FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485673
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT10485521.58962923613213No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT5354711.02531950509911Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT412368.490486397226118No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC185293.815118402711289No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT158283.2589828958990923Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG101382.087412724199204No Hit
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT94771.9513129204217654Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT89051.83353820368849No Hit
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT85531.7610614549295511Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC84411.7380006712335254No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC63891.3154941699456217No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG58751.209661644769217No Hit
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG51721.0649140471057688No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT37590.7739775527978702No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT34460.7095308983616548No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT31050.639319048001433No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC30160.6209939609572697No Hit
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT27430.5647833006982064No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT26590.547487712926187Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT24380.5019838450974214Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG20270.41735900492718353No Hit
CTTCCCTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC20130.4144764069651803Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT17490.3601188453959763No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT16990.34982385267453614Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG15640.32202737232664774No Hit
CTTCCCTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC14480.2981429892129066Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT13830.2847594986750344Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT13490.2777589036244551Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT13230.2724055074093063No Hit
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC10900.2244308413273952Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT10620.21866564540338868Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC10500.21619484715024306No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA10130.20857655253637736No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT9350.19251636389093074No Hit
CTTCCCTGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC8530.1756325758277689Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC8360.17213227830247924No Hit
CTTACCTGAGGAGACGGTGACCACAGGCGGAGAACACTGACAGCATCCTT7220.1486596948975957No Hit
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC6730.13857060203058436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG6520.13424670508757952No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT5820.11983371527756329No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC5570.11468621891684322No Hit
CTTACGTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT5550.11427441920798562No Hit
CTTCCCTGGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC5540.11406851935355682Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT5510.1134508197902704No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG5380.11077412168269599No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG5320.10953872255612315No Hit
CTTCCNTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC5140.10583252517640471Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT5120.10542072546754709Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCACAGGTGGATAACACTGACAGCATCCTT4930.10150862823339983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)