FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364071
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACGTAGACATCTCCAAATAACT5930.16288031730074629No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC5780.1587602418209635No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCACCTCAACCCCAAATAG5680.1560135248344416No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAATAGTAGGGGTACATGTTGTT5600.15381615124522413No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAAGTCTCCACTCTCGCACAGTA5540.152168121053311No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCGATCCAGCGG5510.15134410595735448No Hit
GTTATCCCTTGGCCCCAGACGTCCATGCCGTAGTAGTCGTAGCAGCTGGT5420.1488720606694848No Hit
AGCGTTCCCTGGCCCCAGTAGTCAAGATAGTAATTCTTCAAATCAGAGTT5320.14612534368296293No Hit
TGCATGCCCTGACCCCAATAGTCAAAGTAGTAGTGATAAACAGCCGGCAC5190.14255461160048452No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCATAGTAAACCTTCAC5140.14118125310722357No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATGT5090.13980789461396265No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGACAGGCCCGAGGCGCCCCCT4930.13541314743552768No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATCGACCAT4930.13541314743552768No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGCCACATAACTCCCCGAACC4910.13486380403822332No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGCTCGATGGGATTGTAGCCATCCC4900.13458913233957115No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCAAAATAACCACTACTATC4860.13349044554496237No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTGTACCAGCT4840.132941102147658No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAACGTAGTTGTAGTATCCCCTGAG4820.13239175875035364No Hit
AATGTCCCTTGGCTCCAGATATCAAAAGCATCATGGTAGTCAGCCGCTGC4780.13129307195574488No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCGAGCCCCT4770.13101840025709272No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAATCCAGCCACCATTCCTC4760.13074372855844052No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGTGAGGTGACCAGTCAAAATATC4760.13074372855844052No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAGTATGACGAACCACCTCT4740.13019438516113616No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAATCCACCTAT4740.13019438516113616No Hit
AGGGTTCCCTGGCCCCAGTAGTCATAACCACTACTATCATAGTAATACGG4740.13019438516113616No Hit
GCGGTCCCTTGGCCCCAGACGTCCATATTGTAGTTAAGAATGTCCCGAAG4700.1290956983665274No Hit
AGGGTTCCCTGGCCCCAGTACTCCAACTGGGAGCTCCCAGTTTCCCTCGC4700.1290956983665274No Hit
AGGGTTCCCTGGCCCCAGTAGTCGGTGTACCAGCCCCGGAGCTCTCTCGC4680.12854635496922304No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAAGGTAGCCACTATATCGCTTCTCGC4680.12854635496922304No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGCCTTTGGACGAGCTGC4670.12827168327057087No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGACCCCCATCCACAGCTATACCGGG4650.12772233987326648No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAATCCATAACGGG4630.1271729964759621No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACGAGCGGTGGGGGCCAGC4630.1271729964759621No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCAGCTGGT4590.12607430968135336No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTGGGGTGGATAGTCCTG4580.1257996379827012No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTATTGAACCGTACCAGCCACCAG4580.1257996379827012No Hit
GTGGTCCCTTGGCCCCAGACGTCCATCTTCGATCTGGTCGCACGAGCTGC4550.12497562288674462No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTACCAACCAAACGT4540.12470095118809242No Hit
AGGGTTCCCTGGCCCCAGTACTCAATAACTCCCCGAACCATAGTATCCCA4530.12442627948944025No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCCCGGCCACCCCGTAGCA4530.12442627948944025No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCATCCCTAGATAG4500.1236022643934837No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTGGGACATAGT4490.1233275926948315No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAAGAGA4490.1233275926948315No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTCAAGGTTACTGTAAGT4480.12305292099617932No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGACCGACGTAGTAGCTCCCAC4460.12250357759887494No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGTTTCCCAAACGTACCAGCCACT4460.12250357759887494No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGCCAATAACTCCCTGAACC4410.12113021910561401No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCCAACCTTAATAACTCC4390.12058087570830964No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACGGAATCGTTACCCCTCT4380.12030620400965744No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTCGAACGGTAT4370.12003153231100527No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAACGTGTAGTA4360.11975686061235309No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATAGGGA4350.1194821889137009No Hit
