FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629507
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAAGTCTCCACTCTCGCACAGTA10110.16060186781084246No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATGT9720.15440654353327288No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCACCTCAACCCCAAATAG9580.15218258097209403No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACGTAGACATCTCCAAATAACT9290.14757580138108076No Hit
GTTATCCCTTGGCCCCAGACGTCCATGCCGTAGTAGTCGTAGCAGCTGGT9270.14725809244376947No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGCCACATAACTCCCCGAACC9020.14328673072737874No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGCTCGATGGGATTGTAGCCATCCC8760.13915651454233233No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCATAGTAAACCTTCAC8710.1383622421990542No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAATAGTAGGGGTACATGTTGTT8710.1383622421990542No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGAGTAGTCATCTGG8550.1358205707005641No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAACGTAGTTGTAGTATCCCCTGAG8520.1353440072945972No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCGATCCAGCGG8480.13470858941997468No Hit
AGGGTTCCCTGGCCCCAGTAGTCATAACCACTACTATCATAGTAATACGG8380.13312004473341837No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCAAAATAACCACTACTATC8360.13280233579610712No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAATCCAGCCACCATTCCTC8080.12835441067374947No Hit
AGGGTTCCCTGGCCCCAGTAGTCGGTGTACCAGCCCCGGAGCTCTCTCGC8040.12771899279912693No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGACCGACGTAGTAGCTCCCAC8000.12708357492450442No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTGTACCAGCT7950.12628930258122625No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAGTATGACGAACCACCTCT7940.12613044811257063No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTGGGGTGGATAGTCCTG7890.12533617576929248No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTATTGAACCGTACCAGCCACCAG7890.12533617576929248No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATCGACCAT7790.12374763108273619No Hit
GCGGTCCCTTGGCCCCAGACGTCCATATTGTAGTTAAGAATGTCCCGAAG7760.12327106767676928No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCAGCTGGT7680.12200023192752422No Hit
AGGGTTCCCTGGCCCCAGTAATCAAGATGCCTATAATTACTATTATCATC7670.12184137745886861No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCCCGGCCACCCCGTAGCA7660.12168252299021298No Hit
AGGGTTCCCTGGCCCCAGTACTCCAACTGGGAGCTCCCAGTTTCCCTCGC7650.12152366852155735No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGCCTTTGGACGAGCTGC7620.12104710511559044No Hit
AGCGTTCCCTGGCCCCAGTAGTCAAGATAGTAATTCTTCAAATCAGAGTT7580.12041168724096793No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAATCCACCTAT7580.12041168724096793No Hit
AGGGTTCCCTGGCCCCAGTAGTTTGACGAGCTGCTATACCCGAGATCTCT7560.12009397830365666No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGTTTCCCAAACGTACCAGCCACT7560.12009397830365666No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTGGGACATAGT7540.11977626936634542No Hit
AATGTCCCTTGGCTCCAGATATCAAAAGCATCATGGTAGTCAGCCGCTGC7530.11961741489768977No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCAGTCCGCCAAGTATAGCA7530.11961741489768977No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCGGGCCCCCAAATATAGCA7520.11945856042903415No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCCAACCTTAATAACTCC7470.11866428808575599No Hit
AGGGTTCCCTGGCCCCAGTAGCCGAACGGGTTTAGCACCGGAAACTTCGG7460.11850543361710036No Hit
AGGGTTCCCCCCCGCCGGGTCTCTTGCACAGTAATACGCAGCCGTGTCCC7440.1181877246797891No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAATCCATAACGGG7430.11802887021113348No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAAGGTAGCCACTATATCGCTTCTCGC7370.1170757433991997No Hit
TGCATGCCCTGACCCCAATAGTCAAAGTAGTAGTGATAAACAGCCGGCAC7360.11691688893054407No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAAGGGTTTAACTGTAG7350.11675803446188843No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTGGACCACTC7330.11644032552457716No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAAGGGCAGC7330.11644032552457716No Hit
AGGGTTCCCTGGCCCCAGTAGGGAAAAAGGGCCTCATAGCAGTCGATAGT7300.11596376211861029No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCGAGCCCCT7280.11564605318129902No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCTTCCG7240.1150106353066765No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAATCACCCGGACGAGCTGCTAT7230.11485178083802086No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTACTTTGA7200.11437521743205398No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATCTCTCGCACAGTA7130.11326323615146457No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGACCCCCATCCACAGCTATACCGGG7100.11278667274549767No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGTGAGGTGACCAGTCAAAATATC7090.11262781827684203No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGCCAATAACTCCCTGAACC7060.11215125487087514No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACGAGCGGTGGGGGCCAGC7050.11199240040221951No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAAGAGA7040.11183354593356387No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTCAAGGTTACTGTAAGT7020.11151583699625263No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCGCACAACCCTCCGGTA7020.11151583699625263No Hit
