FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011060
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC86050.8510869780230648No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA25350.2507269598243428No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG19060.18851502383636976No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC16160.15983225525685915No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGAAGTGGCAGCTACCA15520.1535022649496568No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAATA14760.145985401459854No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATTCGTAATTATA14280.14123790872945227No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGGC13980.13827072577295116No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG13770.1361936977034004No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCGCTTTTGGCAGCT13570.13421557573239964No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCT13250.13105058057879848No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTGAGAATCACTTCTATCATA13210.13065495618459835No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAGGGCGTAGTCCGAATCTCTCGCACA13120.129764801297648No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATGGTAGACTAATACAATATCTCT13090.1294680830019979No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGTCTCGCAGGGCTCGCG13010.1286768342135976No Hit
GTGGTCCCTTTGCCCCAGACCTCCATGTAGAAGTACTAGTCCTCATAAGT12990.12847902201649752No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCATGATGCTATGGG12900.1275888671295472No Hit
AGGGTCCCCTGGCCCCAGTAGTTGATGTGGTACCAGTTGTTGCTGGTGTA12900.1275888671295472No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCCTCCGATGCCATGTCT12830.12689652443969696No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAACGGGTAGT12820.12679761834114692No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCACGCATCATAGTAAGATAA12620.12481949637014617No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACGGAAACCC12570.124324965877396No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATTGCTTTGG12530.12392934148319586No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAAGTTC12530.12392934148319586No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTGACTATTGGCGTCTCTCACACA12400.12264356220204536No Hit
AGGGTTCCCTGGCCCCATCCGTAGTCACTGCTCGCACAGTAATACACGGC12390.12254465610349534No Hit
AGGGTTCCCTGGCCCCAGTAGTCGGAGTGACCGCCGTAGATGCGCCTCGT12370.12234684390639526No Hit
ATGGTTCCCTGGCCCCAGTAGCCAACTGGCACTGCTAAACCGGGTCTTAA12290.12155559511799498No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGGGCATA12270.12135778292089491No Hit
AGGGTTCCCTGGCCCCAGTAGACTAAAACCAAGGACGAGCTGCTATGGGT12200.12066544023104464No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGGAGGGCCCTCGCACA12170.12036872193539454No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCAGACTCGCTATA12150.12017090973829446No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCCGCGCTTGAGCTGTATCCTTCTCT12110.11977528534409432No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGAGTTCCCCACGAC12080.11947856704844421No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCAAGAGCAGCTACCACCTC12060.11928075485134414No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCCGT12050.11918184875279411No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGATAATCATGATAACCAGTCCT11970.1183905999643938No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATTCATAAGTAGT11960.11829169386584376No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATTGCACCTTCGGACCAGTCAAAATA11940.1180938816687437No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAAGAGGGGGAATA11860.11730263288034341No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCGTCGA11850.11720372678179337No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTCGTCACCAAAATCGCACA11800.11670919628904318No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCCGAAGGTTGTATAGCATA11750.116214665796293No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGATAACC11740.11611575969774296No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGGAGTTGTAAGAATACTC11720.1159179475006429No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCGAGATAATAACC11700.11572013530354282No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCGTAACCATAGCTGTATCC11680.11552232310644275No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCTAAGGGATACCAGCCACT11650.11522560481079264No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACTCGGATCTCACTCCAAA11640.11512669871224258No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCATTTCG11600.11473107431804244No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCCCCATCATAGCAGCT11590.1146321682194924No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGGGCGCTCCAAAAATCGTAATTAG11540.11413763772674224No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGCCACTCCAAAAATC11530.1140387316281922No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCAGCCACGGTGAGTCAGCC11460.11334638893834195No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTACTGGAATCCCAGTGCCCG11440.11314857674124186No Hit