ATGGTCCCTTGGCCCCAGATATCAAAAGCATGCCAACTGTCGCTACCCTT4320.11865817381774434No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGAGTAGTCATCTGG4310.11838350211909214No Hit
AGGGTTCCCTGGCCCCAGTAGCCGAACGGGTTTAGCACCGGAAACTTCGG4290.11783415872178779No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATCTCTCGCACAGTA4290.11783415872178779No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCTTCCG4270.11728481532448341No Hit
AGGGTTCCCTGGCCCCAGTTGTCAATTTGGCCGGTCTTCGCACAATAATA4260.11701014362583123No Hit
AGGGTTCCCTGGCCCCAGTAATCAAGATGCCTATAATTACTATTATCATC4240.11646080022852684No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGAAATA4240.11646080022852684No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCTAACTCCCCGAACCATCC4230.11618612852987466No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGAATAGATGTCTCGCACAGTA4230.11618612852987466No Hit
AGGGTTCCCCCCCGCCGGGTCTCTTGCACAGTAATACGCAGCCGTGTCCC4210.11563678513257031No Hit
AGGACTCCCTGGCCCCAGTAGTCAAAGTAGTCCGAGCTGCTCCCCCTACC4200.11536211343391811No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCGGGCCCCCAAATATAGCA4180.11481277003661373No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCAGTCCGCCAAGTATAGCA4170.11453809833796155No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAAATAACATATAACTCCCC4160.11426342663930936No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAATAACCACTACTATCTCTCGC4160.11426342663930936No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCTACAAT4140.113714083242005No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCGGCTGTTACCACCGTGCCCCC4140.113714083242005No Hit
AGGGTTCCCTGGCCCCAGTACTCCAACGGGGCCACTCCAAAAATCGTACC4130.11343941154335281No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTGACCAAG4130.11343941154335281No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCACCCTATTGTATTATCATA4100.11261539644739625No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAGGCCGAGTGAGGTCGATCTCTTAC4100.11261539644739625No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTGGGTACCAGCCACTGCCGT4090.11234072474874406No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTTCTTGCC4090.11234072474874406No Hit
AGGGTTCCCTGGCCCCAGTAGTTTGACGAGCTGCTATACCCGAGATCTCT4080.11206605305009187No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATTTGGTCCAGTCAA4050.11124203795413533No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACATAATGTCCCAATT4040.11096736625548313No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCAGGCGACGAGCTGCTATAAGT4030.11069269455683095No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGGGA4020.11041802285817875No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCCGTAGTACCAGCCAC4020.11041802285817875No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGTTTACCGTACCAGCCACTGCT3990.10959400776222221No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCGACCAGCCA3980.10931933606357003No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATCCGTACCAGCTGCTGCGCGGATC3980.10931933606357003No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTTTTTGTGATCGCCCGGGTCCCCC3970.10904466436491783No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAAATCGTAC3960.10876999266626565No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCATCGTA3950.10849532096761345No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTACTTTGA3950.10849532096761345No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAACCAGT3950.10849532096761345No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCGTTTTTT3930.10794597757030908No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACCTTCCGGAGTTACCACCGTAGCC3920.10767130587165691No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGACGACCCC3920.10767130587165691No Hit
ATTGTCCCTTGGCCCCAGATGTCCGGGGGGGTCCAGCCACTACTATATAG3920.10767130587165691No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACGGCGTACAGTAATA3910.10739663417300473No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGAAGTGGTACGTCCCCAGCCG3900.10712196247435253No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCCCTTGTGCC3900.10712196247435253No Hit
