FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406156
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACGAGCTGCTATT4460210.981494795103359No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAAAGCA390519.614778557992496No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC21680.5337850481095934No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA9870.24301007494657226No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC7430.18293463595268813No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCC6420.15806734358226887No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCG5930.14600301362038232No Hit
GTGGTCCCTTGGCCCCAGACGTCAACAATATAATAACCACTCCAAAAATC4720.11621150493898895No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTGTAGCTCCCACCTCC4690.11547287249234284No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCTTCGCTCAAGATCTCTCGC4600.1132569751524045No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAGGAGCATACACCACC4580.1127645535213071No Hit
ATTGTCCCTTGGCCCCAGACATCAAAAGCATCACCCGAGCCCGAATAACA4560.11227213189020967No Hit
GGGGTTCCCTGGCCCCAGGGGTCGAATCAGTTGTCCCATTGAGACAACTG4470.11005623455027133No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGCCGTTCGAGCT4460.10981002373472261No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTGGAGGCA4430.1090713912880765No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATCTCGGGTAGTTTGGTGATCCAGC4410.1085789696569791No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAGTAGTAGTAAGTAGTACT4340.10685549394813816No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATTG4340.10685549394813816No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCCTTAACCACTCC4340.10685549394813816No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCGTCGTAGTCACCGTAGTC4310.10611686150149205No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGCCGTAGTGGTAGTTGGTCCAACT4270.10513201823929721No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACCAGCCACTGCTATAAGA4260.1048858074237485No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGGGGCC4260.1048858074237485No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTCGAGCT4240.1043933857926511No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATATTGCTCCCTGAACCATGTCTG4240.1043933857926511No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGGGTCCA4220.1039009641615537No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGGG4210.10365475334600499No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGTTCTG4200.10340854253045627No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGACGGAGGCCACTGCTATA4200.10340854253045627No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCGGTCCAAAAATCGTAAA4170.10266991008381016No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTTAGACGAGCTGCTATA4130.10168506682161534No Hit
GCGGTCCCTTGGCCCCAGAGGTCCATACCAAAGGCCTCCATAATTACCAC4110.10119264519051793No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCCATCCCCTTATA4090.10070022355942053No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGAGCGGAGTGAGCTGCTATACTCCG4090.10070022355942053No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATCCCTC4080.10045401274387182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)