FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853442
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT17925221.003419095849512Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT837089.80828222656021No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT533866.255375292052652Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT512496.004977491147612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT338703.968635244105633No Hit
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT310323.6360994654586953Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT232442.723559421729889Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC168791.9777559576397694No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT145911.7096650973352612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT112101.3135046083975244Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG95371.1174748840577333No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT91161.0681452283810733No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT88271.0342823531065966Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC76560.8970732633266233No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT67720.7934927036635179Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC62730.7350235868401134Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC61320.7185022532286904No Hit
CTTCCCTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC58120.6810070280112767Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG55750.6532371268346296No Hit
CTTTCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT50900.596408426114487Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG47150.5524687090628303No Hit
CTTCCCTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC45180.52938571103836Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT43940.5148563112666121Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC33650.3942857276768661Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT32310.3785846021170742No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT32180.37706135859261675No Hit
CTTACNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT29950.35093187351923155Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGGACCAGATCGGAAGAGCACACGTCTGAACTC27880.326677149706717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC27630.3237478352366066No Hit
CTTCCCTGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC26010.3047658774702909Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT25140.29457186311430655No Hit
TTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT22180.25988877978819885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC18890.22133900136154538Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT18790.2201672755735012No Hit
CTTCCCTGGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC18760.21981575783708796Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG18470.21641775305175978No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG16860.19755296786424853No Hit
TTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT16830.19720145012783527No Hit
CTTACCTGAGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAAC16610.1946236533941381Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT16270.1906397857147879Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCNTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC15600.1827892229348919Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA15580.18255487777728305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT13520.15841732654357296Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGCGGACCAGATCGGAAGAGCACACGTCTGAACTC13210.154784976600636Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCCNTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC12670.14845765734519745Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCAAGATCGGAAGAGCACACGTCTGAACTC12380.14505965255986933Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGGCTGAACT12240.14341923645660748Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTTACNTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT11670.13674039946475566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC11530.13509998336149381Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAACT11520.1349828107826894Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT10940.12818680121203316Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGACCAAGATCGGAAGAGCACACGTCTGAACTCCA10690.12525748674192272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGCGCACACGTCTGAACT10580.12396858837507412Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTTACNTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC10370.12150796422018134Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA10350.12127361906257249No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT9480.11107960470658815No Hit
CTTACCTGAGGAGACGGTCCAAGATCGGAAGAGCACACGTCTGAACTCCA9140.10709573702723793Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)