FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001132574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787177
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC109161.3867275085527142No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA30260.3844116380432863No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC23400.2972647828887277No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG16710.21227754367823246No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGGAA14280.18140773930132614No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCCTCAATTGCCACTGGAT13540.17200705813305012No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACTCAGCCGTAGACCC12340.15676271029260255No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGACTGCAAGTAGCTCCC12200.15498420304455035No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGAGTTAAGTACGTAGTT12110.15384087695651677No Hit
AGGGTTCCCTGGCCCCAGTAGTCGGCGGAGTTACCACCGTAGGACACTGT11500.14609166680428926No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTTAGTACAAC11500.14609166680428926No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAATA11270.14316983346820347No Hit
ATTGTCCCTTGGCCCCAGAGGGGGTTGGCTGTCAAAATATCGTAATATGT11100.14101021752414006No Hit
AGGGTTCCCTGGCCCCAGTAGCTCCCGCGATCTCTCGCACAGTAATACAC10810.1373261667960319No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTATCTCGTGGAGGTAGC10770.13681802186801698No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTTCTTCCAGCCACTCCCC10650.1352935870839722No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGGATAGTAGCTCCCACT10520.13364211606792373No Hit
AGGCTTCCCCGGCCCCAGTGGTCAAAGAAGTAGAAGTTGACATCACAAAA10490.13326100737191254No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGACTCCC10340.1313554638918566No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGAGGTCTCTCTTGCACA10320.13110139142784916No Hit
AGGGTTCCCTGGCCCCAGTAGTCACTAAGGTATCGTCCCGCTCTCGCACA10310.1309743551958454No Hit
GTGGTCCCTTGGCCCCAGACGTCCATCCCGTAGTAGTAGGCGAAGTCCGG10300.1308473189638417No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCACCAGC10210.1297039928758081No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGAACCGAGTAGCTCCCACCCAAG10200.1295769566438044No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGACTTTA10200.1295769566438044No Hit
AGGGTTTCCTGGCCCCAGGGGTCGAACCACTTTTGGCCCTTAGTACACAG10180.12932288417979693No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTCAAATA10060.12779844939575216No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGTAGTCGGCGGAAGAGAGTCT10010.1271632682357335No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTACCAGGACGAGCT9990.12690919577172605No Hit
ACAGACAGGCCACCCAGGGCTCAGGCTGGGTATGGTGGGCTCTGGTGGGA9990.12690919577172605No Hit
GTGGTCCCTTGGCCCCAGACGTCCACAAGTCCCACGAACCTGAAAAATGT9950.12640105084371112No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGTCATAATAACCACTCCA9930.12614697837970368No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGCTCCCCGATCTTTCGC9790.12436847113165145No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGTTCCCC9780.12424143489964772No Hit
AGGGTTCCCCGGCCCCAGTGGTCTTGTTTACTCTGCGAAGTATAAATGTT9780.12424143489964772No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGAGGGAACCACTCCAAAA9760.12398736243564028No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTGGATCCAGCCACT9720.12347921750762536No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGTC9690.12309810881161416No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACCCACTAACCATAGCTGT9670.12284403634760671No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCTAGTGC9670.12284403634760671No Hit
AGGGTTCCCTGGCCCCATGGGAAAAAAATTAAAGCCACCTCTGTCGCACA9660.12271700011560298No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACGTAGTCACCGAGAACTCTCGCACA9610.12208181895558433No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAAAGCCTTC9600.1219547827235806No Hit
AGGGTTCCCTGGCCCCAGTAGCCCGAGGGGTAATAACCACTACTATCATA9580.12170071025957313No Hit
AGGGTTCCCTGGCCCCAGTAGTCATGTTGCGCCAGCTGCTGCTATCTCTC9540.12119256533155821No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCACTAGGGG9520.12093849286755076No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACACTCGTCGTAGCCATCTCT9500.1206844204035433No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCGCGGGGTCGGTATATCACTACTATC9490.12055738417153956No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCAGTAGTAGTAGTACCTGAC9470.12030331170753211No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTGAAGCTGTATCCACGTCCC9460.12017627547552838No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACCCCAC9460.12017627547552838No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCGTAGTAGC9460.12017627547552838No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGGGAGTTACCACCGTAGCGGAC9440.11992220301152091No Hit
GTGGTCCCTTTGCCCCAGACGTCCATCCGGGACCCACTGCTCAGATCTTT9440.11992220301152091No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTTAGCAG9420.11966813054751346No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGGA9390.11928702185150226No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAATGGGT9370.1190329493874948No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAATAGCAGCTGGTACT9370.1190329493874948No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAAGGGGACCTGAACATAGTAGGGCC9340.11865184069148361No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCTCAGTGCCTTGGACTCCCT9330.11852480445947988No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGGGCTCGATTT9310.11827073199547243No Hit
GTGGTCCCTTGGCCCCAGACGGCCAGACCATACCAGAAGTGGATATCTCG9310.11827073199547243No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCCATTCCCCA9250.11750851460345005No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAAGGGTTCGGAAGCGGCAA9160.11636518851541647No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCCATTCCATAGTAACGAC9150.11623815228341275No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCGACTCGAGAACTACCAGCCA9130.11598407981940528No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACTAACGCTTGTAGCCACCACGAC9120.11585704358740155No Hit
AGGGTTCCCTGGCCCCAGTAGCCCGGGGGCTCCCACTAGCCCTATAGCAC9110.11573000735539782No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAACTCCCAAAGGGTATAGCTCCCACT9110.11573000735539782No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCAAAATT9090.11547593489139038No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGCGCCCGTA9070.11522186242738293No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCAGCTT9050.11496778996337546No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGAAGTCGGACTTTCC9040.11484075373137173No Hit
GTGGTCCCTTGGCCCCAGACCAACGAAGTAGTTCCACCCCTCTCGCACAG9040.11484075373137173No Hit
AGGGTTCCCTGGCCCCAGTACCATTGGATATAGCAGCTGGTACTACTACA9040.11484075373137173No Hit