GTGGTCCCTTTGCCCCAGACGTCCAGGGCGCCGCCGTACCAGCTGCTGCT3900.10712196247435253No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGAGTAGCT3900.10712196247435253No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTATTAACCACTACTATCAT3900.10712196247435253No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAGGCGGACGTAATAACC3900.10712196247435253No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAAGTAGGCCAGCCACTGCTATA3890.10684729077570035No Hit
GTGGTCCCTCGGCTCCAGACGGCCATATAATACATGCAACATTAAAGGGG3890.10684729077570035No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAACCTAGATACGATCCACCCCA3880.10657261907704815No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGCCT3880.10657261907704815No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGTCTTTTCACGACCAGT3860.10602327567974378No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGCGTCGATAATAACCAGT3860.10602327567974378No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATCCGGTATTATTACAATACT3850.1057486039810916No Hit
GTGATCCCTTTGCCCCAGACGTCCATGTAGTAGGCGTAGTCCCATACATC3850.1057486039810916No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAAGGGTTTAACTGTAG3840.10547393228243943No Hit
AGGGTACCCTGGCCCCAGTAATATGGGGAACCACTCCAAAAAGAATCTTT3830.10519926058378723No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGTAGTAGTAGCCACCATGGTT3830.10519926058378723No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATACCAGTGTCCCCTTGCACA3830.10519926058378723No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTGACCCTAACTATGCCTAA3830.10519926058378723No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAAGGGCAGC3830.10519926058378723No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGGACGCAAAGTAGCTCCCACT3830.10519926058378723No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGTTTTAGCAGC3820.10492458888513505No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTGGACCACTC3810.10464991718648285No Hit
AGGGTTCCCTGGCCCCAGTAGGGAAAAAGGGCCTCATAGCAGTCGATAGT3810.10464991718648285No Hit
TGGGTTCCCTGGCCCCAGTAGTCTCTCCCGACAACAACTCCCCGACCCAC3800.10437524548783067No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGAAGTAGAAGTAGCGTGACACACA3800.10437524548783067No Hit
AGGATTCCCTGGCCCCAGGGGTCGAACCACCTGAGGGATTCACTCCTAAA3790.10410057378917847No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCTGTCCCAAACGCGGAAAC3790.10410057378917847No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCAAGCTAGCCCGGG3770.1035512303918741No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAATGAAGGTACCAGCCACTCTCCAT3770.1035512303918741No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACGACGCGCTACTATACGG3770.1035512303918741No Hit
AGGATTCCCTGGCCCCAGTAGTCAAAATACCCCCAACTGCCAGCTGCTGT3770.1035512303918741No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCCCTTTCCCCCGTAGTC3760.10327655869322193No Hit
ATTGTCCCTTGGCCCCAAATATCAAAAGCATCATGGTCGGCGGTAGCTTT3750.10300188699456975No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATAAGTAGC3740.10272721529591755No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATGTTGCCA3740.10272721529591755No Hit
GTGGTCCCTTTGCCCCAGACGTCCATCCCCCTCAGGAATCTCGCACAGTA3740.10272721529591755No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAAGGAGTA3720.10217787189861317No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCAGCCTTTGT3720.10217787189861317No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAATCACCCGGACGAGCTGCTAT3710.10190320019996099No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCGCACAACCCTCCGGTA3710.10190320019996099No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTCCTCCCTTCTCTTGC3700.1016285285013088No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCTCGCGTAG3700.1016285285013088No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAATAAAACCAC3690.10135385680265663No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTTTCGTG3690.10135385680265663No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAACCTCGTACCAGCTGCTGCTATA3680.10107918510400445No Hit
GTGGCCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGGAAAGAAA3670.10080451340535226No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATTGGACCA3670.10080451340535226No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGTCGTAGTCACCGTAGTC3660.10052984170670007No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAAATTTTAACAGCTGGTACTACGTG3660.10052984170670007No Hit
GTGGTCCCTTGGCCCCAAAACGAACCGTTACCGGTATCTCTCGCACAGTA3660.10052984170670007No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAGAAGTACTGATAACTCCCCCGAAC3650.10025517000804787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)