ATTGTCCCTTGGCCCCAGATGTCCGGGGGGGTCCAGCCACTACTATATAG7000.11119812805894136No Hit
GTGGTCCCTTTGCCCCAGACGTCCAGGGCGCCGCCGTACCAGCTGCTGCT6990.11103927359028573No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAACCTAGATACGATCCACCCCA6980.11088041912163009No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAACGTGTAGTA6970.11072156465297446No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGTTTACCGTACCAGCCACTGCT6960.11056271018431885No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACGGAATCGTTACCCCTCT6960.11056271018431885No Hit
AGGGTTCCCTGGCCCCAGTACTCAATAACTCCCCGAACCATAGTATCCCA6950.11040385571566322No Hit
AGGATTCCCTGGCCCCAGGGGTCGAACCACCTGAGGGATTCACTCCTAAA6920.1099272923096963No Hit
GTGGTCCCTTGGCCCCAGACGTCCATCTTCGATCTGGTCGCACGAGCTGC6900.10960958337238506No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACCTTCCGGAGTTACCACCGTAGCC6890.10945072890372944No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAAATCGTAC6870.10913301996641817No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATCCGGTATTATTACAATACT6860.10897416549776252No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTGACCAAG6860.10897416549776252No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCAGGCGACGAGCTGCTATAAGT6800.10802103868582874No Hit
AGGGTTCCCTGGCCCCAGTTGTCAATTTGGCCGGTCTTCGCACAATAATA6790.10786218421717313No Hit
GTGGTCCCTTGGCCCCAAAACGAACCGTTACCGGTATCTCTCGCACAGTA6790.10786218421717313No Hit
AGGACTCCCTGGCCCCAGTAGTCAAAGTAGTCCGAGCTGCTCCCCCTACC6780.1077033297485175No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGACAGGCCCGAGGCGCCCCCT6770.10754447527986186No Hit
AGGGTACCCTGGCCCCAGTAATATGGGGAACCACTCCAAAAAGAATCTTT6740.10706791187389496No Hit
GTGATCCCTTTGCCCCAGACGTCCATGTAGTAGGCGTAGTCCCATACATC6730.10690905740523934No Hit
GTGGTCCCTTTGCCCCAGACGTCCATCCCCCTCAGGAATCTCGCACAGTA6720.10675020293658372No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAGGCCGAGTGAGGTCGATCTCTTAC6690.10627363953061682No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAAATTTTAACAGCTGGTACTACGTG6680.10611478506196118No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCCGTAGTACCAGCCAC6680.10611478506196118No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCGACCAGCCA6680.10611478506196118No Hit
AGGATTCCCTGGCCCCAGTAGTCAAAATACCCCCAACTGCCAGCTGCTGT6670.10595593059330556No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCATCGTA6670.10595593059330556No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCGCAACTGGTACACCCCTGC6660.10579707612464993No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATCCGTACCAGCTGCTGCGCGGATC6640.10547936718733866No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACGGCGTACAGTAATA6640.10547936718733866No Hit
AGGGTTCCCTGGCCCCAGTACTCCAACGGGGCCACTCCAAAAATCGTACC6640.10547936718733866No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCGGCTGTTACCACCGTGCCCCC6620.10516165825002739No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTGGGTACCAGCCACTGCCGT6600.10484394931271615No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAATAACCACTACTATCTCTCGC6590.10468509484406051No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGAATAGATGTCTCGCACAGTA6590.10468509484406051No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCCCTTGTGCC6580.10452624037540488No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATAAGTAGC6560.10420853143809361No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACGACGCGCTACTATACGG6550.104049676969438No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGAAGTGGTACGTCCCCAGCCG6520.1035731135634711No Hit
ATGGTCCCTTGGCCCCAGATATCAAAAGCATGCCAACTGTCGCTACCCTT6500.10325540462615983No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCTAACTCCCCGAACCATCC6480.10293769568884859No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTCGAACGGTAT6450.10246113228288167No Hit
TGGGTTCCCTGGCCCCAGTAGTCTCTCCCGACAACAACTCCCCGACCCAC6450.10246113228288167No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAAATAACATATAACTCCCC6430.10214342334557043No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCGTTTTTT6420.1019845688769148No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATTGGACCA6410.10182571440825916No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATAGGGA6390.10150800547094789No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCAGCCTTTGT6390.10150800547094789No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTATTAACCACTACTATCAT6380.10134915100229226No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCCTTGGTAT6380.10134915100229226No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCTACAAT6370.10119029653363665No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGGGA6360.10103144206498102No Hit
AGGGTTCCCTGGCCCCAGGCCCTATCATAGTATCCACCCCATAGCAAGGG6330.10055487865901411No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGCGTCGATAATAACCAGT6320.1003960241903585No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCGAGCCCCGTCCTCCCGC6300.10007831525304722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)