8980.28186609163472914No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTGGGAATAACCGGTACTATCAGA8920.27998279926300496No Hit
AGGGTGCCCTGGCCCCACACACCCCCGGCAAGATCTGTGGTACAGTAATA8910.2796689172010509No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTCCCAGTAGTATGGCAGATGGAA8890.27904115307714283No Hit
AGGGTTCCCTGGCCCCAGTAAGACATTGGGGATCCCCCGTAGTTCCATGT8840.2774717427673726No Hit
AGGGTTCCCTGGCCCCAGTAGTCCTCGTAGTAATAACCACTACTATCATA8570.2689969270946135No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGATTTAGCAGCTGGTACTACTACAA8550.2683691629707054No Hit
AGGGTTCCCTGGCCCCAGTCCCCAGGCGGTTGGATCCAGTTATACGGCCC8050.2526750598730033No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCGGTCTTCAAATCGTGGCCGCT7780.2442002442002442No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATAACCCGCCCCCAGCTGCTGC7740.24294471595242806No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAACCACCTACACCTGTAAC7030.22065908955369112No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATATACCAGCCCTCCCGCCTCCCCCG6930.2175202689341507No Hit
AGGCTAGGGCCCTTGGTACGGGCTGAGGAGCTCTTTCCTCACTTTCCCTG6870.21563697656242645No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGACCAGCCACTGCTATATCGAG6600.20716216088966732No Hit
AGGGTTCCCTGGCCCCAGGAGTCCAAATAATAACTATTACTATCATATTT6560.20590663264185116No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGACGCTCGTCGTTCCAGTT6540.20527886851794308No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAAATCGTAGTAGCTCCCACCCGCT6370.1999428734647244No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGCCAGCGAATGCTTTCGC6200.19460687841150565No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATAAAAGGACCCCGATTGATC6190.1942929963495516No Hit
AGGGTTCCCTGGCCCCAGTAGCCGAGAAAATCTCTCACACAGTAATATAC6100.19146805779196524No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGCGAGTAATAACCACTACTATCATA6000.18832923717242483No Hit
CAGCCTGGAGTCGCCCATTCCGCCCCCACCAAAGGACCCTGCACCCAGCT5930.18613206273874655No Hit
AGGGTTCCCTGGCCCCAGTAGGGTAATCTGCCCGAATCTCTCGCACAGTA5910.18550429861483847No Hit
AGGGTTCCCTGGCCCCAGTAGTCGTAGCTCCCACCCACTAGATGTTTCGC5900.18519041655288443No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATTTATAGCTGTATCCTCCTCTCAC5710.17922665737575763No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACCCCATATAATAACC5620.17640171881817124No Hit
GCAGGTGGACCCTGCTCTGTGGGAAGCAGCCTGGGGGCTACGGGAGGCAG5450.17106572376495255No Hit
AGGGATCCCTGGCCCCAGAAGTCAAAGTATCAGTTGGTTCTACTAACACA5290.1660436107736879No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCACTTATAGGGTAGCAGCTACC4540.1425024561271348No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTCTATAATAACCACTCCAA4510.14156080994127265No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATCTTCCCGAGCAGCAGCTGGTACTT4510.14156080994127265No Hit
ATTGTCCCTTGGCCCCAGATATCAAAGACATCCCGGTAACCAGTCCAAAA4440.1393636355075944No Hit
ACACGCAGTGTGATATCCTGGGACAGAAGGAGGACGCTGAGGGAAACCCA4410.13842198932173225No Hit
TGCAGGTTACCTGTTCCCACACACTCGCTGGGCCCCAGCAAGGCGCCACG4110.12900552746311103No Hit
TTCCCTCCCTAGACCTTTTCCGAGGGGCAGGTCATGGATGTGAAGTTCCT4110.12900552746311103No Hit
AGGGTTCCCTGGCCCCAGTTCTTGTGGGGAGCGGGCCAATACCCACTCCA4100.12869164540115696No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCGCCGTGACCCCATATCTTTCGC4080.12806388127724888No Hit
CCTGTCCTTCTCACGGGGGGTCTCCAGCACACCCTGCTGCCACTGGCCCC3850.12084459385230595No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTACGGTAA3840.12053071179035188No Hit
TGCTCTGCCAGTAAGGGATGGAGCTGGGCCTCAAGCTATGCTATAGGCAC3830.12021682972839784No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATGGGTTGTAGCCATTCAACATAAG3820.11990294766644381No Hit
CAGTTGGCCAGTAGCCCACCCAGATCTTTAGCTCCAGGCAGGGCCTGACT3780.11864741941862765No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCACATTGTGACAACAA3560.11174201405563873No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTTATGTTCTCCCGAACCATAGTA3530.11080036786977662No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGATTTCAGACAGCAACGG3440.10797542931219024No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA3380.10609213694046599No Hit
GTGGTGGTAAGGGTGTCCAACCCGGCAGAGGCGATGGGCAGACAAAAATG3370.10577825487851195No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGTCGTTCCAGTATTTCTTGCACA3260.1023255521970175No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA3230.10138390601115536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)