FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417538
Sequences flagged as poor quality0
Sequence length300
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA28038467.15173229741963No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACC77861.8647404547610036No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC32950.7891497300844473No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGCACTACTACA18910.45289291034588464No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTCCA16170.3872701406818062No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGCTGGTACTACTACA13470.3226053676551595No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA10910.2612935828595242No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC10670.25554560303493334No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTAATACA9620.23039819130234854No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTAAA8910.21339375098793403No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGCACTACTACC8890.21291475266921814No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTCCTACA8700.20836426864141708No Hit
AGGGTTCCCTGGCCCCAGGGGTCGATCCACCCCATGATGGTACTACTACA8150.1951918148767298No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCACGATGGTACTACTACA7790.18656984513984357No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCCTGATGGTACTACTACA6970.1669309140724916No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGACGGTACTACTACA6890.165014920797628No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAAGGTGTAGCTCCAAAAAT6530.15639295106074177No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTTCTACA6450.15447695778587817No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACCACA6100.1460944872083499No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTATA6080.145615488889634No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGTTACTACTACA5940.1422625006586227No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG5750.1377120166308216No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACT5650.13531702503724213No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTATTACA5270.12621605698164No Hit
AGGGCTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA5030.12046807715704919No Hit
ATTTTTAGCCCTTGGCTAGGTGTGGGGCACATAGAAGGAGCCCCATCATG4780.1144805981731004No Hit
AGGGTTCCCTGGCCCCAGGGGTCGACCCACCCCATGATGGTACTACTACA4680.11208560657952091No Hit
TGGGGACAGGGACAGGTGGTCCTGGGATCAGGCTTCAGCTGCACGTCCTG4560.1092116166672255No Hit
AGGGTTCCCTGGCCCCAGGGGTCGCACCACCCCATGATGGTACTACTACA4550.10897211750786753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)