FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420798
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC7762218.446380448576278No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGACCAG236125.611243399445815No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC70231.6689718107025224No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA18910.44938426513434No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG15170.360505515710626No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC9480.22528624185476168No Hit
ACAGACAGGCCACCCAGGGCTCAGGCTGGGTATGGTGGGCTCTGGTGGGA6330.1504284716182111No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCGCGGGTAGCCGGGTCCAT5250.12476295039425091No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGGTTTCATAACTCCCCCA5150.12238651324388425No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGACCCG5040.1197724323784809No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG4830.11478191436271085No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGAAACCCGACGAGCTGCTATA4770.11335605207249083No Hit
TGGGGACAGGGACAGGTGGTCCTGGGATCAGGCTTCAGCTGCACGTCCTG4730.11240547721234416No Hit
GTAGTCCCGTTGCCCCAGACGTCCATGTAGTAGGAGACGTCACCGTAACG4710.11193018978227084No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCACCCTGAGCGGA4690.1114549023521975No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCTTGTACCAGCCACTGCG4560.10836553405672081No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA4500.1069396717665008No Hit
AGGGTTCCCTGGCCCCAGTATTCAAAGTAAGAGTAACTCCCAGATTCTCT4450.10575145319131746No Hit
ATTGTCCCTTGGCCCCAGAAATCAAAAGCACCACCGAAAACCATTAATGT4360.10361265975598742No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACATAGCTGTATC4340.10313737232591409No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGATAAC4320.10266208489584078No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGAATA4300.10218679746576743No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAAAATGAACGGGA4300.10218679746576743No Hit
AGGGTTCCCTGGCCCCAGTAGTCTAAGTCACCGTAGCGGTTCAAACTTCC4290.10194915375073076No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCCCCCGTTTCGTACCAGC4210.1000480040304374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)