FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length208
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCAT1213.636363636363635No Hit
TGTCGCCTGCACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGC1112.5No Hit
GCCCTGTAATCCCAGCTCCCCGGGAGGCTGAGGTCGGCGGATCGTGGGTG910.227272727272728No Hit
CGGGTCACCTTCTGAATTTTTACCTTCATAAACAGCAAGGACTGCGCTCT66.8181818181818175No Hit
AGGGTTCCCTGGCCCCAGTAGTCGGGCGACCCCATGATCCCCCTACTTCT11.1363636363636365No Hit
GTGGTCCCTTGGCCCCAGACGTCCACGGCGGTGTTCTAGCACATCTCAGG11.1363636363636365No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCTATCGTCCC11.1363636363636365No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC11.1363636363636365No Hit
AGGGTTCCCGGGCCCCAGTAGTCATTGGAGTTGGGGGACGAGATAACATA11.1363636363636365No Hit
CGGGTCACCTTCTGCATTTTTACCTTCATAAACAGCAAGGACTGCGCTCT11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCAGCCACTGCTACTCTCCGTTC11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTCCCACA11.1363636363636365No Hit
GTTAAAATTTTGGGTTATTTTTTGAATTTTTATTTAGATCGGAAGAGCGG11.1363636363636365No Hit
GCCCTGTAATCCCAGCTACTCTGGAGGCTGAGACAGGAGAATCGCTTGAA11.1363636363636365No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGTTCCG11.1363636363636365No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGGAGTAGTAATGGTG11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACAGTTAGCGGTTAACCAC11.1363636363636365No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCTCTCCT11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCACCCCTGGTCACCAATGCCACG11.1363636363636365No Hit
AGGGTTCCTTGGCCCCAGGGGTCAAAACCACGCACCATAGAAGATCGCAT11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCAAATATCGTAACCTTTGAT11.1363636363636365No Hit
CGGGTCACCTTCTGAATTTTCACTCTTTCCCTACACGACGCTCTTACATC11.1363636363636365Illumina Single End PCR Primer 1 (96% over 27bp)
AGGGTTCCCTGGCCCCAGTAGTCAAAGACCGCTAAGACCTAGGAACTCCG11.1363636363636365No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCAATCAGGGCCACTCCCCCGGC11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCGCTGAGTTGGGTAGCACTACC11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCCATACCGTACTAGTAGCCGCGCCGCTC11.1363636363636365No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGGAATA11.1363636363636365No Hit
CGATGTATCCAAGTCTGACCCACAGTCAAGTCTGCAGACCGACAGGGGAC11.1363636363636365No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATACGTTACTTTACC11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCATAGTAAAGAGCACTACTATCTAGGCC11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCAGTAAAGTCGATGGTAGATACT11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATGGGAAAATAACCAGTCCCCCTGAA11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAGCACAACAATGCAATACTATAATA11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCACCCAACGAAGTAATAACCACT11.1363636363636365No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGCAGTAGTAGTAGTAGTTTTTGGC11.1363636363636365No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCTGCCCCCGTAGCTCTCACCC11.1363636363636365No Hit
GCCCTGTAATCCCAGCTACTACGGAGGCTGAGACAGAATTGCTTGAACCC11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAGCACTTTTGGACGATCCTCCTATA11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGCAGTATTACCAGCTGCTGCTA11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA11.1363636363636365No Hit
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGTAAGGTGAGATGTTTTG11.1363636363636365No Hit
GCCCTTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGCAGAATCGCTTGA11.1363636363636365No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCATATCACTGAACTCTACCC11.1363636363636365No Hit
GCCCTGTAATCCCAGCTACTCTGGAGGCTGAGGCAGGAGAATTGCTTGAA11.1363636363636365No Hit
GCCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAA11.1363636363636365No Hit
AGGGTTCCCTGGCCCCACTGGTCAAAGTCGTTCGTAGATGTCCCCAGATC11.1363636363636365No Hit
GTGGTCCCTTAGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCCCATA11.1363636363636365No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGCCGGCCTGGCCAGCCGGGACGAT11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCCTGTGACCAGTCACTGAT11.1363636363636365No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGAGTAGTTGGCGATGGAATA11.1363636363636365No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGAAGTAGTAGGTCATCGC11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGAGGGGGACCCTGAGGTAGTAGTACACATCGGT11.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACATCATGGTGGTATTGTCACA11.1363636363636365No Hit
GCCCTGTAATCCCAGCTACTTGGGAGTCTGAGGTGGGAGGATGGCTTGAG11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)