FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378452
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG8158821.558348218532338No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACATTGTTGCGCCTGATCCTACTAG8530.2253918594696289No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATGG5990.15827634680223648No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAACTTGTTGCGCCTGATCCTACTAG5730.15140625495439317No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCGGCAGCTACCACCACT5000.13211715092006385No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACCAGCT4900.12947480790166255No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAACCCCCTCGACTCCTCACACCGTC4770.1260397619777409No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGCGAAGTAGCTCCCAC4600.12154777884645875No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGGCTTC4560.12049084163909822No Hit
AGGGTTCCCTGGCCCCAGTAGTCTGGATAACCACTACTATCATAGTAATC4500.11890543582805746No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTATAATAACTCCCCGAACC4420.11679156141333645No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCGCTCGG4370.1154703899041358No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCACGGCCGGTACCAGCTGCT4370.1154703899041358No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCGGCTCGACCCGA4310.11388498409309503No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCAACGTAGTCACCGTAGTCCCC4240.11203534398021413No Hit
GTGATCCCTTGGCCCCAGACGTCCAGAGCGTAGTAGTAAGCGTAGCCCTG4200.11097840677285363No Hit
ATTGTCCCTTGGCCCCAAATATCAAAACCAACCTCGTAGTAATAACCACT4180.11044993816917337No Hit
GTGGTCCCCTGGCCCCAGACGTCCATACTGTAGAAGTAGGGTGTAGTCAA4140.10939300096181287No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCTCTCCGAACCGCTCT4130.10912876665997273No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGGTAACC4110.10860029805629247No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTCCCCATTACCACCGT4100.10833606375445234No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCCCGTAGCTCCCGCCTAT4100.10833606375445234No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTCTCGTCATAGGCCCTTTTCGC4070.10754336084893197No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGACCGAATAGCAGCTGGT4070.10754336084893197No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGGAGTAATAACCACTACTATCATA4060.10727912654709183No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATAACGTCACCGTAACCTCGTCTCGC4060.10727912654709183No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAAGTAGTAGTAAGAGCC4050.10701489224525171No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCTTCATA4040.10675065794341158No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCAGCTACCACC4030.10648642364157145No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATACCCAGCCACTGCTAT4020.10622218933973133No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAGCCTCCCAG4010.10595795503789121No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATTGTAA3980.10516525213237081No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCTCACAGTAGG3920.10357984632133005No Hit
GCGGTCCCTTGGCCCCAGACGTCCATGCCGTTGTAAAATTGGTTTGCCTG3920.10357984632133005No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGCCCGAGTAGCAGCT3890.10278714341580968No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGCCTCCGTGGCGGCGTAGCCACT3860.10199444051028929No Hit
AGGGTTCCCTGGCCCCAATAGTCATTCCATCCCAGATCATTATAGTACGT3850.10173020620844915No Hit
GTGGTCCCTTGGCCCCAGACGTCGAAACCAACTTCTCCGTAGCCATCTCT3840.10146597190660903No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCTGTCTATCGTAACATG3830.10120173760476889No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAACCGATACCACCATCTTGCTT3810.10067326900108865No Hit
GTAGTCCCTTGGCCCCAGATGTCCATACCGTAGTAGTGGTAGGTACCCCC3800.10040903469924851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)