FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423368
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC61881.461612592354642No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA15180.3585533153190605No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG9980.23572872772623346No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGGG8320.19651934014852326No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGACGTAGT7430.17549743957975095No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCTCAAGATAGCTCCCACCACG6960.16439598647039927No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGCGGA6680.1577823548307855No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCCCAGTTACTGTAGTCATG6320.14927911415128212No Hit
GCGGTCCCTTGGCCCCAGACGTCCAAAGCGTAGTAGTAGAAGTGTCTGAC6300.14880671189130967No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGAGGATTGTAGCCGCTCTCGC6170.14573609720148903No Hit
ATTGTCCCTTGGCCCCAGAAATCAAAAGCAAACCCCGTAGTTTGGCAGCT6070.14337408590162695No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCGGATTAC5940.14030347121180628No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATTG5930.14006727008182007No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACTGAACCATAACCCCTGAAACTCT5900.13935866669186145No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCCCTTTGA5810.1372328565219856No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCAGAGCAGCTGGTACTACT5790.1367604542620132No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCATAACTCCCTGAACCATA5760.13605185087205457No Hit
AGGGTTCCCTGGCCCCAGTAGTCCGCGCAGTCACCACCACAATTGAGGTC5730.13534324748209595No Hit
GTAGTCCCTTGGCCCCAGACATCCATACCGTAGAAATAACTATTCCAAAA5690.13439844296215112No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCTACCCGACGAAGGACTCCCCG5630.1329812361822339No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGTAGTCTGTG5630.1329812361822339No Hit
AGAGTTCCCTGGCCCCAGTAGCCGTAGTTCCAGTTGAAATGTCTCGCACA5580.1318002305323029No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCGGCTGCCGTAATCTCTCGC5540.13085542601235803No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCGTAGCAGCTACCACCACT5530.13061922488237185No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCACCCCCGGAATCGTAACGTCG5500.12991062149241323No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCCGTGGGCA5500.12991062149241323No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGATTAGC5490.12967442036242702No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGATTTCTCC5480.1294382192324408No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTGTAATCGTA5450.1287296158424822No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTCAAAAGGACT5440.128493414712496No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCGTATAGCAGTGGCTGGTA5440.128493414712496No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCAGGCCCCGTAGCTCCCACA5340.12613140341263393No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAATGTT5330.12589520228264772No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAATCCCGCAGCTGGTACT5330.12589520228264772No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATCCGCCACTGCTATACGCTACTCT5320.12565900115266151No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCAACCGT5290.12495039776270289No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCCCGAACCATAGTAAT5270.12447799550273048No Hit
GTGGTCCCTTGGCCCCAGACGTCCTTTCCACTGCTATACCGACGATCTCT5270.12447799550273048No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGAGAAATTGTAGCCATCTCTAGA5250.12400559324275807No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTATCGGTCAGTCGTTATCTC5220.12329698985279947No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAAAACGGGTTCTCGCACAGTAATA5200.12282458759282705No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAGTCTCAACGTCACCCA5180.12235218533285462No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCCGGCCAGTCATC5180.12235218533285462No Hit
AGGGTTCCCTGGCCCCAGTAGTCATAATTGTAGCCATCTCTACCCTCTCT5170.12211598420286843No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACGTTGAGGCGTGATC5160.12187978307288223No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGACGGACGAGCTGCTCTCTACTGTG5150.12164358194289601No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCGGTAGT5140.12140738081290982No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCTCAAGTTCGG5100.12046257629296497No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGTAA5090.12022637516297878No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATACCCGGGAGATTTACCGTA5090.12022637516297878No Hit
AGGGTTCCCTGGCCCCAGTAGTCCAAAGGTAGCCACTCCTCATCTCTCGC5080.11999017403299259No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCAGCTGCTGCTAT5060.11951777177302016No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCCGTCGGTAGTTACTGTAGG5050.11928157064303396No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCACTAATTGT5050.11928157064303396No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATAT5020.11857296725307535No Hit
