FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006287
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC82830.8231250130429987No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC41180.40922718866486396No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA23960.23810304614886207No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCACGGCA15450.15353472717028044No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC15140.15045409510408064No Hit
AGGGTTCCCTGGCCCCAGGAGTCGAACCAGTCTGTCCCAGCTGCTGCTAT14620.14528658325110033No Hit
AGCTCTCTCCACCCGTCGGTAACTCCCCGAAGCATAATGAAACTGCACAT14540.1444915814275649No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGGGA14390.14300095300843596No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCGAATAATA14140.14051657230988773No Hit
AGGGTTCCCTGGCCCCAGGGGTCCACTACAATATCCAATTCCCCTCTCGC14030.13942344480252652No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCCCCGGGTATAATAACCAC13470.1338584320377785No Hit
AGGGTTCCCTGGCCCCAGTAGTCATCATAATAACCACTACTATCATAGTA13360.13276530453041727No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTCGGTTG13290.13206967793482374No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCGGGTAGT13290.13206967793482374No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCTCCCCGAAC13250.13167217702305603No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCAAGGT13160.13077779997157868No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCACGGGCAGCTGGTACCCA13120.13038029905981097No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGACCATAGC13100.1301815486039271No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAACTCCCCGAAAC13070.12988342292010133No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGAAAATATAATAACCAGTCAA13050.12968467246421744No Hit
AAGGTTCCCTGGCCCCAGTAGTCAAAAGCCCAGTTGTGATCTCTCGCACA12550.12471591106712102No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTATGAGAGATAACC12550.12471591106712102No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACGACCACTCCAAAAATCG12430.12352340833181785No Hit
ATTGTCCCTTGGCCCCAGATATCGAAAGCACCTTTCGAGCTGGTATAGTC12430.12352340833181785No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATAACCATAGCTGTA12410.12332465787593401No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAGGTGCCGAGGTTCAGTCGCACAGTA12350.12272840650828243No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAGGAGG12340.1226290312803405No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTGGGGGAA12300.12223153036857279No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAAATCACC12290.12213215514063085No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGATGTGTATAATAACC12270.121933404684747No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCGAGTAGCA12210.12133715331709542No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGTAGTAGTAGTCTGCTACAGA12180.12103902763326962No Hit
AGGGTTCCCTGGCCCCAGTAGTCATAGGGATAATAACCACTCCAAAAATC12130.12054215149356No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGCAGCTG12020.11944902398619878No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATTCCCCAGCCACTGCTCCTC12020.11944902398619878No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTGGCCTCTCAAATGTTGTTC12010.11934964875825683No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTGGGATAC11970.11895214784648912No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGTGGAGTAGTCACCGTA11950.11875339739060525No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGAGACCGCAGCCACTGCTAATAGAGT11940.11865402216266333No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAAGCCA11940.11865402216266333No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG11920.11845527170677948No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGGGCTGTAGCCTCACCGAGGGT11900.11825652125089561No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCCTCCGGC11870.11795839556706983No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTAGTAGGGGGATAGCATACG11820.11746151942736019No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGCCCACTGC11810.11736214419941825No Hit
AGGGTTCCCTGGCCCCAGAATCCCCGCACGAGCTGCTATACTCTTTCGCA11800.11726276897147632No Hit
AGGGTTCCCTGGCCCCAGTAATCGGGCGAGCTGCCCCCTTTTCCATGGTC11790.1171633937435344No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATTGCGGGAGGTATTGGGC11790.1171633937435344No Hit
GTGGTCCCTCTGCCCCAGACGTCCATGTAGTAGTAGGAAATAACCACTCC11790.1171633937435344No Hit
ATTGTCCCTGGGCCCCAGAGATCAAAAGCCCACCATACATCTCTCACACA11780.11706401851559248No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCATTTCCTCTAGTAGC11770.11696464328765054No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATCCCCGGGGGAT11760.11686526805970861No Hit
AGGGTGCCCTGGCCCCAGTAGATGAAGTAGCAGGCGCTACTACTACAATA11760.11686526805970861No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCCGAGTAATAACC11730.11656714237588282No Hit
AGGGTTCCCCGGCCCCAGTGGTCATGGCATTATTAATAACCATTATTCTT11710.11636839191999898No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGTCCCTCTCCCAAAACGT11690.11616964146411511No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTCGTAGCGTAGTCACC11640.11567276532440546No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGGA11630.11557339009646353No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAAATTGTGGGTAACCACTC11630.11557339009646353No Hit
AGGGTTCCCAGGCCCCAGTAGTCAAAGTTGGGGGTGGCTGGCTCTCTCGC11610.11537463964057969No Hit
AGGGTTCCCTGGCCCCAGCCCCATAAACCAAACCCGGTAGGTTGAGTCAC11610.11537463964057969No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCAGCCACTGCTTATACGGCATACACC11590.11517588918469582No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAAGGGA11580.11507651395675389No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTTTGACCCCCG11580.11507651395675389No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCTTGGGC11580.11507651395675389No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTTGGGGC11550.1147783882729281No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGCTAGACGAGCTCGATAGTAATAACC11550.1147783882729281No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCAATCGATATA11500.11428151213321845No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAACCCTCACC11390.11318838462585723No Hit