AGGGTTCCCTGGCCCCAGTACCGGTAGTCACCGTAGTCTGGTGCAGCTCT11330.11206060965719146No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTGGACCA11320.11196170355864143No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGAGTGGGTAGCAGCTACCACC11240.11117045477024112No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCAACTCTCGCACAGTAATACAC11240.11117045477024112No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG11200.11077483037604098No Hit
AGGGTTCCCAGGCCCCAGGGGTCGAACCAGTCACGTCCTCTTGCACAGTA11170.11047811208039088No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTAGTCCCCCAGCCACTGCT11110.10988467548909066No Hit
GTGGTCCCTTGGCCCCAGAGGTCCATACCGTAGTAGGATAGGTAGGTCCC11110.10988467548909066No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATTGGGT11090.1096868632919906No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCACCGTTCCC11090.1096868632919906No Hit
AGGGTTCCCTGGCCCCAGTGTTCAAAGTTAATACCAGCCACTGCCACCCA11050.10929123889779042No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCTTTCCCCATACCGTATAA11020.10899452060214032No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATTGGTAATAACCACTACTATCATA10970.10849999010939014No Hit
GTGGTCCCCTTGCCCCAGACGTCCATGTAGTAGTATGAGCCTACTAATCC10960.10840108401084012No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCACGAAAATGGCAGCAGC10950.10830217791229008No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTTAGCGGGTGCCCCGCC10940.10820327181374004No Hit
ACGGTGCCCTGGCCCCAGTGCTGGAAGTATCTAGTGCAGCTGGTTGGACT10940.10820327181374004No Hit
GTGGTCCCTTGGCCCCAGACGTCCTCTGGGTTAATAACCACTCCAAAAAT10940.10820327181374004No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCTAGGGACGA10910.10790655351808993No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGACTATAACCAGTCAAAA10890.10770874132098986No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTATGAGGAGCAGCTGGT10810.10691749253258956No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGAGCCACT10740.1062251498427393No Hit
GTGGTCCCTTGGCCCCAGACGTCCATCGATGTGGAATAACCACTCCAGGG10740.1062251498427393No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAAGGCCACCCT10740.1062251498427393No Hit
AAGGTTCCCTGGCCCCAGAAGTCAATATGCGGGTTAGTAGCTCCCATTTC10740.1062251498427393No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGGGTGAGTAGTCACCGTATAGGTC10700.10582952544853916No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAAGTCGAGCTGCT10690.10573061934998913No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGTCATC10670.10553280715288905No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTCCTCCCCTCGTTCCAGT10660.105433901054339No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAGTAACCCGAAATTATCACTCCCTGA10620.10503827666013886No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTATAACCATC10550.10434593397028862No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATAATAGTAGGGGTAGCAGTC10510.10395030957608847No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGCATGAACCATAGCTGGATCCCGA10500.10385140347753843No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCACCTAT10490.1037524973789884No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGAGATG10470.10355468518188833No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGGACCCGCGTA10460.10345577908333826No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTCCGAGCTGCTGAGACTCT10420.10306015468913812No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGGTGCTACCA10410.1029612485905881No Hit
AGGGTTCCCTGGCCCCAGAAGTCAAAATCGTATGCCAGCCAGCGACAGAA10390.10276343639348802No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATTCCGAACC10370.10256562419638796No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGACGTA10370.10256562419638796No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCAAGTGGTAGCAGCTA10370.10256562419638796No Hit
GTGGTCCCTTGGCCCCAGACGTCAGTTCCACCCGGAACTCTCGCACAGTA10370.10256562419638796No Hit
AGGGTTCCCTGGCCCCACTCCACTGAACCCGGACAGAGCCGTACCAGCTG10360.10246671809783792No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAAGA10280.10167546930943763No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCGTCCACCCAA10260.10147765711233754No Hit
AGGGTTCCCTGGCCCCAGTAGACCAGGGCGGGATAATAACCAGTCAAAAT10260.10147765711233754No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGATCGTTCCAGTTATAAT10240.10127984491523746No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGACGATCACCGTAGCAGCTACCACCA10220.1010820327181374No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCAGCTCCCACTATAGA10220.1010820327181374No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGAGTAGTAGTAATATTG10210.10098312661958736No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGCGATCCCAAA10200.10088422052103732No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTGGACC10180.10068640832393726No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAAGGGGAAAGATAGCA10140.10029078392973712No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGTCATGGTTTCGGGCAACTGG10130.10019187783118708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)