FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236
Sequences flagged as poor quality0
Sequence length208
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCAT8536.016949152542374No Hit
TGTCGCCTGCACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGC3514.83050847457627No Hit
CGGGTCACCTTCTGAATTTTTACCTTCATAAACAGCAAGGACTGCGCTCT2611.016949152542372No Hit
GCCCTGTAATCCCAGCTCCCCGGGAGGCTGAGGTCGGCGGATCGTGGGTG208.47457627118644No Hit
GCCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAA41.694915254237288No Hit
GCCCTGTAATCCCAGCTACTGGGAGGCTGAGGCAGGAGAATCGCTTGAAC31.2711864406779663No Hit
CGAGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCATT20.847457627118644No Hit
CGGGTCACCTTCTGACTTTTTACCTTCATAAACAGCAAGGACTGCGCTCT20.847457627118644No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCAGGTA20.847457627118644No Hit
CGATGTATCCAAGTCTGACAGCCCCAGAAACGGGTGTGCAGGGCGCCCAT20.847457627118644No Hit
AGGGCAGTCATTTGACCACTCCAAAAATCGTAATACCGTCGACCTCTCGC10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTTAGGAGGCTGAGGCAGGAAAATCACTTGAA10.423728813559322No Hit
GATGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCATT10.423728813559322No Hit
GCCCTGTAATCCCAGCTCCTCAGGAGGTTGAGGCAGGAGAATCGTTTGAA10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTCCGGACACGGAGCAGGCCAAACTGATCGGA10.423728813559322No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAACCCCTGCAGTCATA10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTCAGGAGGCTGAGGAAGGAGAATCTCTTGAA10.423728813559322No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGAGCGGGGTAACTC10.423728813559322No Hit
GTTAAAATTTTGGGTTATTTTTTGAATTTTTATTTAGATCGGAAGAGCGG10.423728813559322No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGTTCTGTCGAGACCAT10.423728813559322No Hit
GTTTGTAATTTTAGTTTTTTGGGAAATTGATGTGGGTGGATTATTTGAGG10.423728813559322No Hit
CGATGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCTT10.423728813559322No Hit
CGATGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCTAT10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGTAGTCCACAAGATAGCAGCTGCCCCGACGGGG10.423728813559322No Hit
GCACTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAA10.423728813559322No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGAG10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTTCAGACACGGAGCAGGTCAAACTGATCGGA10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGGGCCAGCTGGTACTACTCTGCT10.423728813559322No Hit
CGGGTCACCTTCTGAATTTTCACTCTTTCCCTACACGACGCTCTTACATC10.423728813559322Illumina Single End PCR Primer 1 (96% over 27bp)
CGATGTATCCAAGTATGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCAT10.423728813559322No Hit
GCCCTGATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACC10.423728813559322No Hit
CGATGTATCCAAGTCTGACGGCCCCAGCAACGGGTGTGCAGGTCGCCCAT10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGTAGTAAAGTCCCCCCATCCGCTCTGACGCACA10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGGAGTCGAATCCCCCTCGAGTGTCTCTTGCACA10.423728813559322No Hit
TGTCGCCTACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGCAT10.423728813559322No Hit
CGGGTCACCTTGCGAATTTTCACTCTTTCCCTACACGACGCTCTTACATC10.423728813559322Illumina Single End PCR Primer 1 (96% over 27bp)
GCCCTGTAATCCCAGTTCCCCGGGAGGCTGAGGTCGGCGGATCGTGGGTG10.423728813559322No Hit
CGATGTATCCAAGTCTGACGGCCCCAGAACCGGGCGTGCAGGGCGCCCAT10.423728813559322No Hit
TGTCGCCTGCACTAAGAGAAACGCAACGAACAGCTTTGTCAATGCATTGC10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGTGGACGTAGTCACCGTAGTCATTTCGTTCTAC10.423728813559322No Hit
GCCCTGTAATCCCAGCACTTTGGAGGCTGAGGTGTGTGGATCACTTGAGG10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTTGGGAGGCTGAGGTGGGAGAATTGCTTGGT10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGTAAGTGTAGACGTTCCCACTACCCCCCCCTCT10.423728813559322No Hit
GCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTACCGCTTCGGCG10.423728813559322No Hit
CGATGTATCCAAGTCTGACGGCCCCAGAAACGGGGGTGCAGGGCGCCCAT10.423728813559322No Hit
CGATGTAGCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCAT10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGACGGTTCCCCTGTCCCAGCCAC10.423728813559322No Hit
GTTTGTAATTTTAGTTTTTTGAGTGATTGAATATGCGGTAGTAATTTATT10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATTAATCCACGTAGTTTCCGATCCCG10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTTGGGAGGCTGGGGCACAAGAATATCTTGAA10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGTAGTCGTAACCATAGCTGTACCTTTCTCTCGC10.423728813559322No Hit
GTGGTCCCTTGGCCCCAGACGTCGATACCCCTCTGGCCAAAATATGCATC10.423728813559322No Hit
CCACACATCCCCGCAGGACTCTCGGGCTCCACCTCCTGCTCGGCCCAGCC10.423728813559322No Hit
CGATGTATCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCATT10.423728813559322No Hit
TGTCGCCCGCACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGC10.423728813559322No Hit
GCCCTGTAATCCCAGCACTTTGGGAGGGTGAGGCAGGTGGATCACACGGA10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTCTGGGAGGCTGAGGCAGGGAGAATCGCTTC10.423728813559322No Hit
CGGGTCACCTTCTGAATTTTTACCTTCATAAACAGCAAGGACTTCGCTCT10.423728813559322No Hit
TGTCGCCGCACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGCA10.423728813559322No Hit
AGGGTTCCCCGCAACCCGGGCCAGCTGCTGCCCCCCCCAGATCTCTCGCA10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCCGGGGGATCCCTTGAG10.423728813559322No Hit
AGGGTTCCCTGGCCCCAGTGCTGGAAGTATTCCTTTTTCGCCACCTCCAA10.423728813559322No Hit
CGATGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTACAGGGCGCCCAT10.423728813559322No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGGGGTTTTAAGGAGCA10.423728813559322No Hit
GCCCTGTAATCCCAGCTACTTGGGGGGCTGAGGCAGGAGAAGCGCTTGAA10.423728813559322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)