FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001132664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786748
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC25124414.061524064949282No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC1356147.5899903064114245No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC998475.58819710446017No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT909885.092380122994402No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG675323.7796040628001264No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC552053.089691439419549No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT499142.7935668600160737No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT374042.0934121655655975No Hit
CTTANCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG362532.0289934562680356No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT203641.1397242364340132No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG176900.9900668700902421No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC157330.8805382740039445No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT142220.7959712281754338No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC116590.6525262655953722No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG97320.5446766975533204No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA92190.5159653179967181No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC86070.4817131458941048No Hit
CTTANCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT75500.4225553911351797No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG74450.4166787929803195No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC71880.40229511940128093No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC66390.37156890619158384No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT55560.3109559937943123No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT55070.3082135813220443No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT50100.28039768338903975No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA50040.2800618777801906No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC48840.2733457656032076No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG48410.2709391587397887No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA42330.2369108570430749No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG41870.2343363473752314No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG40100.2244300819141815No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA38410.21497155726493047No Hit
CTTANCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC35980.20137143010653993No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG33650.18833097896289794No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT33120.18536469608473047No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA32560.18223051040213842No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT31770.17780906988562462No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC30340.16980570287471988No Hit
CTTANCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT30150.16874231844669757No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT29550.16538426235820608No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC29090.1628097526903626No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT28450.1592278261959717No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC27770.1554220292956813No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG27160.15200800560571495No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC26940.1507767183732681No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG26400.14775446789362573No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA23780.13309095630721288No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG21700.12144969520044237No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG21670.12128179239601779No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA19640.10992036929662156No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG19360.10835327645532554No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG19220.10756973003467753No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT18650.10437957675061062No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC18240.10208490509014143No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT18150.1015811966768677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)