ATTGTCCCTTGGCCCCAGATATCAAAGGTACCAGCCACTCCAGCTAACGG9020.11458668126736428No Hit
AGGGTGCCCTGGCCCCAGTAGTCAAAGTAACCCGGGACTCTCGCACAGTA9010.11445964503536055No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAAACCGCATCAGGGGTAACCACTCC9000.11433260880335681No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTTCAGTAAGGA9000.11433260880335681No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAGTTACC8990.11420557257135308No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACCAAACAGCCACCGCCCCTTC8960.1138244638753419No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCGACAT8930.1134433551793307No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAATAACCACT8910.11318928271532325No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGCCGAGGCCGGGAG8880.11280817401931205No Hit
ATTGTCCCTTGGCCCCAGATGGGAAAGTCACCACTTTCAAGTTTGTCTTC8870.11268113778730832No Hit
GTGGTCCCTTGGCCCCAGACGTCCGCACCCCCCGCAGTTACGTCCTTAAC8850.11242706532330086No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGTGGCTG8820.11204595662728967No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAGTAGCA8790.11166484793127848No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATTCCCCCCAAATGTAATCATAAA8770.11141077546727102No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCCTGAGGGCCTACGACGACC8750.11115670300326357No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCAATAGC8690.11039448561124118No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTTCTCACCCCGAGTAGC8640.10975930445122253No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACTCATCTCTCGC8640.10975930445122253No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAATTAGG8630.1096322682192188No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGAGCAGTAACCAGTCAAAATATCGTA8630.1096322682192188No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCAAATC8620.10950523198721507No Hit
AGGGTTCCCTGGCCCCAGTAGGCCAGAATTGTAGCCATCCCCTCCCCCTC8600.1092511595232076No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACGGCACCGTAGTCGGTAGGTCTCGC8560.10874301459519269No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATTTTCG8550.10861597836318895No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTAGT8540.10848894213118523No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGGTCGCTAGCTGCTGC8520.10823486966717777No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACCAGCCGGGGGTTAC8510.10810783343517404No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGGCTCTGGA8490.10785376097116658No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGACAATAACTCC8450.10734561604315165No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACGAGTGTTCGGGGGGTGCTCGGGA8440.10721857981114792No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGATAGCAGTCACCACCCCAGC8420.10696450734714048No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGTAGTCACCGTAGGGGCTCTCGC8410.10683747111513676No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATAGTAGTAGTAAACAGAACC8410.10683747111513676No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACCTGG8410.10683747111513676No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACGGGCTGTTACCACCGTA8380.10645636241912557No Hit
AGGGTTCCCTGGCCCCAGTAGTCGCCCAAGTAGGTCCCACTATCTCTAGC8370.10632932618712183No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGCCCCGGGTAT8370.10632932618712183No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCACCCAGTCAAAATATC8370.10632932618712183No Hit
GCGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTCCAATAC8360.1062022899551181No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAAAGGGTT8350.10607525372311438No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACTTAAGTTACTCTCTTGTCGCGGGT8340.10594821749111065No Hit
GTGGTCCCCTGGCCCCAGACGTCTAAACCGTAGTAGGTCAGGCCACTGAG8330.10582118125910692No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGAAG8320.10569414502710318No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATAT8310.10556710879509945No Hit
AGGGTTCCCTGGCCCCAGTAGTCATTATGGGGCGATAAGTCCCCCAAACG8290.105313036331092No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGGACCAGC8260.1049319276350808No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAACGCCAGTCACC8240.10467785517107335No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAGATTACTGTA8230.10455081893906962No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACGTAGC8220.10442378270706588No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTCCTTACAAGGAGTAGCAG8210.10429674647506215No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCTCGAACCCGGCCCGCTACTGCC8210.10429674647506215No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGAGATAATAACTCCCTG8200.10416971024305843No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACATCATAGC8190.1040426740110547No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAAAAACATAGC8190.1040426740110547No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCTGCAGC8190.1040426740110547No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCAACATAAAAAGAGTAGCAGCTACCA8180.10391563777905097No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAATAACC8160.1036615653150435No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGGATAGCAGC8160.1036615653150435No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGACCCACTAC8160.1036615653150435No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGTA8150.10353452908303977No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATGGGCCTACCGTAGTAAT8130.10328045661903232No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTTAAGGA8120.10315342038702859No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCACTACCAGGCCCGAACCATA8110.10302638415502485No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTATTGGGGGTA8080.10264527545901367No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATGAGTTGTGATGGAGACGATAACT8070.10251823922700994No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA8060.1023912029950062No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG8050.10226416676300247No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGCCTTAT8020.10188305806699129No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGACCTCCCT8020.10188305806699129No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATACGCGATAATAACCAGT7980.10137491313897637No Hit
AGGGTGCCCTGGCCCCAGCGCTGGAGCGCATTCCCAACGCGATAGTAATG7960.1011208406749689No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTTCCCTAGAGC7950.10099380444296517No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCACC7890.10023158705094279No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGACGA7880.10010455081893907No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAATAGGGTG7880.10010455081893907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)