FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001132576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414999
Sequences flagged as poor quality0
Sequence length167
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCTCACTTCCCGATCCCACTT89122.147475054156757No Hit
GTGGTCCCTTGGCCCCAGACGTCCACCCAGGGCGGTAGCATACACCATTA52351.2614488227682477No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATAG28130.6778329586336352No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCGGCCACCCCCCG27430.6609654481095135No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAATACCCCCCTCTCTCTCGCAC25040.6033749478914407No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCGGACGAGC18240.4395191313714009No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATAACTCCCCGAACC18150.4373504514468709No Hit
GTGGTCCCTTGGCCCCAGACGTCGCCTCTCGCACAGTAATACACAGCCGT12230.29469950530001277No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGGCCCC7700.1855426157653392No Hit
GTGGTCCCTTGGCCCCAGACGTCCACCCAGGGCGGTAGCGGGGTACAGTA5940.14313287501897595No Hit
AGGGTTCCCTGGCCCCAGTAGTCTCCTCTAACTTTATCCCCAGCCCCAAT5130.1236147556982065No Hit
GTGGTCCCTTGGCCCCAGACGTCCACCCAGGGCGGTAGCATTCCCCGCCG4870.11734968036067556No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTACTA4620.11132556945920352No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC4220.10168699201684823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)