AGGGTTCCCTGGCCCCAATAGTCAAAGTCTTTCCTCGGCCTAGGCCCCTC11390.11318838462585723No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCCCCTTACCAGCTGCTG11380.1130890093979153No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTGTTTTGGGCCGCCAAAT11370.11298963416997339No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTTCCCCCA11340.11269150848614759No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGCAGCCAGCCACTGGCCACTATCTTT11330.11259213325820565No Hit
AGGGTTCCCTGGCCCCAGTAGTCATTTGCAGCTGGTACCGCTCTCGCACA11330.11259213325820565No Hit
AGGGTTCCCTGGCCCCAGTAGACCAAATAGCAATCACCACCACAATACCC11170.1110021296111348No Hit
AGGGTTCCCCGGCCCCAGAAGTCAAAAATGTCATAACTGTATGCACTCCC11150.11080337915525093No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCGATGTAGCAGCTACCAC11140.110704003927309No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATCGTTTTTG11130.11060462869936709No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCTCCTCGTGGTAGCTCT11110.11040587824348322No Hit
AGGGTTCCCAGGCCCCAGTAGTCAAGGGGGTGACCAGTCAAAATATCATA11090.11020712778759935No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATTTGATACTGTA11080.11010775255965743No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGGGGTAAATAACTCCCC11050.10980962687583164No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACTGGAGATAACCAGTCAAAATATC11030.1096108764199478No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCATCCAA10970.10901462505229621No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAATAATGCCACTCCAAAAA10960.10891524982435428No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCCCGGGAGGGTAGCGGAGGT10950.10881587459641234No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATCCCAAAATAACTCCCCG10890.10821962322876079No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGAACGTAGTCACCGTAGTCCTT10890.10821962322876079No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAACCGTATCGAAATCC10870.10802087277287692No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATTA10860.10792149754493499No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCCCATCGTGTA10860.10792149754493499No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCTCCATCATGGCTCCCTGAACC10790.1072258709493415No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGGCTCTCTGTGTGGTTCCAG10790.1072258709493415No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGATGTCCCC10760.10692774526551571No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTGCCCCCTCCCCGAACCATA10760.10692774526551571No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCCCCGGTGTCCGTTCTGGA10740.10672899480963184No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAAATCGGAAACCTGGGTCTCTCGC10740.10672899480963184No Hit
AGGGTTCCCTGGCCCCAGAGCCCATGGTATTGCGTGGAACAGTAATACAC10720.106530244353748No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCAGGACGGTA10700.10633149389786413No Hit
GTGGTCCCTTGGCCCCAGACGTCCTCCCCACGTTCCAGAAGGGACCCGCC10690.1062321186699222No Hit
AGGGTTCCCTGGCCCCAGTAGCCCCGGTATAGCAGCTGGTACTACTACAA10680.10613274344198027No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTGCTCCACCCCCTTGCGTCC10650.10583461775815449No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCCGCGACAGAGACTCT10620.1055364920743287No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGGGAGGTAAGTGTAACCAGTAGT10600.10533774161844484No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCAAAGTGAGGGATAAGGGCCAC10580.10513899116256098No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGTTGAACTGGTATAAGTTGC10570.10503961593461904No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGATATAGGGTAATAACCACT10560.10494024070667712No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAATAACCGTCATC10530.10464211502285133No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAGTAGTTACT10520.10454273979490941No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGAGGCAGCTGGTACT10500.10434398933902554No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCACTTACGTACCAGCCACTGCTCGA10490.10424461411108361No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTGAATATG10470.10404586365519974No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCGGTTGGG10470.10404586365519974No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATCTTGCTGCCCTCAACTC10470.10404586365519974No Hit
AGGGTTCCCTGGCCCCAGTAGTCATGGTAATAACCACTCCAAAAATCGTA10470.10404586365519974No Hit
GTGGTCCCTTGGCCCCAGACGTCCACCACAGCCACTGCTATACCCGGGTC10390.10325086183166432No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCGCCAAATTAAATCTCTCGCACA10380.1031514866037224No Hit
AGGGTTCCCTGGCCCCAGGGGTTGTACCTCATACCTCCCCTCGCACAGTG10380.1031514866037224No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCCTCGGAGTAATACCCC10370.10305211137578048No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATTGGTCGGGGGA10350.10285336091989661No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCTTCGAGTCCTGGGATACC10320.10255523523607082No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCGGGACGGTCAAAATAGC10310.1024558600081289No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGGGGACGTA10310.1024558600081289No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGACGCTACTATCATAGTA10300.10235648478018697No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAATGGTGGCAGCTGGTACGA10300.10235648478018697No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTACCTCCCCAG10270.10205835909636118No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATTTGTTGTAGGGGTAATAACCGAG10230.10166085818459344No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGGGGTAATAACTCCCTGAACC10220.10156148295665153No Hit
AGGGTTCCCTGGCCCCAGTAGCCCGCTACCAGCCACTGGTCCGTGGTCCG10200.10136273250076769No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGGGTACTTATAGAAGGGGGCCAT10190.10126335727282573No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCAGCTACCACCACTACT10190.10126335727282573No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGCTGCAGTCGG10160.10096523158899995No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCC10140.1007664811331161No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAAGG10120.10056773067723224No Hit
GTGGTCCCTCTGCCCCAGACGTCCATGTAGTAGTAGTAGTAATAATATGC10100.1003689802213484No Hit
AGGGTTCCCTGGCCCCACTCCACCAACGAGCTGCTGGGAGTTTGGGCCGC10100.1003689802213484No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTATGAGAA10090.10026960499340644No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTTCGTGC10070.1000708545375226No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGGGTAACTCCCTGAACC10070.1000708545375226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)