AGGGTTCCCTGGCCCCAGAGGTCCGCCCCAAATGTTCCTCGCGCACAATA5010.11833676612308913No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTGATACGAGAGTAGCAGCT5000.11810056499310292No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCTCTCATCACATCTCTCGC4990.11786436386311673No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTGCGTAGAGCCCCGAACCCGAACC4980.11762816273313051No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAACCAC4960.1171557604731581No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACGGGAAAAAGAG4930.11644715708319947No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCTAAAACCGAAATAATAACC4930.11644715708319947No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTGGGCAG4920.11621095595321328No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGCCCATCGA4920.11621095595321328No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAACCGGT4900.11573855369324086No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAATAAGTCCCCGAACCACCGTA4890.11550235256325467No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATAGTTCCGGCCCCTTCTCTCGCACA4850.11455754804330985No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCTTCAGTCAA4850.11455754804330985No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGAATTGCCCAGTACCA4850.11455754804330985No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGGCCTATA4840.11432134691332363No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGTCTCC4840.11432134691332363No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTTTGTCTATAGCAGCT4830.11408514578333744No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATAACCTCCCGAGCCGGCCAA4830.11408514578333744No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGACTTAGCAGCT4790.11314034126339259No Hit
GTGGACCCCTTGCCCCAGACGTCCATGTAGTAATAAAAAGAGGATCCGCC4790.11314034126339259No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGGCCGCTTCCT4780.1129041401334064No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTGCTTTCTCCGATAAC4770.1126679390034202No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGAGAGGGTTATAATA4770.1126679390034202No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGAGTAGTCGTAATAACC4770.1126679390034202No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCATGTAACTCCCG4770.1126679390034202No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGAACGGGTTCGACTCAACCTTAAT4760.11243173787343398No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCCGTAACCATAGCTGTATCCAC4750.11219553674344779No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTAGAGTAGCAGTTACCATA4740.11195933561346158No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCTACGAGCTGCTATATTCGA4740.11195933561346158No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC4730.11172313448347536No Hit
AGGGTTCCCTGGCCCCAATAGGCAAGGTCGTACCAACTGCCGCTATACTC4730.11172313448347536No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGTGATAA4730.11172313448347536No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCGACAGCCACT4720.11148693335348916No Hit
GTGGTCCCTTGGCCCCAGACGTCCAGACCGATGTACTTCGCGGGCATGCG4720.11148693335348916No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACTTGACGAGCTGCTATCTAGGGG4720.11148693335348916No Hit
GTGGTCCCTTTGCCCCAGACGTCCATAGCGCAAAAATAGGACGAAGTAAC4710.11125073222350297No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCAGCTACCACCACTACA4710.11125073222350297No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTCTTGCC4700.11101453109351675No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAGATGGG4690.11077832996353054No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAATAGTAGACGTTGTAGCC4680.11054212883354433No Hit
AGGGTTCCCTGGCCCCAGAGCTCCCCGAACCATAGTTTATCTCTCGCACA4680.11054212883354433No Hit
AGGGTTCCCTGGCCCCAGTAGTCCACGGACGAGCTGCTCTTTGATCTCGC4680.11054212883354433No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAATTCACGTA4680.11054212883354433No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGTAGCAG4660.11006972657357193No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGGTTTGTAATAACCACT4660.11006972657357193No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCCTAGTAACCCACTACTATC4650.10983352544358571No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGGCA4640.10959732431359952No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGGGGAATGACCACT4630.10936112318361332No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATGG4620.1091249220536271No Hit
AGGGTTCCCTGGCCCCAGTAGCCATTTCCAGTCACTGCTATGCCCGGGAG4620.1091249220536271No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATCCGCCATCGGAAGAGTA4610.1088887209236409No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGAGACCGAAGGAGTTACCACCGTC4600.1086525197936547No Hit
GTGGTCCCTTGGCCCCAGACGTCCATCGGCCTAAATAACCATAGCTGTGC4600.1086525197936547No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG4600.1086525197936547No Hit
