FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001132668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645793
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC24074614.627963540979941No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC1266747.69683672247968No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC853565.1863144393006895No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT789004.794041534992554No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG607793.692991767494454No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC455622.7683918937557763No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT423742.5746858809096893No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT367862.235153509584741No Hit
CTTANCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG317011.9261839125576545No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT196071.1913405877895944No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG156880.9532182965901544No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT132530.80526530371681No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC107510.6532413250025976No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC85760.5210861876311298No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA84870.515678460170872No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG84450.513126498897492No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC72320.43942342688296765No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG71220.4327397187860199No Hit
CTTANCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT69350.42137741502120857No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC60670.3686368820380206No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT54010.32817006756013667No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT50810.30872655309628855No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC49350.2998554496221578No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC45390.2757941004731458No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA43880.26661919208551743No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT41770.2537986247359176No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG41290.25088209756634033No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA41090.24966687791234984No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG40680.2471756776216693No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG37580.22833977298481645No Hit
CTTANCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC35570.21612681546221185No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC35040.21290648337913698No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT34860.21181278569054554No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG33990.20652658019568682No Hit
CTTANCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT28070.1705560784375678No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC27640.16794335618148817No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT26940.16369008739252142No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC25680.1560342035723812No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT25160.1528746324720059No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC24960.15165941281801537No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT23980.1457048365134619No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT23840.14485418275566855No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG23770.14442885587677187No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA23550.1430921142573823No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG22830.1387173235030165No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT22500.13671221107393214No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG22330.1356792743680402No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA21430.13021078592508292No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC21060.1279626295652005No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG20230.12291946800113988No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC19830.12048902869315886No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC18630.11319771076921581No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA18160.11034194458233812No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC17990.1093090078764462No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT17660.10730389544736185No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG17440.1059671538279723No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGG17070.10371899746808985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)