FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410284
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT15600811.062169038292996No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC973666.9039994781193No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT694274.9229091445411Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT621894.409679185185396No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC546283.8735460375357023No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT453443.215238916416835No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC339432.40682018657235No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG302692.146305283191187No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT261601.8549455287020202No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT197481.400285332599675Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC193081.3690859429731883No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT157111.1140309327766607No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT134720.9552685842000619No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT130440.9249200870179339Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG121950.8647194465795541No Hit
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT113030.8014697748822223Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT105240.7462326737026017Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG104350.7399218880736078No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT96750.686032033264222No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG87610.6212223920855657No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT72370.5131590516520077No Hit
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG60810.4311897461787838No Hit
CTTACCTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA57720.4092792657365467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT57530.407932019366312Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAAC49520.3511349487053671Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC48820.34617140944660796No Hit
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC47710.33830065433628975Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA47060.33369165359601327No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC46890.3324862226331718No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT46510.32979172989270245No Hit
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG46440.3292953759668265No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT39460.27980179878662736Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT37370.26498208871404627No Hit
CTTACCTGAGGAGACTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT35710.25321140990041724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGAGGTCGAGGGGCTGGCGGAAGGGACAG34450.24427703923465058No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT33800.23966803849437418No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG31810.2255574054587587No Hit
CTTACCTGAGGAGACGGTGACCACGAAGGCGGCAAAGGCGACGGAATGGA31400.22265018960719968No Hit
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG31280.22179929716284094No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG31140.22080658931108912No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT31010.21988478916303383Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT30880.21896298901497852Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC25310.17946739805599438No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT24130.17110028901980026No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT23390.16585311894625482No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA22300.15812417924332972No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG22040.15628057894721914No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG20890.1481261930221147No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC20540.1456444233927351No Hit
TTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC19410.13763185287502375No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG18440.13075380561645739No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC17620.12493937391333944No Hit
CTTACCTGGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAACT17610.12486846620964288Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC16920.1199758346545802No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA16470.116784987988235No Hit
CTTACNTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA16300.11557955702539346Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC16140.1144450337662485No Hit
CTTACNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT15260.1082051558409512Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC14990.10629064784114406Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT14810.10501430917460597No Hit
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC14720.10437613984133692Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA14470.10260344724892291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)