FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715211
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT7501610.488652998905218No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT617578.634794487221253Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC423025.914618203579083No Hit
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT293204.099489521274142No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC212012.964300045720773No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT199682.791903368376605No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT173352.423760260957955Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC144072.0143705843450395No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG133271.8633661954304395No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT108901.5226275882222169Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT96491.3491123598490515No Hit
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT86971.2160047873984041Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT83791.171542383995772Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC73911.0334013319146378No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG70820.9901972984196271No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT69890.9771941427075368No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT61800.8640806699002113No Hit
CTTACCTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA50610.7076233447192506Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG45890.6416288340084255No Hit
CTTACCTGAGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAAC43390.606674114352268Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT43230.6044370122942739Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG42180.5897560300386879No Hit
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC40400.5648682696435039Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG35440.4955181058456875No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT34470.4819556746190984No Hit
CTTACCTGAGGAGACTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT31900.4460222228125686Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT31660.44266656972557744Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG27220.3805869876162419No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT24810.3468906378677062Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT24110.3371033163639821No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT23480.3282947270106304Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC23380.32689653822438414No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT20630.288446346602611No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA19260.2692911602310367No Hit
CTTACCTGAGGAGACGGTGACCGAGGTCGAGGGGCTGGCGGAAGGGACAG17830.24929706058771467No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC15590.21797763177579763No Hit
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG15480.2164396241109267No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG14620.20441520054920856No Hit
CTTACNTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA13860.19378896577373672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTACCTGGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAACT13250.1852600141776343Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC13100.18316273099826486Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG13090.18302291211964022No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT12300.1719772207082945No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT12020.16806229210680484No Hit
CTTACCTGAGGAGACGGTGACCACGAAGGCGGCAAAGGCGACGGAATGGA11730.16400754462669057No Hit
CTTACNTGAGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAAC11530.161211167054198Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTACNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT11530.161211167054198Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC10600.14820801134210743Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA10010.13995869750325426No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA9930.13884014647425724No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG9910.13856050871700798No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC9520.13310757245064742No Hit
CTTACCTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT9090.12709536066978835Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG8820.12332025094692337No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC8760.12248133767517558No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT8710.12178224328205244No Hit
CTTACCTGAGGAGCCTGTCGCACCCAGAGATCGGAAGAGCACACGTCTGA8520.11912568458818447Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTACNTGAGGAGACTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT8490.11870622795231059Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT8480.11856640907368596No Hit
CTTACNTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG8300.11604966925844262No Hit
CTTACCTGAGGGGCCTGTCGCACCCAAAGATCGGAAGAGCACACGTCTGA7980.11157546514245445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTACCTGAGGAGCCTGTCGCACCCAAAGATCGGAAGAGCACACGTCTGA7980.11157546514245445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTACCTGAGGAGCCTGGCGCACCCAGATCGGAAGAGCACACGTCTGAAC7820.10933836308446038Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC7640.10682162326921706No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG7530.10528361560434613No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG7390.10332615130360132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)