FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808966
Sequences flagged as poor quality0
Sequence length167
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC85081.0517129273665393No Hit
ACAGACAGGCCACCCAGGGCTCAGGCTGGGTATGGTGGGCTCTGGTGGGA20140.2489597832294559No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG17060.21088648966705648No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG16510.20408768724519943No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC15980.19753611400231902No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGTAG15970.19741249941283068No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTCAG15230.18826501979069576No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAACCATAGCT14990.18529826964297635No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA14900.18418573833758156No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAAGA12900.1594628204399196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAAAC12290.1519223304811327No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAATA11960.14784304902801848No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCGTTCCAGTTATACCCAA10980.13572881925816413No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCCACCGAGGGTCTAAGATAACC10230.12645772504654088No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATAGTGG10190.12596326668858765No Hit
GTGGCCCCTAGGCCCCAGACGTCCACATCATAGTAGTAGAACTAACCCGA10180.12583965209909934No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACTGTAGTAGTCGTGGTACCAGCT10160.12559242292012274No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGTACGGAAGTACTCCTACGGC10060.12435627702523963No Hit
GTGGTCCCTTGACCCCAGACGTCCATAGCGTAGTAGTAGTAACCATAGCT9830.1215131414670085No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGAGTCCCCCCTCACC9810.12126591228803188No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACCGTAGCAGCTGG9790.12101868310905525No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCCGAGAAAGAGGATGT9770.12077145393007864No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCAGCTGGTACTACTACAATA9720.1201533809826371No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCTAT9700.11990615180366047No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCCGGGC9590.11854639131928908No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCGATCACAGCTCCCACTAA9580.11842277672980075No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAAGGATTAGAGCC9540.11792831837184753No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCAACCAGTCAAAATATCCGG9480.11718663083491766No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTCTCTC9360.11570325576105793No Hit
TGGGGACAGGGACAGGTGGTCCTGGGATCAGGCTTCAGCTGCACGTCCTG9330.11533241199259302No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTGTTCCGGGTATCGAGT9270.11459072445566315No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTTTTTACAGA9270.11459072445566315No Hit
GTGGTCCCTTGGCCCCAGACATCCATACCGCGCGGGGCTATCACACCTCC9210.11384903691873328No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGGATAGTACCAGCTGC9200.113725422329245No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGCAGTAACCACTCCAAA9170.11335457856078004No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCGGCGTTGTAGCCATCTGAGGT9120.1127365056133385No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCAGTTATAGC8970.11088228677101385No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGAGCAGC8950.11063505759203725No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCCCATTCCAGC8950.11063505759203725No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCACCGGC8880.10976975546561907No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATAAACC8850.10939891169715414No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCATAATAGCCGTGATCTCTCGC8850.10939891169715414No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA8820.10902806792868923No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAAATCC8780.10853360957073596No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACGAGCTGCTGCTATACGG8770.10840999498124768No Hit
GTGGTCCCTTGGCCCCAGACGGCCATACCGTAGTACCTGGGGGCCCATTT8750.10816276580227105No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCCCTTTTATAACCACT8740.10803915121278274No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATAGTAG8740.10803915121278274No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTGTATAGCAGCTGG8720.10779192203380612No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCAGATTATA8710.10766830744431781No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATACACTCCCCGA8710.10766830744431781No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCAAAATAACCACT8670.10717384908636457No Hit
AGGGTTCCCTGGCCCCAGTAGTCCAAGTAGTCACCGTAGTCATCGAGGAT8640.10680300531789963No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATTTTCCTAA8630.10667939072841132No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCTGGTAA8590.10618493237045809No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATAAGCCGTAACTCCCTCG8560.10581408860199316No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATGGGCTACCAGCTGCTGC8560.10581408860199316No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACACACTAAGGGCATA8560.10581408860199316No Hit
AGGGTTCCCTGGCCCCAGCCCCCAGCCCCGGATGGAGTTCTCGCACAGTA8560.10581408860199316No Hit
ATTGTCCCTTGGCCCCAGATATCAATAGCTGCTGCTATTCCCGTATCTTT8550.10569047401250485No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCGCCCCCCACCGTTTCTCT8550.10569047401250485No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAACGGG8540.10556685942301654No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAATGATG8510.10519601565455161No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCGAGTACCAGCCACTGCCCG8500.1050724010650633No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGAGGTTGGGTAACCACT8440.10433071352813343No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCATATGTT8430.10420709893864515No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACCGACGAAAACAGGTTAG8420.10408348434915683No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCTAATTGTAGCCATCTCTAC8420.10408348434915683No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAATTTACTGTATTTGTACGG8420.10408348434915683No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGATAACCACTCCAAAAATCGT8410.10395986975966852No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTTTTTAAT8400.1038362551701802No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGAAGGTTG8390.10371264058069189No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGGT8370.10346541140171528No Hit
AGTGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGGCACCGAAATCCACCT8360.10334179681222697No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAGTTACC8220.10161119255939063No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGAGTAGTAAACTCCCCG8210.10148757796990232No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAATAGGCCCAATC8210.10148757796990232No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCGAAGGGGGGCTT8200.10136396338041401No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCGTGCCATAA8200.10136396338041401No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCTGCAATTTACCAGCCAC8190.10124034879092571No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGCGTCAGA8190.10124034879092571No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCAAAATAATATCGAAAGGGCCT8130.10049866125399584No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGGATCC8110.10025143207501921No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGTATGGGTAGTCATC8100.1001278174855309No Hit
AGGGTTCCCTGGCCCCAGTAGTCATAGCTGCGCCCCAAGATATTGTAGTA8090.10000420289604262No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAAGGAATAACCACTCC8090.10000420289604262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)