FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910065
Sequences flagged as poor quality0
Sequence length167
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATAACTCCCCGAACC32917536.170493316411466No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGGCCCC11908013.084779658595815No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTACTA796508.752122101168599No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCAATAACTCCCCGAACC94611.0395960728079863No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC79010.868179745402801No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATAACTCCCCAAACC78730.8651030420904001No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCTTTAATAA69050.7587370132902594No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCTCCCTTTAGTAGCTCCCAC52840.5806178679544868No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCCGTAG48880.5371044925362475No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCAGTAGTAGAGGGCCCC33650.36975380879387737No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGACCCC31180.34261289028805636No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCAGCTGCCATCCTCTTGCACAGTAA30610.33634960140209763No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTACCC29640.3256910220698522No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTACCT18480.2030624186184503No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACCACTA18420.20240312505150732No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC16440.18064643734238764No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTACTT16340.17954761473081593No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA15940.17515232428452912No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCTAATAACTC15120.16614197886964116No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG15060.16548268530269816No Hit
ACAGACAGGCCACCCAGGGCTCAGGCTGGGTATGGTGGGCTCTGGTGGGA14900.16372456912418346No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATAACTCCCCGAATC14430.15856010284979644No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTACTG13980.15361540109772379No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAGGGCCCCGTC13370.1469125831671364No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTTCCCACT12940.1421876459373781No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTAAGC12650.13900106036382015No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA12410.13636388609604808No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCGAGGAAACCACT12160.13361682956711882No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAAGGTGGCTCGAAGCAGCTACCACC12140.1333970650448045No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG12140.1333970650448045No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCAGCTACC11900.1307598907770324No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCGTAGCAGC11900.1307598907770324No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCCTT11870.1304302439935609No Hit
TGGGGACAGGGACAGGTGGTCCTGGGATCAGGCTTCAGCTGCACGTCCTG11870.1304302439935609No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTACCG11750.12911165685967485No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCAGCTGCTGCTATCCCCATCTCTCG11700.128562245553889No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCTGTCGTAACGATCTCTCGC11620.12768318746463164No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGACTATAGTAGTCCTCGC11500.12636460033074562No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCGGCGTAGTCACCGTGGAC11420.12548554224148825No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTAGGGTCT11260.12372742606297353No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGGCGGTCGGGACTCCCCGA11250.12361754380181636No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAATTACTGTAGAG11160.12262860345140182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAACTAA11140.12240883892908748No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCCTAAGCTCCGAGCTGCT11040.12131001631751578No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGTACTACTATCATAGTAAT11000.1208704872728871No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCCTGAACCGT10850.11922225335552956No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCCTTTGATGCCACTCCAA10820.11889260657205804No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATACCCCGCAGCCATGTCC10790.11856295978858652No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCTGTACCA10780.11845307752742935No Hit
AGGGTTCCCTGGCCCCAGTAGTTCTAATCTTTTTATAACGATAACTCCTC10780.11845307752742935No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAGCAGCT10730.11790366622164351No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGCGG10690.11746413717701482No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCGCCCCGGG10610.11658507908775748No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCCGAGGTTTG10590.11636531456544312No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCAGACTCGGGCCTGGA10370.11394790481998539No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTTTATAGC10320.11339849351419953No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTCCTTAGC10300.11317872899188519No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGTAGTAGCTC10270.11284908220841369No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAAAACCATAGCTGTATCC10240.11251943542494218No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCCTGGGTTTAGCAGCTGG10210.11218978864147065No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACACCCC10170.11175025959684198No Hit
AGGGTTCCCTGGCCCCAGTAGTCACAGTCCCACGAGGAACCTCCCACCCT10110.11109096602989897No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACGCCCTAGTTCCAGT10090.11087120150758462No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCGTGGC9970.10955261437369858No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCCACTC9970.10955261437369858No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACTAACAAAGGAG9960.10944273211254141No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACTGGGGGGTTCT9950.10933284985138424No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCGGGCTGGTACTACCGCA9820.10790438045634103No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCTGTTGGGGGGTA9800.10768461593402669No Hit
GTGGTCCCTTGGCCCCAGACGTGGCCCCGCATGATTGTGGTACAGTAATA9770.10735496915055519No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAGCTGGTACTGCAC9750.10713520462824085No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGGATAGCAACTTATCCGTGCA9690.10647591106129782No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGGAGGGCTTCCTCC9690.10647591106129782No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAAGGTC9690.10647591106129782No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCTTTTTAC9550.10493755940509744No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACAACGTCGACGCAGAACCACTACAA9490.1042782658381544No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACGCTTGGGAGCTCCCACTATACG9470.10405850131584007No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCT9390.10317944322658272No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGAAATAACCAGTCAAAA9300.10219050287616818No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCATAACTCCCCGAACCATA9270.10186085609269668No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACCCGTACCAGCCACT9260.10175097383153951No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGCGAGTAT9240.10153120930922516No Hit
GTGGTCCCTTGGCCCCAGACGTCTGTCGGGAGCTCCAAAAATCGTAATAC9210.10120156252575366No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGGGGGTCGGCCACTACT9190.10098179800343932No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCGGTTTC9160.1006521512199678No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAACGTAGTAGCCCCCCTGGGGGAC9140.10043238669765348No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTAGACCTGGTAACCACTACTATC9130.1003225044364963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)