AGGGTTCCCTGGCCCCAGGATACAGCATACGCCATTTCTGGCCCGCTCGC4600.1086525197936547No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGGGGGATCCCCCAGTTAA4590.10841631866366848No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTGGAGAGACCAGTCAAAAT4580.10818011753368229No Hit
AGGGTTCCCTGGCCCCAGTCGGGGCAGCTACCACCACCCTTCTCCTCTCT4580.10818011753368229No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAAAGTT4580.10818011753368229No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAACCGTAGTAGTACCGCATAACTCC4570.10794391640369608No Hit
AGGGTTCCCTGGCCCCAGTTCACATAACCACTCCAAAAATCATCAACTTG4560.10770771527370987No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCCACCACCCGCTTCCCAATCTCTCGC4540.10723531301373747No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCATGTC4540.10723531301373747No Hit
AGGGTTCCCTGGCCCCAGTAATCTAAGGGGTTCACCGTAGCCCTAATCGC4520.10676291075376505No Hit
AGGGTTCCCTGGCCCCAGTAGCCGACCATACCACTACTACCCGCATTTAA4520.10676291075376505No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTGAGACACTCCAAA4510.10652670962377883No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGATTCCA4500.10629050849379264No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGCCGTAGTAGTGGTATCTACCCCT4490.10605430736380643No Hit
AGGGTTCCCAGGCCCCAGTGGTCTATCAACCACCCGGCGCCCGCCGCACA4480.10581810623382021No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAGAGACGCCAGCCACTGCT4480.10581810623382021No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAGGATAAAGTAGCAGCT4480.10581810623382021No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCAGCAAACAGACTCCCT4470.10558190510383401No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATGAGGCCAGCCACTGGTC4470.10558190510383401No Hit
TGGGGACAGGGACAGGTGGTCCTGGGATCAGGCTTCAGCTGCACGTCCTG4460.10534570397384782No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCCTCCGGACGTAGTCACC4460.10534570397384782No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGAGTGTAGGGATAACCAGTCAAA4450.1051095028438616No Hit
GTGGTCCCTTGGCCCCAGACGTCCAGATCGTAGTAGTATGTGGCGGAGTA4450.1051095028438616No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAACCCCTAAG4450.1051095028438616No Hit
GTGGTCCCTTGGCCCCAGACGTCCCAACCTCTCGCACAGTAATACACAGC4440.1048733017138754No Hit
ATTGTCCCTTGGCCCCAGATATGAAAAGCGTCATCCCACCTAATAACCTT4440.1048733017138754No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTCGATATAGCAATCACCACG4440.1048733017138754No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGTAATTTCAGATCTCT4430.1046371005838892No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAAAAGGCC4410.10416469832391678No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATGGGAT4410.10416469832391678No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCTATAGCATACACCATT4390.10369229606394437No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATCTATGGC4380.10345609493395817No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAATTGTAGCCATCTCTACCCCAGTG4380.10345609493395817No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTCCCCGAACCAGCCAC4360.10298369267398574No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGGGGATCTGGAATGGGGCGGATC4350.10274749154399955No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGCG4350.10274749154399955No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATTAGGCCCGACAGTCCGTGCCCTCT4350.10274749154399955No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAAAGGAAAAAGTTACTTT4340.10251129041401333No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCCCC4340.10251129041401333No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGACGACCATAGCTGTATCCCTTTG4340.10251129041401333No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCCCCCGTACCAGCTGCTGC4330.10227508928402713No Hit
GTGGTCCCTAGGCCCCAGACGTCCATATCGTAGTAGTAGCACCGTTGTAA4330.10227508928402713No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTAGCGGCCCAACTGAT4320.10203888815404094No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGAGGTTATCCGTGC4320.10203888815404094No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGAATGAGCTG4310.10180268702405472No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAATCGGCATAAGC4300.10156648589406851No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCTTGAGAAGCTGCTGCTAT4290.10133028476408232No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCTTTGTCTCTCGCACAGTA4290.10133028476408232No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATTTTATAGC4290.10133028476408232No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTATTGGATTTGAGG4270.1008578825041099No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCACGGG4250.10038548024413749No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTCCGGTA4250.10038548024413749No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCCGT4240.10014927911415128No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAACTACGTA4240.10014927911415128No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCGCCCC4240.10014927911415128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)