FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653501
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACGAGCTGCTATT6850910.483381050679341No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAAAGCA603699.237782344632985No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC33220.508338931386486No Hit
GTGGTCCCTTTGCCCCAGACGTCTATGTAGTAGTAGGACGAGCTGCTATT24530.37536285330856417No Hit
GTGGTCCCTTGGCCCCAGACGTCTATACCGTAGTAGTAGTAGGTAAAGCA21270.32547769628508605No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA14390.22019859189197877No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC9120.13955602210249105No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCC9120.13955602210249105No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCG8620.1319049243995036No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTGTAGCTCCCACCTCC7380.11293020209609472No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCCTTAACCACTCC7350.11247113623391548No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACTCGTATCGATAATAACCACTACT7150.1094106971527205No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTGGAGGCA6950.10635025807152552No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCTTCGCTCAAGATCTCTCGC6940.10619723611746577No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCGTCGTAGTCACCGTAGTC6920.10589119220934629No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTTTCGAT6900.10558514830122678No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAACCGACGACCGT6860.10497306048498779No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATTG6830.10451399462280853No Hit
GTGGTCCCTTGGCCCCAGACGTCAACAATATAATAACCACTCCAAAAATC6790.10390190680656954No Hit
GGGGTTCCCTGGCCCCAGGGGTCGAATCAGTTGTCCCATTGAGACAACTG6770.10359586289845003No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGAGCTCCTAGTAGCTCC6730.10298377508221104No Hit
ATTGTCCCTTGGCCCCAGACATCAAAAGCATCACCCGAGCCCGAATAACA6670.10206564335785255No Hit
AGGGCTCCCTGGCCCCAGCGGTCCAAAAATCCCCAGGGCCCTCTCGCACA6620.10130053358755381No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAGGAGCATACACCACC6620.10130053358755381No Hit
GCGGTCCCTTGGCCCCAGAGGTCCATACCAAAGGCCTCCATAATTACCAC6540.1000763579550758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)